Deciphering the roles of subcellular distribution and interactions involving the MEF2 binding region, the ankyrin repeat binding motif and the catalytic site of HDAC4 in Drosophila neuronal morphogenesis

Table 2 Frequency of mushroom body defects resulting from expression of HDAC4^WT or mutants in the mushroom body at 27 °C

Genotype	n	Total β lobe fusion	Severe β lobe fusion (%)	Moderate β lobe fusion (%)	Minor β lobe fusion (%)	Thin or absent lobe(s) (%)	No defects (%)
HDAC4::EGFP/Y ;GAL80ts/ + ;OK107/ +	30	0	0	0	0	0	100
HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-HDAC4^WT/ + ;OK107/ +	19	95	84	0	11	5	0
HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-HDAC4^3SA/ + ;OK107/ +	23	87	74	9	4	13	0
HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-HDAC4^ΔMEF2/ + ;OK107/ +	23	30	4	0	26	4	65
HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-HDAC4^ΔNLS/ + ;OK107/ +	24	71	17	29	25	0	29
HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-HDAC4^ΔANK/ + ;OK107/ +	20	100	65	25	10	0	0
HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-HDAC4^Y1142H/ + ;OK107/ +	24	96	79	8	8	0	4

The percentage of brains displaying each of the defects is shown. The percentage of brains displaying a single phenotype of severe, moderate or minor β lobe fusion, or thin/absent lobe(s) is shown. No brains displayed both β lobe fusion and thin or absent lobes. The total percentage of brains displaying β lobe fusion is also calculated by combining minor, moderate and severe β lobe fusion, and those brains with both β lobe fusion and thin or absent lobes

ISSN: 1741-7007