Deciphering the roles of subcellular distribution and interactions involving the MEF2 binding region, the ankyrin repeat binding motif and the catalytic site of HDAC4 in Drosophila neuronal morphogenesis

Table 3 Frequency of mushroom body defects resulting from expression of HDAC4^WT and mutants in the mushroom body in an HDAC4-depleted background at 27 °C

Genotype	n	Total β lobe fusion (%)	Severe β lobe fusion (%)	Moderate β lobe fusion (%)	Minor β lobe fusion (%)	Thin or absent lobe(s) (%)	No defects (%)
Control HDAC4::EGFP/Y ;GAL80ts/ + ;OK107/ +	30	0	0	0	0	0	100
HDAC4 KD HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-deGradFP/ + ;OK107/ +	25	20	4	8	8	0	80
HDAC4 KD + HDAC4^WT HDAC4::EGFP/Y ;GAL80ts/ + ;UAS deGradFP/UAS-HDAC4^WT;OK107/ +	30	33	7	13	13	0	67
HDAC4 KD + HDAC4^3SA HDAC4::EGFP/Y ;GAL80ts/ + ;UAS-deGradFP/UAS-HDAC4^3SA;OK107/ +	19	74	26	42	5	16	11
HDAC4 KD + HDAC4^ΔMEF2 HDAC4::EGFP/Y ;GAL80ts/ + ;UAS deGradFP/UAS-HDAC4^ΔMEF2;OK107/ +	20	10	5	0	5	0	90
HDAC4 KD + HDAC4^ΔNLS HDAC4::EGFP/Y ;GAL80ts/ + ;UAS deGradFP/UAS-HDAC4^ΔNLS;OK107/ +	41	37	15	0	22	0	63
HDAC4 KD + HDAC4^ΔANK HDAC4::EGFP/Y ;GAL80ts/ + ;UAS deGradFP/UAS-HDAC4^ΔANK;OK107/ +	21	62	19	19	24	0	38
HDAC4 KD + HDAC4^Y1142H HDAC4::EGFP/Y ;GAL80ts/ + ;UAS deGradFP/UAS-HDAC4^Y1142H;OK107/ +	23	51	4	4	43	0	48

The percentage of brains displaying a single phenotype of severe, moderate or minor β lobe fusion, or thin/absent lobe(s) is shown. Flies were raised at 27 °C. No brains displayed both β lobe fusion and thin or absent lobes. The total percentage of brains displaying β lobe fusion is also calculated by combining minor, moderate and severe β lobe fusion, and those brains with both β lobe fusion and thin or absent lobes. KD, knockdown; OK107 = OK107-GAL4

ISSN: 1741-7007