Deciphering the roles of subcellular distribution and interactions involving the MEF2 binding region, the ankyrin repeat binding motif and the catalytic site of HDAC4 in Drosophila neuronal morphogenesis

Table 5 Impact of mutation of the MEF2 binding region on the frequency of mushroom body defects resulting from pan-neuronal expression of HDAC4^WT and mutants

Genotype	n	Total β lobe fusion (%)	β lobe fusion and thin or absent lobe(s) (%)	Severe β lobe fusion (%)	Moderate β lobe fusion (%)	Minor β lobe fusion (%)	Thin or absent lobe(s) (%)	No defects (%)
elav/ +	23	0	0	0	0	0	0	100
elav/ + ;UAS-HDAC4^WT/ +	18	100	6	92	0	0	0	0
elav/ + ;UAS-HDAC4^3SA/ +	26	92	54	35	4	0	4	4
elav/ + ;UAS-HDAC4^ΔMEF2/ +	24	21	0	0	0	21	0	79
elav/ + ;UAS-HDAC4^ΔNLS/ +	25	80	0	4	36	40	12	8
elav/ + ;UAS-HDAC4^ΔANK/ +	24	96	13	79	4	0	0	4
elav/ + ;UAS-HDAC4^3SAΔMEF2/ +	23	12	0	0	4	9	17	70
elav/ + ;UAS-HDAC4^ΔNLSΔMEF2/ +	22	23	0	0	5	18	0	77
elav/ + ;UAS-HDAC4^ΔANKΔMEF/ +	25	36	0	0	0	36	8	56
elav/ + ;UAS-MEF2^WT/ +	18	0	0	0	0	0	90	10

The percentage of brains displaying a single phenotype of severe, moderate or minor β lobe fusion, or thin/absent lobe(s) is shown. Flies were raised at 25 °C. As a measure of severity of the phenotype, the percentage of brains displaying both β lobe fusion and thin or absent lobes is also shown. The total percentage of brains diplaying β lobe fusion is also calculated by combining minor, moderate and severe β lobe fusion, and those brains with both β lobe fusion and thin or absent lobes. elav = elav-GAL4

ISSN: 1741-7007