Deciphering the roles of subcellular distribution and interactions involving the MEF2 binding region, the ankyrin repeat binding motif and the catalytic site of HDAC4 in Drosophila neuronal morphogenesis

Table 6 Increased nuclear accumulation of HDAC4 via co-expression of MEF2 results in increased β lobe fusion compared to HDAC4^WT

Genotype	n	Total β lobe fusion (%)	Severe β lobe fusion (%)	Moderate β lobe fusion (%)	No defects (%)
GAL80ts/ + ;OK107/ +	30	0	0	0	100
GAL80ts/UAS-MEF2^WT/ + ;OK107/ +	29	0	0	0	100
GAL80ts/+ ;UAS-HDAC4^WT;OK107/ +	26	35	15	19	65
GAL80ts/UAS-MEF2^WT/ + ;UAS-HDAC4^WT/ + ;OK107/ +	20	75	40	35	25
GAL80ts/+ ;UAS-HDAC4^ΔMEF2/ + ;OK107/ +	28	7	7	0	93
GAL80ts/UAS-MEF2^WT/ + ;UAS-HDAC4^ΔMEF2/ + ;OK107/ +	30	23	3	20	77

The percentage of brains displaying moderate or severe β lobe fusion is shown. Transgenes were expressed with OK107-GAL4 in the presence of tubP-GAL80ts. Flies were raised at 25 °C to achieve approximately half the maximal induction of expression. The total percentage of brains diplaying β lobe fusion is also calculated by combining all columns containing β lobe fusion

ISSN: 1741-7007