Deciphering the roles of subcellular distribution and interactions involving the MEF2 binding region, the ankyrin repeat binding motif and the catalytic site of HDAC4 in Drosophila neuronal morphogenesis

Table 7 Expression of HDAC4^ΔNES results in increased β lobe fusion compared to HDAC4^WT

Genotype	n	Total β lobe fusion (%)	Severe β lobe fusion (%)	Moderate β lobe fusion (%)	No defects (%)
GAL80ts/ + ;OK107/ +	20	10	5	5	90
GAL80ts/+ ;UAS-HDAC4^WT/+ ;OK107/ +	29	17	3	14	83
GAL80ts/+ ;UAS-HDAC4^3SA/+ ;OK107/ +	24	42	4	38	58
GAL80ts/+ ;UAS-HDAC4^ΔNES/+ ;OK107/ +	27	44	11	33	56

The percentage of brains displaying moderate or severe β lobe fusion is shown. Transgenes were expressed with OK107-GAL4 in the presence of tubP-GAL80ts. Flies were raised at 25 °C to achieve approximately half the maximal induction of expression. The total percentage of brains diplaying β lobe fusion is also calculated by combining all columns containing β lobe fusion

ISSN: 1741-7007