Fig. 2

G4 stabilization by PDS and CX-5461 downregulates expression of TMPRSS2 isoform 1. a Expression levels of TMPRSS2 isoform 1 (left graph) and isoform 2 (right graph) in Caco-2 cell line treated 24, 48, and 72 h with different concentrations of PDS (0–50 μM). b Expression levels of TMPRSS2 isoform 1 and isoform 2 in MCF-7 cell line treated 24 h with different concentrations of PDS (0–20 μM). c Expression levels of TMPRSS2 isoform 1 (left graph) and isoform 2 (right graph) in Caco-2 cell line treated 24, 48, and 72 h with different concentrations of CX-5461 (0–2 μM). d Expression levels of isoform 1 (upper graph) and isoform 2 (bottom graph) in MCF-7 cell line treated 24, 48, and 72 h with different concentrations of CX-5461 (0–2 μM). mRNA levels in the graphs from a to d were normalized to the level of U6 snRNA and GAPDH. WT mRNA levels were scaled to 100%. Error bars represent SEM of at least n = 3 biological independent experiments. e Western blot analysis of protein extracts from Caco-2 (left panel) and MCF-7 (right panel) cells untreated or treated 24 h with 1 μM CX-5461 or 10 μM PDS. Below quantification of n = 3 biological independent experiments ± SEM. Membrane was stained with anti-Tmprss2 and anti-Histone 3 antibodies. The original gels are reported in Additional file 9