Fig. 4

SNPs in the exon 1 (5´UTR) of TMPRSS2 isoform 1 influence protein expression in Caco-2. a Yellow fewer virus (YFV) replication in Caco-2 cells transfected with siRNAs against TMPRSS2 isoform 1 and 2 and only isoform 2. Luciferase plate was read 24 h after electroporation with the YFV replicon [56]. b Schematic illustration of the G4 at exon 1 of TMPRSS2 gene and the four SNPs that partially disrupt the G4 motifs obtained by NCBI. c ThT staining of the oligonucleotide harboring the G4 in the exon1 of the isoform 1 as well as oligonucleotide contained four SNPs that partially disrupt the G4 motifs obtained by NCBI. d Caco-2 cells transfected with psiCHECK™-2 containing the G4 at exon 1 of TMPRSS2 gene and the four SNPs that partially disrupt the G4 motifs obtained by NCBI, cloned upstream of the reporter gene. Expression of psiCHECK™-2 was normalized to the G4_ Iso1 sample