Fig. 1
Evaluation of sgRNAs activity at two target loci in Arabidopsis. A, B Design of sgRNAs targeting the EMB2410 (A) and ROS1 (B) loci, respectively. Lowercase and boldface letters in the sgRNA sequence indicate PAM sequences. The coding sequence is shown in uppercase, the 3′ UTR in lowercase, and the stop codon in square to the target site sequence. EMB-sgRNA5 and ROS1-sgRNA2 have been used in previous studies. C Schematic diagram of CRISPR/Cas9 system construct. The construct consists of one AtU6 promoter-driven sgRNA expression cassette and a DD45 promoter-driven SpCas9 expression cassette. T1 transformants were selected for hygromycin resistance of 35S::HPT. D, E Evaluation of sgRNA activity in T1 transformants targeting the EMB2410 (D) and ROS1 (E) loci, respectively. The numbers in parenthesis represent the number of individual samples analyzed. One way ANOVA and Tukey test were used for difference analysis between data (P < 0.05)