Fig. 2

Multiple-sgRNA strategy for GFP knock-in. A Diagram of GFP-KI donor construct and primers for screening. The single sgRNA donor construct consists of one AtU6 promoter-driven sgRNA expression cassette and a KI GFP sequence with homologous arms of the endogenous target gene. Specific primers are specific for the GT allele, while external and full-length primers can amplify both endogenous and KI GT alleles. B Diagram of GFP-KI donor construct with multiple sgRNAs