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Fig. 3 | BMC Biology

Fig. 3

From: Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis

Fig. 3

Genotyping of GFP-KI GT events with sgRNA combinations. A, B PCR genotyping of precise GFP-KI for the EMB2410 (A) and ROS1 (B) loci in T1 transformants. C, D PCR genotyping of precise GT of the EMB2410-GFP-sg5555 and EMB2410-GFP-sg58910 (C) and ROS1-GFP-sg213 (D) in T2 generation. M; marker, PL; parental line. Single means the single sgRNA donor construct; Double, Triple, Quadruple mean GFP-KI donor construct with multiple sgRNAs respectively

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BMC Biology

ISSN: 1741-7007

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