Fig. 4

Correlation analysis of mutation frequency and GT efficiency. A, B Correlation analysis of mutation frequency and GFP-KI GT efficiency by single sgRNA at the EMB2410 (A) and ROS1 (B) loci. Squares indicate precise GT, triangles indicate total GT events. C, D Correlation analysis of precise and total GT efficiency by single sgRNA for the EMB2410 (C) and ROS1 (D) loci. The four filled circles represent four different single sgRNA donor constructs. E, F Correlation analysis for all precise and total GT events at the EMB2410 (E) and ROS1 (F) loci. A total of fourteen GFP-KI donor constructs with single or multiple sgRNAs were analyzed for each of the two loci. G, H Correlation analysis of mutation pattern and GFP-KI GT efficiency at the EMB2410 (G) and ROS1 (H) loci. Squares indicate precise GT; triangles indicate total GT events