Fig. 6

Off-target effects of multiple sgRNAs in Arabidopsis. A, B Mutation frequency of possible off-target sites in EMB-GFP (A) and ROS1-GFP (B) KI transgenic plants. Mutation frequencies of possible off-target sites (Additional file 3: Table S4, S5) were analyzed by PCR followed by sequencing. ns, not significant. One way ANOVA and Tukey test were used for difference analysis between data (P < 0.05)