Table 1 Knock-in GT efficiencies at the EMB2410 and ROS1 loci
| Â | Construct | Number of transformants analyzed | Total GT | Total GT efficiency (%) | Precise GT (biallelic) | Precise GT efficiency (%) | |
|---|---|---|---|---|---|---|---|
EMB-GFP | Single sgRNA | EMB-GFP-sg5 | 192 | 9 | 4.7 | 1 | 0.5 |
EMB-GFP-sg8 | 357 | 15 | 4.2 | 1 | 0.3 | ||
EMB-GFP-sg9 | 198 | 9 | 4.5 | 3 | 1.5 | ||
EMB-GFP-sg10 | 139 | 0 | 0 | 0 | 0 | ||
Double sgRNAs | EMB-GFP-sg55 | 380 | 18 | 4.7 | 1 | 0.3 | |
EMB-GFP-sg58 | 197 | 61 | 31.0 | 0 | 0 | ||
EMB-GFP-sg59 | 217 | 51 | 23.5 | 4 | 1.8 | ||
EMB-GFP-sg510 | 171 | 20 | 11.7 | 3 | 1.8 | ||
Triple sgRNAs | EMB-GFP-sg555 | 226 | 40 | 17.7 | 5 | 2.2 | |
EMB-GFP-sg589 | 194 | 62 | 32.0 | 2 | 1.0 | ||
EMB-GFP-sg5810 | 233 | 20 | 8.6 | 1 | 0.4 | ||
EMB-GFP-sg5910 | 228 | 13 | 5.7 | 1 | 0.4 | ||
Quadruple sgRNAs | EMB-GFP-sg5555 | 202 | 34 | 16.8 | 3 (1) | 1.5 | |
EMB-GFP-sg58910 | 167 | 52 | 31.1 | 4 | 2.4 | ||
ROS1-GFP | Single sgRNA | ROS1-GFP-sg1 | 300 | 0 | 0 | 0 | 0 |
ROS1-GFP-sg2 | 222 | 35 | 15.8 | 7 | 3.2 | ||
ROS1-GFP-sg3 | 188 | 21 | 11.2 | 3 | 1.6 | ||
ROS1-GFP-sg4 | 218 | 9 | 4.1 | 1 | 0.5 | ||
Double sgRNAs | ROS1-GFP-sg22 | 408 | 30 | 7.4 | 5 | 1.2 | |
ROS1-GFP-sg21 | 204 | 41 | 20.1 | 4 | 2.0 | ||
ROS1-GFP-sg23 | 461 | 57 | 12.3 | 5 | 1.1 | ||
ROS1-GFP-sg24 | 408 | 51 | 12.5 | 4 | 1.0 | ||
Triple sgRNAs | ROS1-GFP-sg222 | 286 | 50 | 17.5 | 2 | 0.7 | |
ROS1-GFP-sg213 | 394 | 55 | 14.0 | 1 | 0.3 | ||
ROS1-GFP-sg214 | 226 | 21 | 9.7 | 2 | 0.9 | ||
ROS1-GFP-sg234 | 276 | 31 | 11.2 | 1 | 0.4 | ||
Quadruple sgRNAs | ROS1-GFP-sg2222 | 263 | 37 | 14.1 | 1 | 0.4 | |
ROS1-GFP-sg2341 | 429 | 40 | 9.3 | 0 | 0 | ||