Fig. 2

Analysis of miRNA response to B. bassiana infection in Ae. Aegypti. A Hierarchical clustering analysis of miRNAs with different expression levels in mosquitoes infected with B. bassiana. PBS, mosquitoes treated with PBS; Bb, mosquitoes infected with B. bassiana. B miRNA/mRNA regulatory networks according to an intersection of the three software programs in Ae. aegypti. Ellipses indicate miRNAs, and V shapes indicate target mRNAs. C Venn diagram depicting the overlap of a total of 101 miRNAs targeting ModSP predicted by three pieces of software tools: PITA, miRanda, and RNAhybrid. D qRT-PCR analysis of transcript levels of miRNA-novel-53 after B. bassiana infection. The qRT-PCR results were normalized to the internal control U6. The results were obtained in triplicate and are presented as mean ± SEM. The Student’s t-test was utilized to calculate the significance of treatment mean differences. *P < 0.05; **P < 0.01. PBS, mosquitoes treated with PBS; Bb, mosquitoes infected with B. bassiana