Fig. 4
Effect of miRNA-novel-53 administration on mRNA expression of ModSP in Ae. Aegypti. The relative miRNA abundance of miR-novel-53 in Ae. aegypti after injection with agomir-53 (A) or antagomir-53 (C). The relative mRNA abundance of ModSP in Ae. aegypti after injection with agomir-53 (B) or antagomir-53 (D). Intrathoracic inoculation of a serial concentrations of either miRNA-novel-53 agomir (E) or antagomir (F) changed ModSP expression in Ae. aegypti. Mosquitoes were injected with 5-pM, 50-pM, and 500-pM agomir or antagomir. The results were measured in triplicate and are shown as mean ± SEM. The significance of treatment differences was determined with the Student’s t-test. *P < 0.05, **P < 0.01