Fig. 5

miRNA-novel-53 modulates the expression of ModSP that participates in antifungal immunity of Ae. Aegypti. The survival rate of Ae. aegypti images injected with agomir-53 (A) or antagomir-53 (C) after infection with B. bassiana conidia. Three repetitions of each experiment were conducted. The Kaplan-Meier approach was used to draw the survival curves, and the P-value was determined by the log-rank (Mantel-Cox) test. *P < 0.05. Protein expression level of ModSP in the Ae. aegypti hemolymph after agomir-53 (B) or antagomir-53 (D) treatment upon B. bassiana infection. LP II was used as the loading control. Agomir-C + PBS, Agomir-C + Bb, and Agomir-C-injected mosquitoes treated with PBS or B. bassiana; Agomir-53 + PBS, Agomir-53 + Bb, Agomir-novel-53-injected mosquitoes treated with PBS or B. bassiana; Antagomir-C + PBS, Antagomir-C + Bb, and Antagomir-C-injected mosquitoes treated with PBS or B. bassiana; Antagomir-53 + PBS, Antagomir-53 + Bb, and Antagomir-novel-53-injected mosquitoes treated with PBS or B. bassiana