Fig. 6

Effects of miRNA-novel-53 knockout on the antifungal immunity of Ae. Aegypti. A Survival rate of miRNA-novel-53 knockout Ae. aegypti after infection with B. bassiana conidia. Three repetitions of each experiment were conducted. The Kaplan-Meier approach was used to draw the survival curves, and the P-value was determined by the log-rank (Mantel-Cox) test. **P < 0.01. 53^−/−-PBS, miRNA-novel-53 knockout mosquito treated with PBS; 53^−/−-Bb, miRNA-novel-53 knockout mosquito infected with B. bassiana. B Expression levels of AMPs in the knockout mosquito line treated with PBS or B. bassiana conidia for 48 h. The qRT-PCR results were normalized to the internal control rps7. The results were obtained in triplicate and are presented as mean ± SEM. The Student’s t-test was used to determine the significance of treatment differences (GraphPad Prism 6). **P < 0.01. 53^−/−, miRNA-novel-53 knockout mosquito