Fig. 1

Loss of multiple LAZY and DRO genes confers branch angle insensitivity to changes in light. Time course of Col wild-type, lazy1 mutant, and lazy1 dro1 dro2 dro3 quadruple mutant grown in A constant dark (left) and constant light (right) conditions. White scale bars indicate 10 mm in length. B Quantified tip angles (tangent at 1 cm from the tip with respect to the stem) and C branch angles (angle to 2 cm from the branch point, with respect to the stem) for Col, lazy1, lazy1 dro1 dro3 triple, and quadruple mutants in constant dark (left) and light (right) conditions. Error bars represent ± standard error of the mean at each time point, with n = 8 (Col), 10 (lazy1), 7 (triple), and 8 (quadruple) branches from four individual dark-grown plants, and n = 8 (Col), 8 (lazy1), 8 (triple), and 6 (quadruple) branches from four individual light-grown plants per time point. Angle change from 0 to 72 h for individual branches was averaged and reported with ± standard error of the mean to the right of each graph. Student’s t-test was used to determine the significant difference between 0- and 72-h time points and is reported in parentheses. *p < 0.05, **p < 0.01, ***p < 0.001