Fig. 2

Loss of multiple LAZY and DRO genes confers root angle insensitivity to changes in light. A Schematic of experiment and measurements. Etiolated seedlings were rotated 90° and grown for 24 h in either constant light or dark and root tip angles were measured with respect to the new gravity vector. Root angles were measured and binned into intervals of 30° around a circle. g indicates the gravity vector. B Root tips of Col, dro1, and lazy1 dro1 dro3 show a significant shift in average angle post-rotation when grown in light versus dark, while loss of four LAZY and DRO genes (lazy1 dro1 dro2 dro3) shows no significant difference. Student’s t-test was used to test for significance, using four replicates of 21–24 seedlings (one whole plate with four rows of 6 seedlings) each. C Histogram showing the proportion of total roots for each genotype corresponding to the angular position around a circle as shown in A. Asterisks indicate a significant difference (p < 0.05) of dark-grown seedlings as compared to light for each interval, using Student’s t-test with a Hommel P-value correction for multiple testing. Error bars indicate ± standard error of the mean (SEM) of the four replicates, each containing 21–24 seedlings. D Representative images of light- and dark-grown seedlings. White scale bars indicate 5-mm length