Fig. 5

Effect of light color and photoreceptor-mediated signaling mutants on LAZY and DRO gene expression. A qPCR expression data for Col seedlings grown in 16L:8D cycles and moved to continuous W, R, B, or FR light for 72 h. Error bars represent SD between 4 biological replicates of 10–12 pooled seedlings (one whole plate with 2 rows of 6 seedlings) per time point. B Fold change expression differences between Columbia wild-type and blue light signaling mutants, cry1 cry2 and phot1 phot2, moved from 16L:8D light cycles to either continuous WL or BL. Significant changes from Col in each light color condition are reported. C Fold change expression differences between Columbia wild-type and red light signaling mutants, phyAB or phyABDE, moved from 16L:8D light cycles to either continuous WL or RL. Significant changes from Ler in each light color condition are reported. D Fold change expression differences between Columbia wild-type and light signal integration mutants, pifQ, hy5 hfr1 laf1, and cop1-6, moved from 16L:8D light cycles to either continuous W, R, or B light. Significant changes from Col in each light color condition are reported. Expression values were calculated relative to UBC21 and represent the average between 4 biological replicates of 10–12 pooled seedlings (one whole plate with 2 rows of 6 seedlings) per comparison. Significant changes are shaded blue for downregulation and red for upregulation. *P < 0.05, **P < 0.01, ***P < 0.001, using Student’s t-test, comparing to the control in each light treatment