Fig. 5

Stabilization of CapZ to early endosomes inhibits ZIKV or MHV infection. A, B A549 cells were transiently transfected with FRB-RAB5 and FKBP-CapZβ, then incubated with rapamycin for 12 h, followed by infection with ~ 1 MOI ZIKV for 48 h. The cells were lysed and subjected to ZIKV-E and GAPDH immunoblot analysis (A). Alternatively, cells were immunolabeled with dsRNA antibodies and subjected to confocal imaging and analysis (B). The scale bar is 5 μm. C 17CL cells were transiently transfected with FRB-RAB5 and FKBP-CapZβ, and they were then incubated with rapamycin for 12 h, followed by infection with ~ 1 MOI MHV for 48 h. The cells were lysed and subjected to Nsp9 and GAPDH immunoblot analysis. The blots, images, and graphs represent data from at least three independent experiments. The difference between the two groups was calculated using the ANOVA test. Differences were considered statistically significant when P < 0.05, ***P < 0.001