Fig. 2

loqs2 originated from two independent loqs dsRBD1 duplication events. A Organization of the double-stranded RNA binding domains (dsRBDs) of Loqs and Loqs2. B Phylogenetic relationships among the dsRBDs of the dsRBPs from Fig. 1A. Phylogeny was inferred by maximum likelihood and tree is rooted at the midpoint for visualization purposes. Branches corresponding to the dsRBDs from the orthology groups of Drosha, mle, DIP1, Adar, Dicer2, Dicer1, pasha, Son, Staufen R2D2 and Loqs-D2 and Loqs-D3 are collapsed to facilitate visualization. Loqs2 dsRBDs and Loqs-D1 clades are colored in grey and pink, respectively. Node values correspond to the percentages of 1000 ultra-fast bootstrap iterations. Node values > 60 are shown. Branch lengths represent substitutions per site. A non-collapsed tree is available on the Additional file 1: Fig. S2. C Correlation matrix showing EMBOSS Needle global identity and alignment percentages from the comparison between Ae. aegypti Loqs and Loqs2 dsRBD amino acid sequences. The dendrogram shows the level of similarity given by the distance matrix of identity percentages. D Heatmap showing the structural comparison between Loqs and Loqs2 dsRBD models generated using the DALI server. The dendrogram shows the average linkage clustering given by the similarity matrix. E putative model for loqs2 origin