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Fig. 4 | BMC Biology

Fig. 4

From: Neofunctionalization driven by positive selection led to the retention of the loqs2 gene encoding an Aedes specific dsRNA binding protein

Fig. 4

loqs2 expression and subcellular protein localization differs from loqs and r2d2. A-B Subcellular localization of Loqs2-PA, Loqs-PA, Loqs-PB and R2D2 during overexpression driven by the polyubiquitin promoter. Plasmids were transfected individually (A) or co-transfected (B) in Ae. aegypti Aag2 cells clone AF5. C Heatmap showing the tissue-specific expression of loqs, loqs2 and r2d2 among Ae. aegypti, Ae. albopictus and An. gambiae. The accompanying illustration shows the tissues and developmental stages analyzed. Associated numbers correspond to numbers in the heatmap. Gene expression between tissues was used for hierarchical clustering. White boxes are indicative of no detected mRNA expression

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