Fig. 4

Manhattan distances comparison between generation 1 sister-cells and non related cells. (A) Boxplots of Manhattan distances between the generation 1 CD34+ sister and non related cells. CD34+ sister-cells (43 couples) are in orange and CD34+ non related cells (3612 couples) in green. Manhattan distances were computed using all the 83 selected genes. Statistical comparison was performed using Wilcoxon test. (B) Boxplots of Manhattan distances between generation 1 T2EC sister and non related cells. Manhattan distances were computed between all cells from the same biological conditions using all the 1177 selected genes. Self-renewing sister-cells (30 couples) are in light orange and self-renewing non related cells (1740 couples) in light green, differentiating sister-cells (32 couples) are in orange and differentiating non related cells (1984 couples) in green. Statistical comparison was performed using Student t-test. (C) Histograms of mean Manhattan distances of 1000 random subsampling of distances between 43 CD34+ non related cell pairs (green), compared to the mean distance between the 43 CD34+ generation 1 sister-cells pairs (orange line). (D) Histograms of mean Manhattan distances of 1000 random subsampling of distances between 30 T2EC self-renewing non related cell pairs (light green histogram), compare to the mean distance between the 30 T2EC self-renewing generation 1 sister-cells pairs (light orange line). (E) Histograms of mean Manhattan distances of 1000 random subsampling of distances between 32 T2EC differentiating non related cell pairs (Green histogram), compare to the mean distance between the 32 T2EC differentiating generation 1 sister-cells pairs (orange line)