Fig. 5
Expression and functional analyses of the OsMYB22 gene in response to BPHs. A Expression of OsMYB22 after BPH infestation as revealed by qRT-PCR (n = 3). The expression level at 0 h was set as 1.0, and asterisks indicate significant differences comparing with 0 h as determined by Student’s t test (**, P < 0.01). Both Ubiquitin and Actin genes were used as references in this result. Individual data values are provided in table S14. B Expression of the OsMYB22 gene in MYB22OE lines and WT plants as revealed by qRT-PCR (n = 3). The expression level in ZH11 was set as 1.0, and asterisks indicate significant differences compared with ZH11 plants as determined by Student’s t test (**, P < 0.01). Individual data values are provided in table S15. C Sketch map indicating the sequences of DNA edited in MYB22KO lines, MYB22KO-1, and MYB22KO-2. D Individual tests of MYB22OE-1 and MYB22OE-2 lines in comparison with ZH11. E Small population assays of MYB22OE-1 and MYB22OE-2 lines in comparison with ZH11. F Statistical analysis of the survival rates of plants in the small population assay in E. Asterisks indicate significant differences compared with ZH11 plants as determined by Student’s t test (**, P < 0.01). Individual data values are provided in table S16. G Individual tests of MYB22KO-1 and MYB22KO-2 lines in comparison with ZH11. H Small population assays of MYB22KO-1 and MYB22KO-2 lines in comparison with ZH11. I Statistical analysis of the survival rates of plants in the small population assay in H. Asterisks indicate significant differences compared with ZH11 plants as determined by Student’s t test (**, P < 0.01). Individual data values are provided in table S17. J, K Expression of OsMYB22 (J) and OsmiR319b (K) in hybrid plants as revealed by qRT-PCR and miRNA qRT-PCR (n = 3), respectively. The expression level in ZH11 was set as 1.0, and asterisks indicate significant differences compared with ZH11 plants as determined by Student’s t test (**, P < 0.01). Individual data values are provided in table S18. L Individual tests of the WT, miR319bOE, and cross plants miR319bOE/MYB22OE