Fig. 3

Loss of conventional ER to Golgi transport via ERGIC-53 disruption does not negatively affect Or22a packing into puncta nor its overall transport. A, B ERGIC-53-knockdown almost completely diminishes Orco in the cilia (orange arrowheads), but Or22a is not significantly affected. C, D Magnification of isolated regions (purple squares) demonstrates that the Or22a puncta-mediated transport continues in spite of Orco puncta abrogation (circled yellow). E Orco puncta frequency is significantly reduced in ERGIC-53 knockdown antennas in comparison to controls, but Or22a remains unchanged. F Orco, but not Or22a, fails to be transported into the cilia and is retained in the soma in ERGIC-53-IR neurons. Control n = 8; ERGIC-53-IR n = 8. For all, error bars represent mean SEM. Two-tailed Student’s t-test was applied for statistical analyses