Fig. 6
From: A cilia-bound unconventional secretory pathway for Drosophila odorant receptors
Grasp65, but not GM130 or intact somatic Golgi, is required for Or22a puncta formation. A, B Somatic Or22a puncta are lost in Grasp65[102] and GM130[∆23] mutants, with no changes to dendritic puncta or cilia vs soma Or22a localization. For Wild-type, antennal n = 7; for Grasp65[102], n = 9; for GM130[∆23], n = 6. C Representative images of GM130 distribution in control w1118 or homozygous Grasp65[102] and GM130[∆23] mutants. GM130 is a cis-Golgi localized membrane protein without which the Golgi could not compartmentalize into cis, medial, and trans stacks and is thus often used as a marker to convey Golgi apparatus integrity. Here, although somatic Golgi stacks are completely ablated GM130[∆23] mutants, they are retained in Grasp65[102] mutants, despite the reputed function of Grasp65 as a Golgi stacking protein. D–F The integrity of somatic and dendritic GM130-positive structures is compromised in GM130[∆23] but not Grasp65[102] mutants. In D, Wild-type somatic structures measured were n = 225; for Grasp65[102], n = 377; for GM130[∆23], n = 118. In E and F, Wild-type, antennal n = 7; for Grasp65[102], n = 9; for GM130[∆23], n = 6. Soma puncta are marked by blue arrowheads, and dendritic puncta by yellow arrowheads. Scale bars are as shown. Error bars represent mean SEM. One-way ANOVA with multiple comparison test and Dunnett’s correction was applied for statistical analysis