Fig. 6

GATA3 promotes HR-mediated DNA damage repair and restores the efficiency of HR in Brca1-deficient cells. a, b The HR and NHEJ reporter cell lines, DR-GFP U2OS (a) and EJ5-GFP U2OS (b), were transfected with pBabe-Empty (Empty) or pBabe-GATA3 (GATA3), and then infected with virus expressing I-SceI. Two days later, cells were collected and analyzed for GFP expression by FACS (left panels) or for expression of GATA3 by qRT-PCR (right panels). c, d DR-GFP U2OS (c) or EJ5-GFP U2OS (d) cells were transfected with si-GATA3-1, si-GATA3-2 or si-control (si-Ctrl), infected with I-SceI, and analyzed for GFP (left) and GATA3 (right). The results in a–d represent the mean ± SD of three independent experiments. The asterisk (*) denotes a statistical significance from Empty and GATA3 or si-Ctrl and Si-GATA3 samples determined by the T-test. The number sign (#) denotes a statistical insignificance from Empty and GATA3 or si-Ctrl and Si-GATA3 samples determined by the T-test. e DR-GFP U2OS cells were firstly transfected with si-Ctrl or si-BRCA1 for 8 h and then transfected with pBabe-Empty (Empty) or pBabe-GATA3 (GATA3) for an additional 8 h. HR assay was done as above described (left) and gene expression was determined by qRT-PCR (right). f DR-GFP U2OS cells were firstly transfected with si-Ctrl or si-GATA3 for 8 h and then transfected with pBabe-Empty (Empty) or pBabe-BRCA1 (BRCA1) for an additional 8 h. HR assay was done as above described (left) and gene expression was determined by qRT-PCR (right). The results in e represent the mean ± SD of three independent experiments. The asterisk (*) denotes a statistical significance from Si-Ctrl + Empty and Si-Ctrl + GATA3, Si-BRCA1 + Empty, or Si-BRCA1 + GATA3 samples determined by the T-test. The asterisk (**) denotes a statistical significance from Si-BRCA1 + Empty and Si-BRCA1 + GATA3 samples determined by the T-test. The number sign (^#) denotes a statistical insignificance from Si-Ctrl + Empty and Si-Ctrl + GATA3 samples determined by the T-test. The results in f represent the mean ± SD of three independent experiments. The asterisk (*) denotes a statistical significance from Si-Ctrl and Si-GATA3 + Empty samples determined by the T-test. The asterisk (**) denotes a statistical significance from Si-GATA3 + Empty and Si-GATA3 + BRCA1 samples determined by the T-test. The number sign (^#) denotes a statistical insignificance from Si-Ctrl and Si-GATA3 + BRCA1 samples determined by the T-test