Full-length amino acid alignment of functional photoproteins from hydromedusae and ctenophores. EF-hand domains I, III, and IV are indicated by long lines above the alignment, while the 12-residue calcium binding loops within each EF-hand domain are indicated by short lines above the alignment. Calcium-coordinating residues within EF-hand domains (black triangles) and residues in the coelenterazine binding pocket (black circles) are indicated. Residue 6 within the EF-hand I calcium loop is also indicated (black star). Residue numbering is based on the berovin sequence (Ba_berovin). Columns of residues are shaded by similarity group conservation (defined by GeneDoc and the BLOSUM62 matrix) where black shows ≥90%, dark grey shows ≥70% and light grey shows ≥55% similar residues in a column. Species are abbreviated as follows: Mlei = Mnemiopsis leidyi; Ba = Beroe abyssicola; Bi = Bolinopsis infundibulum; Mc = Mitrocoma cellularia; Ol = Obelia longissima; Og = O. geniculata; Cg = Clytia gregarium; Ac = Aequorea coerulescens; Am = A. macrodactyla; Ap = A. parva; Av = A. victoria.