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Answer to Wang and Luo, "Polyploidization increases meiotic recombination frequency in Arabidopsis: a close look at statistical modelling and data analysis"
BMC Biology volume 10, Article number: 31 (2012)
Meiosis is an obligate process during sexual reproduction that involves the combination of parental genomes and the coordinated segregation of the recombined chromosomes to the gametes. Polyploidy, the presence of more than two sets of chromosomes per nucleus, has direct and fundamental consequences on meiosis, which are gradually and individually different between the extreme cases of auto- and allopolyploids (multiplied chromosome sets or combination of slightly different chromosome sets). Polyploidy has a major impact on the segregation of genotypes and phenotypes in progeny. In a recent study , we described that polyploidy increased the frequency of meiotic recombination between two genetically linked transgenes providing seed-specific fluorescence. This increase was seen in reciprocal crosses of genetically identical diploid and autotetraploid Arabidopsis thaliana, but also in reconstituted hybrids resembling allotetraploid A. suecica. In a comment, Wang and Luo  question the validity of the data analysis after subjecting the experimental data to a different calculation method.
It is correct that genetic segregation analysis in autotetraploids requires considering the possibility of multivalent formation during pachytene and a potential double reduction of genetic loci. Both special conditions of autotetraploid meiosis are mentioned in , p. 1-2. We agree that a more detailed tetrasomic linkage analysis could improve the data analysis for segregation in autotetraploids. We lack the mathematical expertise to critically review the different theoretical models presented in  and refer to a detailed comment in . However, the evaluation of meiotic recombination suggested by  also results in significantly higher rates in auto- and allopolyploids on one side and diploids on the other. Therefore, the claim in  for 'qualitative differences from the original analysis' is not justified.
Further, the statement that we 'concluded that meiotic recombination was more frequent in the allotetraploids than in the autotetraploids' is incorrect: we wrote that the increase of recombination frequency of both polyploids over diploids was 'in the same range' (p. 4), which holds true for both different evaluations [1, 2].
We have corrected three values in Table 1 according to the comments in .
In summary, we are confident that the conclusions from our work remain unchanged.
Pecinka A, Fang W, Rehmsmeier M, Levy AA, Mittelsten Scheid O: Polyploidization increases meiotic recombination frequency in Arabidopsis. BMC Biology. 2011, 9:
Wang L, Luo Z: Polyploidization increases meiotic recombination frequency in Arabidopsis: a close look at statistical modelling and data analysis. BMC Biology. 2012, this issue
Rehmsmeier M: Response to Wang and Luo. BMC Biology. 2012, this issue
Melamed-Bessudo C, Yehuda E, Stuitje AR, Levy AA: A new seed-based assay for meiotic recombination in Arabidopsis thaliana. Plant Journal. 2005, 43: 458-466. 10.1111/j.1365-313X.2005.02466.x.
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Pecinka, A., Levy, A. & Mittelsten Scheid, O. Answer to Wang and Luo, "Polyploidization increases meiotic recombination frequency in Arabidopsis: a close look at statistical modelling and data analysis". BMC Biol 10, 31 (2012). https://doi.org/10.1186/1741-7007-10-31
- Sexual Reproduction
- Segregation Analysis
- Recombination Frequency
- Reciprocal Cross
- Meiotic Recombination