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Table 2 Yeast genes comprising the Haploproficient Genome-Integrity (HPGI) set, along with their unique human orthologs, and functional annotations
| Yeast gene | Unique human ortholog | Human ortholog function & OMIM cancer association (where applicable) | Yeast gene product cell cycle-related function |
|---|---|---|---|
| BUB2 | - | - | Mitotic exit network regulator; blocks cell cycle progression before anaphase in response to spindle and kinetochore damage |
| CCR4 | CNOT6 | CCR4-NOT transcription complex, subunit 6 | Component of the CCR4-NOT transcriptional complex |
| CDC34 | UBE2R2 | Ubiquitin-conjugating enzyme E2R 2 | Ubiquitin-conjugating enzyme (E2) and catalytic subunit of SCF ubiquitin-protein ligase complex that regulates cell cycle progression by targeting key substrates for degradation |
| CHK1 | CHEK1 | Serine/threonine kinase and DNA damage checkpoint effector | Serine/threonine kinase and DNA damage checkpoint effector |
| CLB1 | CCNB1 | Cyclin B1 | B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase |
| CTF8 | CHTF8 | Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion | Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion |
| DBF2 | - | - | Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis. |
| DNL4 | LIG4 | DNA ligase required for non-homologous end-joining; OMIM: Lig4 syndrome | DNA ligase required for nonhomologous end-joining (NHEJ) |
| IDP1 | IDH1 | Isocitrate dehydrogenase 1 (NADP+); OMIM: Oligodendroglioma; piloytic astrocytoma | Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate |
| IRR1 | STAG1 | Stromal antigen 1 | Subunit of the cohesin complex, required for sister chromatid cohesion during mitosis and meiosis |
| MAD1 | MAD1L1 | Component of the spindle-assembly checkpoint | Coiled-coil protein involved in the spindle-assembly checkpoint; forms a complex with Mad2p |
| MEU1 | MTAP | Methylthioadenosine phosphorylase; OMIM: Glioma | Methylthioadenosine phosphorylase |
| MLH1 | MLH1 | mutL homolog 1, colon cancer, nonpolyposis type 2 | Protein required for mismatch repair in mitosis |
| MSH2 | MSH2 | mutS homolog 2, colon cancer, nonpolyposis type 1; OMIM: colorectal cancer | Protein required for mismatch repair in mitosis |
| MUS81 | MUS81 | MUS81 endonuclease homolog; OMIM: Bloom syndrome (predisposition to leukemias, lymphomas & carcinomas) | Subunit of the Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability |
| RAD1 | ERCC4 | Excision repair cross-complementing rodent repair deficiency, complementation group 4; OMIM: Xeroderma pigmentosum (predisposition to basal cell carcinoma & melanoma) | Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair |
| RAD54 | RAD54L | RAD54-like; OMIM: Lymphoma, non-Hodgkin | DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; recombinational repair of double-strand breaks in DNA |
| RAD61 | - | - | Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion |
| RAD9 | RAD9 | - | DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p |
| SMC2 | SMC2 | Structural maintenance of chromosomes 2 | Subunit of the condensin complex |
| STP22 | TSG101 | Tumor susceptibility gene 101; OMIM: Breast cancer | Component of the ESCRT-I complex |
| TOP1 | TOP1 | Topoisomerase (DNA) I | Topoisomerase I |
| TOP2 | TOP2B | Topoisomerase (DNA) II beta 180kDa | Topoisomerase II |
| TPD3 | PPP2R1B | Protein phosphatase 2, regulatory subunit A, beta; OMIM: Lung cancer | Regulatory subunit A of the heterotrimeric protein phosphatase 2A (PP2A) |
| TUM1 | TST | Thiosulfate sulfurtransferase (rhodanese); OMIM: Tumours | Rhodanese domain sulfur transferase |
| UBX4 | ASPSCR1 | Alveolar soft part sarcoma chromosome region, candidate 1; OMIM: Alveolar soft-part sarcoma | UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p |
| YCG1 | NCAPG | non-SMC condensin I complex, subunit G | Subunit of the condensin complex |
| RIM11 | GSK3A | Glycogen synthase kinase 3 alpha | Protein kinase required for signal transduction during entry into meiosis |
| NBP2 | SH3GL1 | SH3-domain GRB2-like 1; OMIM: Acute myeloid leukemia | Protein involved in the HOG (high osmolarity glycerol) pathway, contains an SH3 domain that binds Pbs2p |
| PBS2 | MAP2K1 | MAP kinase kinase | MAP kinase kinase of the HOG signaling pathway; mediates cell cycle arrest |
