Copy-number variation of cancer-gene orthologs is sufficient to induce cancer-like symptoms in Saccharomyces cerevisiae
BMC Biology volume 11, Article number: 24 (2013)
Copy-number variation (CNV), rather than complete loss of gene function, is increasingly implicated in human disease. Moreover, gene dosage is recognised as important in tumourigenesis, and there is an increasing realisation that CNVs may not be just symptomatic of the cancerous state but may, in fact, be causative. However, the identification of CNV-related phenotypes for mammalian genes is a slow process, due to the technical difficulty of constructing deletion mutants. Using the genome-wide deletion library for the model eukaryote, Saccharomyces cerevisiae, we have identified genes (termed haploproficient, HP) which, when one copy is deleted from a diploid cell, result in an increased rate of proliferation. Since haploproficiency under nutrient-sufficient conditions is a novel phenotype, we sought here to characterise a subset of the yeast haploproficient genes which seem particularly relevant to human cancers.
We show that, for a subset of HP genes, heterozygous deletion is sufficient to cause aberrant cell cycling and altered rates of apoptosis, phenotypes associated with cancer in mammalian cells. A majority of these yeast genes are the orthologs of mammalian cancer genes, and hence our studies suggest that CNV of these oncogenic orthologs may be sufficient to lead to tumourigenesis in human cells. Moreover, where not already implicated, this cluster of cancer-like phenotypes in this model eukaryote may be predictive of the involvement in cancer of the mammalian orthologs of these yeast HP genes. Using the yeast set as a model, we show that the response to a range of anti-cancer drugs is strongly dependent on gene dosage, such that intermediate concentrations of the drugs can actually increase a mutant’s growth rate.
The exploitation of data on the phenotypic impact of heterozygosis in Saccharomyces cerevisiae has permitted the prediction of CNVs affecting tumourigenesis in humans. Our yeast data also suggest that the identification of CNVs in tumour cells may assist both the selection of anti-cancer drugs and the dosages at which they should be administered if they are to be a beneficial, rather than a deleterious, therapy.
There is an increasing realisation that copy-number variation, and in particular the loss of one copy of a gene from a diploid cell or organism, can have a significant phenotypic impact. Moreover, the importance of gene dosage in tumourigenesis is becoming increasingly recognised , and the widespread aneuploidy and copy-number variations (CNVs) that are the hallmarks of cancer cells are coming to be seen as potentially causative, rather than simply symptomatic [2–4]. Identifying causal CNV-related phenotypes for mammalian genes, however, is hampered by the difficulty in constructing deletion mutants in the higher Eukaryotes, and the fact that homozygous knockout (or complete RNAi knockdown) phenotypes do not necessarily correlate with those elicited by the loss of a single copy of a gene [5, 6].
In the model eukaryote Saccharomyces cerevisiae, in contrast, high-throughput screens on whole-genome libraries have facilitated the identification of yeast genes for which CNV has a significant effect on cell proliferation. A screen of > 5,800 deletion mutants, each heterozygous for a different protein-encoding gene in the S.cerevisiae genome, revealed that over 18% of these heterozygotes displayed a significantly reduced growth rate . This haploinsufficient phenotype was displayed even under conditions where there are no external constraints (such as nutrient limitations) on cell growth . For a smaller set (ca. 600 genes, listed in Additional file 1: Table S1) of ‘haploproficient’ genes, however, heterozygous deletion of the gene elicits significantly faster growth than the wild type. Using these yeast phenotypes, we have previously made correct predictions of a gene-dosage-related phenotype for their orthologous human genes , and verified these predictions by controlled RNAi knockdown in human cell lines .
The existence of haploproficient genes in the yeast genome indicates that the organism has not evolved to maximize its rate of growth, even when sugars became abundantly available with the emergence of flowering plants approximately 80–100 million years ago . The persistence of these genes in the yeast genome therefore suggests that there must be some major selective advantage that outweighs their growth-rate disadvantages. We demonstrate, here, that this advantage could be the maintenance of genome integrity, which is compromised when the dosage of these genes is reduced. For HP yeast genes, the altered gene dosage not only increases growth rate but, as we will show, copy-number reduction, as opposed to complete gene deletion, is sufficient to result in abnormal progression through the cell cycle, increased accumulation of DNA damage, and altered rates of apoptosis. This set of phenotypes is strongly reminiscent of cancer in mammalian cells. These results complement the recent conclusions from a study of aneuploid yeast strains  that the detriment to genome stability is driven by gene-dosage effects (e.g. stoichiometric imbalances), rather than simply by the presence of extra DNA (or additional centromeres; ). This emphasises the value of using model organisms to predict which human genes may impact on cancer in a dosage-dependent manner.
The screening of a library of yeast heterozygous deletion mutants for haploinsufficient or -proficient phenotypes is a high-throughput approach to determine what effects quantitative changes in the concentrations of gene products have on phenotype. A related approach is the search for drug-induced haploinsufficiency – in which heterozygous deletion strains exhibit altered sensitivity to a compound as a result of the decrease in the dosage of the target gene. In a pioneering study,  exposed a pool of 233 heterozygous mutants to sub-lethal compound concentrations, and later work has successfully elucidated the mode-of-action of novel compounds such as the anti-tumour agent dihydromotuporamine C , demonstrating the utility of such genome-wide yeast screens. In the light of our findings on haploproficiency in yeast, we combined both approaches and carried out a screen of anti-cancer agents against a set of S. cerevisiae mutants heterozygous for HP genes involved in the DNA damage response-pathway in order to search for altered sensitivities relative to both the WT and the corresponding homozygous (null) deletion mutant. This screen might inform both the appropriate treatment of tumour cells that carry CNVs of the candidate cancer-related genes, and also suggest novel combinations of specific inhibitors which may prove more effect than either drug in isolation.
Yeast haploproficient genes are involved in the maintenance of genome integrity and are orthologs of human cancer genes
The existence of haploproficient genes, and the inference that the yeast genome has not been optimised for maximal growth rate, does not appear to be an accident, nor unique to S. cerevisiae. By examining orthology relationships across the Ascomycetes, we find that haploproficient genes are more highly conserved than the genome average across the lineage (Fischer’s exact test; p < 0.01) (Table 1a). Thus, selective pressure has existed toward the retention of HP genes for several hundred million years (the evolutionary distance between S. cerevisiae and Sz. pombe), including through a period of strong selective pressure toward maximizing growth rate that occurred at the time of the whole-genome duplication in the Saccharomyces lineage (approximately 100 million years ago ). Congruent with our hypothesis of a trade-off between genome stability and growth rate optimisation, we find that haploproficient genes are overrepresented amongst those involved in the maintenance of genome integrity. A Gene Ontology term-enrichment revealed that the HP set is enriched for genes involved in the mitotic cell cycle (p = 0.003), and, in particular, the response to and repair of DNA damage (p = 0.006) (Figure 1; Additional file 2: Table S2). Given their integral role in maintaining genome stability, it is unsurprising that yeast HP genes are very much more likely to be orthologous to cancer genes than the S. cerevisaie genome average (p < 10-10).
We selected the 30 HP genes (Table 2) involved in the processes of DNA damage repair and sister chromatid segregation as the most relevant candidates in which to examine the relationship between varying gene dosage and cancer-related phenotypes. This ‘HP genome integrity’ (HPGI) set is even more highly conserved than the HP set as a whole (Table 1b); and more likely (than the HP set as a whole) to be orthologous to a cancer gene (p < 10-4). Nineteen genes in the set have a unique human ortholog (Table 2) and, of these, 12 (UBX4, IDP1, IDP2, MSH2, RAD1, TOP2, NBP2, MUS81, RAD54, DBF2, STP22, PBS2) are orthologous to cancer genes as annotated in the Cancer Gene Index (CGI ). Table 2 lists the cancer-specific OMIM disease associations of the orthologs of members of the HPGI set.
Orthologs of haploproficient genes exhibit CNV in tumour cells
The haploproficiency phenotype is, by definition, linked to a reduction in gene copy number. Our hypothesis posits that it is copy number variation (specifically, a reduction in gene copy number) of the orthologs of these genes that is relevant to human cancer. To determine the rate of incidence of CNV in orthologs of our HP cell cycle set, we queried our list against the NCI’s Cancer Genome Atlas database (TCGA ). No less than 17/19 of the human orthologs of the HPGI set have a copy number log2 ratio of magnitude >0.5 across eight types of cancer. In particular, 12/19 have a copy number reduction (i.e. a log2 ratio of < −0.5) in >25% of patients in one or more of the eight cancer types. This is a significant overrepresentation compared with 7750/~18500 of all genes in TCGA (p = 0.07), and with the set of non-HP cell-cycle/DNA repair orthologs (p = 0.05). For serous cystadenocarcinoma, kidney renal clear cell, and lung squamous cell carcinomas, there is a significant overrepresentation of orthologs of the HPGI set amongst genes exhibiting frequent copy-number reduction (p-values of 2 × 10-4, 10-4 and 10-3 respectively). Moreover, across all eight cancer types, orthologs of the HPGI set are overrepresented with a p-value of 0.003 (all corrected for multiple testing).
CNV of haploproficient genes is sufficient to elicit cancer-like phenotypic responses in yeast
Cell cycle phasing
As their growth rate is higher than the previously observed maximum, heterozygous deletion mutants of HP genes must a priori progress faster through the cell cycle. Either progress through all the phases of the cycle may be accelerated, or one or more phases must be relatively shorter than in a diploid cell with two copies of the gene. Since the products of HPGI genes are involved in checkpoint controls of the cell cycle, we reasoned that the faster cycle time in the heterozygotes could be the result of cells skipping a compromised checkpoint and therefore progressing faster through that phase of the cycle. This would manifest itself in an altered population distribution between the different cell cycle phases. This same cell cycle dysregulation, arising from compromised tumour suppressor gene function, is a mechanism of oncogenesis in mammalian cells . Previously, we found strong support for the hypothesis that changes in the copy number of the high flux control (HFC) genes alter cell cycle progression in a combined modelling/experimental study of a set of genes involved in the G2/M transition in yeast . This intrinsic link between haploproficiency and cell cycling is also borne out in our finding that copy-number variation of HP genes has no significant effect on yeast chronological lifespan; that is, survival in the extended non-cycling G0 state of stationary phase (see Additional file 3: Table S3).
Using flow cytometry, we analysed the cell cycle progression of heterozygous deletion mutants of the HPGI set, comparing them with the cell cycle profiles of the WT and of the non-cell cycle, non-HP controls HIS3/his3 and HO/ho, non-HP, cell-cycle HSL1/hsl1, CLB2/clb2, CLB5/clb5, CLB6/clb6, CLN2/cln2 and CLN3/cln3 and the non-cell cycle genes PNP1, MET7, HRK1 and TPO3. To distinguish between the effects of the complete absence of a given gene from those of reducing its copy number from two to one, we also compared the cycle profiles of the heterozygous deletant against that of the diploid homozygous deletants of the same gene. Complete cell cycle profile data is given in Additional file 4: Table S4.
We find that correct dosage of the HPGI genes is essential for normal cell cycling: heterozygous deletion is indeed sufficient to significantly alter the cell cycle profile relative to the WT/non-cell cycle control genes for 13 of the 30 HPGI genes (summarised in Table 3). Typically, the cell cycle perturbation for the heterozygote concurs with that previously reported for haploid null deletants of the gene (as annotated in SGD ), however BUB2 heterozygous deletion confers the opposite phenotype (an decrease in the relative G1 population) to that of both the null diploid and haploid.
In mammalian cells, compromising the link between the DNA damage response and apoptotic pathways can diminish the apoptotic response , which is a necessary step en route to cancer . Therefore, we carried out tests to determine whether compromising the DNA damage response in yeast by heterozygous deletion of HPGI genes affected the rate of apoptosis (see  for a review of S.cerevisiae programmed cell death). The degree of apoptosis occurring in the deletion strain populations, in response to treatment with methyl methanesulfonate (MMS) or tert-butyl hydroperoxide (TBHP). was measured by simultaneous annexin V (cell surface phosphatidylserine ) and propidium iodide (cell viability) staining  to distinguish between apoptotic and necrotic cells. For 15 of the 30 HPGI genes, the heterozygous deletion mutant exhibited a degree of apoptosis significantly different (p < 0.05) from than the WT – that is, partial knockdown of the gene expression is sufficient to disrupt the normal apoptotic response of the cell. Whilst exploring the specific mechanisms behind this disruption for each gene is beyond the scope of this paper, it is clear that deviating from the WT dosage of these particular genes has a significant effect on programmed cell death in response to genotoxic agents. Phenotypes for the mutants are described in Table 4; apoptosis rates in HIS3/his3Δ and HO/hoΔ control strains are comparable to WT.
The phenotypes observed for the HPGI heterozygous deletants were compared with those reported in the literature for the RNAi-mediated knockdown or pharmacological inhibition of their mammalian orthologs. The congruence between the yeast and mammalian phenotypes is summarized in Table 4. For 2/4 cases of increased apoptosis upon copy-number reduction, the phenotype is also observed in human cells, however for MLH1/MLH1 and UBX4/ASPSCR1 the relationship between gene dosage and apoptosis in human cells is acknowledged, but unclear .
Similarly, for 4/11 genes prompting reduced apoptosis upon copy-number reduction, knockdown of the human ortholog has been reported to cause a similar phenotype; we could find no report for the orthologs of a further 5 of the 11. For SMC3/SMC3 and DNL4/LIG4, however, copy-number reduction in yeast reduced apoptosis, whilst knockdown in mammalian systems has been reported to increase its occurrence [28, 29]. However, both of these reports involve a complete deletion of the gene, rather than the heterozygous deletion in which we observed the yeast phenotype. This suggests that further investigation in human cells of the effects of varying gene dosage of these genes, in particular, would be worthwhile.
Haploproficiency & cancer drug sensitivity
Given the strong connection that we have observed between yeast haploproficiency and human cancer, it is unsurprising that the human orthologs of many of the HPGI genes are the targets of various anti-cancer compounds (both approved drugs and those in late-stage clinical trials; see Table 5). However, in the light of the increased growth upon reduction of HP gene dosage, and the dosage-specific phenotypes reported above, it is possible that inhibitor treatment of a tumour cell could elicit the opposite to the desired response – i.e. increased proliferation rather than cell death – if complete ablation of the HP target function is not achieved. Therefore, we examined the drug sensitivity of wild-type yeast, and the heterozygous and homozygous deletion mutants for each of the non-essential HPGI genes. We also included the deletion mutants for an additional five yeast genes (HRK1, TPO3, MET7, PNP1 and GPD1), which are HP and whose products are orthologous to specific cancer-drug targets.
Where the human ortholog of a yeast HPGI gene product is a cancer-drug target (Table 5) we included in our screen either the commercial drug (where available) or alternative inhibitors (for example, PD98059 for Pbs2p). In total, 18 drugs were screened (see Table 5): two of these were specific alternative inhibitors; 15 were compounds either approved for human cancer treatment or shown to be effective in cell culture; and one control treatment of clotrimazole, a fungicide not known to have anti-cancer properties.
Drug sensitivity profiles were clustered (Figure 2 left panel) by agglomerative hierarchical clustering . The profile elicited by a compound can reveal its mode of action, and thus similarities between drug profiles may indicate the targeting of the same pathways in yeast. Reassuringly, known modes of action are reflected in our profiles – for example, the sensitivity of cell wall integrity/morphogenesis deletion mutants to phleomycin (Figure 2 left panel), which causes cell wall lesions .
Control (non-cancer drug) clotrimazole treatments are reasonably distinct from the cancer drug treatments. Aminopterin and methotrexate (which share a common, antifolate, mechanism of action) PD98059, cycloheximide and mitoxantrone cluster together, driven largely by the resistant phenotypes of deletion mutants of genes involved in chromosome condensation and segregation (SMC2, TOP1, RAD61, IRR1, MAD1). A second cluster is formed by Bay11, cantharidin and vorinostat (SAHA), all of which act as inhibitors of the NF-κB pathway in mammalian cells [35–37]. This pathway is absent in S.cerevisiae however, the clustering of the compounds suggests that their modes of action, though distinct from the mammalian-cell pathway, may also be related in yeast. rad54/rad54 mutants are particularly resistant to all three drugs, where, in general, these mutants are sensitive to all other treatments. Vorinostat has been demonstrated to induce DSBs in acute myeloid leukemia cells , suggesting that the DSB-repair function of Rad54p could potentially be a target. Indeed, the RAD54 ortholog, RAD54L, is strongly down-regulated in LNCAP (prostate adenocarcinoma) cells treated with vorinostat . This is also the case for the mammalian orthologs of CHK1 and TOP2, which are also resistant (though less strongly so) to the vorinostat /Bay11/cantharidin cluster. We have since demonstrated  that the magnitude of these drug-response phenotypes in yeast (deviations of up to ±20% of WT growth rate) is sufficient to translate into observable phenotypes in tumour cell lines.
Targeting the CNV profile of tumour cells
Where a copy of a gene is frequently lost in tumour cells, as is the case for orthologs of the HPGI set, the phenotype of the heterozygous deletion mutant of the yeast ortholog might better reflect the tumour cell’s response to drug treatment (Figure 3b). For a significant number of homozygous/heterozygous deletant pairs (9/30, p = 0.01) the drug sensitivity phenotypes are quantitatively dissimilar (by Euclidean squared distance, i.e. diverging at the top level of branching) (Figures 2 left and right panel). For these genes, therefore, gene dosage may be highly relevant to pharmaceutical efficacy. These eight genes (CCR4, TOP1, IDP1, RAD54, BUB2, MUS81, PNP1, and MAD1) have, on average, a greater number of protein-protein interactors (as annotated in BioGrid ) than the average for our HPGI set as a whole (180 unique interactors versus 135). This suggests that gene dosage balance may be an important contributor to phenotype for genes which are more central in interaction networks. We have explored the relationship between gene copy number and drug response further in .
Previously, we reported the striking result that treatment of wild-type yeast with low concentrations of inhibitors targeting HP genes can induce the same increased proliferative phenotype as is observed upon reducing the copy number of the HP target, a phenomenon we termed hypertolerance. We observe this phenotype again for treatment with cantharidin, which inhibits the PP2A complex, the subunits of which are strongly haploproficient (Additional file 5: Table S5), but not for any of the general cytotoxic compounds in the drug screen, reinforcing our previously-established link between the specific inhibition of a haploproficient target and drug hypertolerance. A number of heterozygous strains also exhibited hypertolerance in solid-media screens, which was subsequently confirmed by titrating across a range of drug concentrations in liquid medium (Table 5). For tumour cells bearing the orthologous CNVs, this would suggest a contraindication for treatment with the particular drug. In particular, the NBP2/nbp2 heterozygote is multiply drug-hypertolerant, of note since its ortholog SH3GL1 frequently has reduced copy-number in serous cystadenocarcinoma.
Conversely, for PNP1, the heterozygotes are mitoxantrone/methotrexate/aminopterin and vorinostat/Bay11/cantharidin sensitive, and the homozygotes resistant. This phenotype may be a instance of ‘obligate haploinsufficiency’ , whereby fitness is compromised as the gene dosage is reduced below some threshold value, but the phenotype is subsequently rescued through the switching on of a compensatory pathway.
The data presented here for the S.cerevisiae HPGI set point to the striking result that copy-number variation (in this case, deletion of just one gene copy from a diploid cell), rather than complete gene loss, is sufficient to elicit deleterious phenotypes, in particular those reminiscent of cancer in mammalian cells. This concurs with the increasing appreciation of the significance of copy-number variation to the genesis and progression of disease. In particular, there is a general lack of significant correlation of phenotypes between the heterozygous and homozygous (null) deletion mutants of a given gene. Nor are the phenotypes of the null mutants necessarily more extreme than those of the heterozygous deletants, as might naively be expected from a linear ‘dosage hypothesis’ . This emphasises the need to systematically investigate the phenotypic impact of gene dosage, rather than relying on knockout, or total-knockdown, studies.
A mechanism by which heterozygous deletion of a gene could be just as disruptive as a complete knockout is via the disruption of the stoichiometric ratios of the sub-units of protein complexes. This has been proposed as a mechanism for haploinsufficiency in the 'balance hypothesis' of Papp et al.[44–46]. It also suggests that dosage compensation, in which the impact of the heterozygous deletion of a gene is mitigated by the increased expression of the remaining copy, does not play a significant role amongst our HPGI gene set. Indeed,  showed that such dosage compensation is, in general, rare in S.cerevisae, and very few HP genes are amongst those exhibiting significant compensation.
For cell cycle phasing, heterozygous gene deletion is often sufficient to perturb the cycle from the WT profile, which indicates that cell cycle proteins are required in yeast at greater than 50% of WT dosage (the quantitative relationship between gene copy number and cell cycling is further explored in ). Similarly, heterozygous deletion of the yeast ortholog is, in general, both sufficient to alter the degree of apoptosis occurring in response to DNA-damaging agents (for 15 out of 30 HPGI genes), and to elicit the same apoptotic phenotype (in 8 out of 15 cases) as is reported for complete protein-product knockdown in mammalian cells. This general conservation of apoptotic phenotypes suggests that the DNA damage-related pathway(s) controlling apoptosis in S.cerevisiae are similar to those in mammals. This could be investigated further by undertaking a global survey of the effect of gene deletion on apoptosis rates in yeast, particularly since the current data on the degree of apoptosis in S.cerevisiae is limited (SGD, for example, reports altered apoptotic phenotypes for only 37 null/conditional mutants and 13 overexpression strains, none of which are in the HPGI set). In four cases, an altered apoptosis rate was observed in the yeast deletion mutants but has not, so far, been reported for the RNAi knock-downs of their human orthologs. We would suggest that, based on the apparent predictive value of the yeast phenotype, these genes should become the focus of an RNAi study in mammalian cells.
HP genes and the efficacy of anti-cancer drugs
This critical importance of considering gene dosage in the context of pharmaceutical intervention in cancer is further emphasised in the copy-number-dependence of drug sensitivity phenotypes that we observed with our yeast model. The mechanisms by which copy-number variation may exert a significant effect on phenotype are summarised in Figure 3.
Firstly, products of the mammalian orthologs of several of the yeast HPGI set are the targets of specific drugs used, or proposed for use, in treating cancers. Our data suggest that, in a majority of such cases, complete inhibition of the activity of a target protein product is necessary to achieve a positive therapeutic outcome. Incomplete inhibition, analogous to heterozygous deletion of the gene encoding the target, prompts increased proliferation or hypertolerance – the opposite of the intended anti-cancer effect. Furthermore, even if a given gene is not the intended specific target of a chemotherapeutic treatment, the modes-of-action, and secondary impacts, of many cancer drugs have not been fully elucidated. Drug-induced haploinsufficiency (or haplotolerance) data from yeast can contribute to a better understanding of both drug mechanisms and the functional conservation of drug-metabolism pathways between yeast and humans. For example, the clustering of mutant phenotypes in response to the mammalian-NF-κB inhibitors in this study suggests that all three compounds act through a common pathway in S.cerevisiae, which may be mediated by Rad54p.
Secondly, we observed that varying the copy number of the yeast ortholog can significantly alter the phenotypic response to drug treatment. This is especially for genes whose human orthologs have a high likelihood of CNV in tumour cells, In particular, several deletion strains are either more resistant than the WT, or themselves hypertolerant in response to a specific drug treatment. The impact of specific CNVs on drug sensitivity is becoming increasingly appreciated, and we believe our yeast mutant approach represents a high-throughput complement to the creation of drug-CNV profile ‘fingerprints’ for tumour cells (see e.g. ), and a model for determining the most effective drug interventions for tumours with a particular CNV profile.
Lastly, our data on the sensitivity/resistance of deletion mutants could potentially inform the design of combination therapies. Where a particular (heterozygous) deletion mutant is sensitive to a given drug, a combination therapy consisting of that drug plus an inhibitor of the product of the heterozygous gene could have a synergistic effect in reducing cell proliferation. Conversely, considering pairs of genes/drugs for which the deletion mutant is resistant to treatment could assist in identifying those drug combinations which are unlikely to succeed, reducing the search space involved in an otherwise expensive combinatorial problem .
Finally, the several instances in which we observed that the heterozygous deletion mutant is more sensitive to drug treatment than the homozygote provide evidence for the ‘obligate haploinsufficiency’ hypothesis of Berger et al.. Identifying such genes, and defining the protein product threshold below which their associated compensatory pathways are activated, could help to define the precise inhibitor dosage to achieve maximal therapeutic efficacy.
Haploproficient (HP) genes and are for which, when one copy is deleted from a diploid cell, the rate of cell proliferation is increased. We have demonstrated that, for a subset of yeast HP genes, heterozygous deletion causes aberrant cell cycling and altered rates of apoptosis, which are phenotypes associated with cancer in mammalian cells. A majority of these yeast genes are the orthologs of mammalian cancer genes, and hence our studies suggest that CNV of these oncogenic orthologs may be sufficient to lead to tumourigenesis in human cells. Moreover, where not already implicated, this cluster of cancer-like phenotypes in this model eukaryote may be predictive of the involvement in cancer of the mammalian orthologs of these yeast HP genes. Using the yeast set as a model, we have shown that the response of the heterozygotes to a range of anti-cancer drugs is strongly dependent on gene dosage, such that intermediate concentrations of the drugs can actually increase a mutant’s growth rate. This suggests such compounds may need to achieve near-total inhibition of target activity if they are to be a beneficial, rather than a deleterious, therapy.
Haploproficient genes and orthology analysis
The set of S.cerevisiae genes which are haploproficient in turbidostat culture was obtained using the growth data of  and an FDR cutoff of 0.02. This stringent FDR cut-off rigorously defines those genes for which heterozygosity confers a strong fitness advantage, but has no effect on the functional enrichment of genes identified as haploproficient. Genes defined as ‘haploproficient’ for the purposes of this study are listed in Additional file 1: Table S1. The set of chromosome maintenance-associated HP genes described in  overlaps, but is not coincident, with the HPGI set studied here, since the current set also includes DNA damage-response genes.
Orthology assignments were made using the InParanoid algorithm  and compared with the results of a BLAST  reciprocal best-hits search. GO enrichment searches were performed using the Babelomics 4 FatiGO tool . To assess the significance of HP gene conservation, the number of HP genes having orthologs in a given Ascomycete species, given the number of S. cerevisiae HP genes, was compared against the whole-genome conserved proportion using a χ2 or Fisher exact test (depending on sample size), with the null hypothesis of identical distribution. All findings of significance were reiterated using a Z test for difference of proportions. Where necessary, P values were corrected for multiple testing using the Bonferroni correction. Cell cycle and DNA damage repair pathways were obtained from the KEGG pathway database .
Expression data for S.cerevisae genes was obtained from the Saccharomyces Genome Database ; and protein expression levels from . A list of human cancer genes/oncogenes was obtained from the Cancer Gene Index ; enrichment of HP genes amongst the orthologs was determined using a χ2 test as above. CNV incidence across eight tumour types (breast invasive carcinoma, rectum adencarcinoma colon adenocarcinoma, kidney renal cell clear carcinoma, uterine corpus endometrioid carcinoma, glioblastoma multiforme, acute myeloid leukemia, lung adenocarcinoma, lung squamous cell carcinoma, serous cystadenocarcinoma) as measured by comparative genomic hybridisation, was obtained from the NCI Cancer Genome Atlas online data browser  with a copy number (log2 ratio) of magnitude >0.5 taken as the significance threshold. Details of the sampling and analysis of the tumour samples are described in . A P-value for HP ortholog overrepresentation was calculated using a χ2 test .The TGCA database was also used to perform a pathway search for overrepresentation of HP orthologs.
In total, 30 HP genes were chosen for analysis, based upon the criteria discussed in the Results above. The heterozygous deletion mutant of each gene was obtained from the heterozygous diploid deletion library (Open Biosystems), in the BY4743 (MAT a /α, his3D1/his3D1, leu2D0/leu2D0, LYS2/lys2D0, met15D0/MET15, ura3D0/ura3D0) genetic background. For non-essential genes, the homozygous deletant was retrieved from the analogous homozygous diploid deletion library (Open Biosystems).
Control strains were the BY4743 WT, along with the heterozygous deletion mutant of the non-functional his3 locus; the non-HP, non-cell cycle ho/HO heterozygous deletion strain; and the heterozygous deletion mutant of the non-HP, cell cycle gene HSL1. In addition, heterozygous deletion mutants of the G1 and G2 cyclins were included in several of the experiments. A complete list of the strains used is provided in Additional file 6: Table S6.
Flow cytometric analysis of the deletion strains’ cell cycle profiles was carried about following the method of . Briefly, ~107 cells in mid-exponential phase were harvested, washed, and fixed in absolute ethanol at 4C overnight. Fixed cells were then collected, washed, and boiled for 15 minutes in 2 mg/mL RNAse in 50 mM Tris-Cl (pH 8), and incubated at 37C for 2–12 hours. Cells were resuspended in protease solution (5 mg/mL pepsin, 4.5 μL/mL concentrated HCl), incubated for 15 minutes at 37C and resuspended in 50 mM Tris (pH 7.5). For analysis, 50 mL of cell suspension was added to 1 mL of 1 mM Sytox Green in 50 mM Tris pH 7.5), vortexed and analysed using a Cyan flow cytometer (Beckman Coulter). FlowJo (Tree Star) analysis software was used to fit histograms to the peaks representing 1C and 2C DNA content, and thereby calculate the number of cells in the G1 and G2 phases, and infer the number in S phase from the remaining fraction of the population.
Chronological lifespan assay
Cultures were inoculated from frozen stocks, grown overnight in YPD at 3°C, and 200mL of each was transferred into a well of a 96-well microtiter plate (Corning). Strains were present in duplicate on each plate, with a buffer of WT in the wells around the edge of the plate, so edge effects would not impact test colony measurements. A Singer Rotor HDA colony pinning robot was used to spot four replicates of each well onto a YPD + 10 μg/mL phloxine B (Sigma) plate. Phloxine B is a fluorescein derivative taken up when the cell membrane is disrupted upon cell death . Plates were incubated for 48 hours at 3°C and photographed using an Epson 1240 Scanner. The colony images were analysed using a custom image-analysis code written in MatLab, with colony size measured by pixel count, and fraction of dead cells by the intensity of colony redness . Since these parameters are independent, this allowed the dissection of the effect of cell viability upon colony growth from that of growth rate variation. The 96-well liquid cultures were incubated at 3°C, and, every second day over a period of three weeks, the colony-pinning onto YPD + phloxine B and image analysis repeated. For each plate, the median culture intensity for each strain was compared with the growth of the WT on that plate, and also with the strain growth and viability after the initial 48-hour period. The experiment was performed twice.
At several points throughout the 3-week period, several strains were selected at random, and viability assayed by performing serial dilutions and counting colony-forming units. These results were checked for compatibility with the microplate viability results.
The rate of occurrence of apoptosis in the different strain populations was measured in two ways. Apoptosis was first induced by pretreating cells with 0.001%, 0.01% MMS, 0.0001% or 0.001% TBHP in overnight culture; keeping a negative, non-induced WT control sample.
The translocation of phosphatidyl serine to the cell surface, a marker of apoptosis , was measured using an Annexin V-FITC Apoptosis Detection kit. (Sigma). Cells were harvested, washed in 1.2M sorbitol, 0.5 mM MgCl2, 35 mM K phosphate (pH 6.8) and then digested in 5.5% glusulase (Sigma) and 15 U/mL lyticase (Sigma) for 2 hours at 28C. Spheroplasts were harvested, washed in binding buffer (10 mM Hepes/NaOH pH 7.4, 140 mM NaCl, 2.5 mM CaCl2 in 1.2 M sorbitol buffer) and resuspended in binding buffer/sorbitol. 5 mL of FITC-labelled annexin V, and 10 mL of 10010 mg/mL propidium iodide were added to each sample, with control samples containing 1.) no label, 2.) FITC-annexin V only, and 3.) PI only. Fluorescence was quantified using a CyAn (Beckman Coulter). Gates were fitted on the basis of the the control samples, dividing a log PI versus log FITC plot into four quadrants: lower left (neither FITC nor PI-stained) – viable cells; upper left (PI stain only) – necrotic cells; lower right (FITC only) – early apoptotic cells; and upper right (PI and FITC-stained) – late apoptotic cells. FlowJo software (TreeStar) was used to count the fraction of the total cell population in each quadrant. The proportion of both necrotic and apoptotic cells for each strain was normalised to strain viability (i.e. on the basis of the proportion of cells assigned to the lower-left FITC/PI quadrant), and the ratio of necrotic:apoptotic cells calculated. Ratios for each strain were normalised to the WT value, and the standard deviation across all samples calculated. Strains having a necrosis:apoptosis ratio further than 1.5x this standard deviation from WT levels were deemed to demonstrate abnormal apoptosis rates.
Growth rate and drug sensitivity assays
Growth and drug sensitivity assays were performed both on solid media and in liquid cultures. For solid assays, the required drug concentration was added to YPD-agar containing 10μg/m/mL phloxine B. Overnight cultures of the strains were spotted onto the (drug-containing) plates using a Singer rotor, as above. Plates were incubated at 3°C and photographed at 24 and 48 hours and analysed using an image-processing code as described above. Strain growth and viability was compared both with WT growth on the same plate, and with growth on YPD-agar (or YPD-agar plus DMSO, where the drug is DMSO-soluble). The ratio of viability and size with and without drug was calculated for every strain on a plate, and the standard deviation of all ratios calculated. Strains having a drug:untreated ratio greater than or less than two standard deviations from that of the WT were deemed to be resistant and sensitive, respectively.
Assays in liquid culture were performed by transferring 5mL of overnight culture into each well of a 96-well microtitre plate, containing 200 μL of YPD plus the required concentration of drug. Absorbance was measured for 30 hours at 3°C using a BMG Optima platereader, maximum growth rate calculated using a curve-fitting script written in R, and the growth rate for each strain compared with that of the WT in the same plate, and growth in YPD/YPD + DMSO.
Copy number variation
Haploproficient genome integrity
Saccharomyces Genome Database.
Craddock N: Genome-wide association study of CNVs in 16,000 cases of eight common diseases and 3,000 shared controls. Nature. 2010, 464: 713-20. 10.1038/nature08979.
Speleman F, Kumps C, Buysse K, Poppe B, Menten B, De Preter K: Copy number alterations and copy number variations in cancer: close encounters of the bad kind. Cytogenet Genome Res. 2008, 123: 176-82. 10.1159/000184706.
Shlien A, Malkin D: Copy number variations and cancer susceptibility. Curr Opin Oncol. 2010, 22: 55-63. 10.1097/CCO.0b013e328333dca4.
Weaver BA, Silk AD, Montagna C, Verdier-Pinard P, Cleveland DW: Aneuploidy acts both oncogenically and as a tumor suppressor. Cancer Cell. 2007, 11: 25-36. 10.1016/j.ccr.2006.12.003.
Wilkie AO: The molecular basis of genetic dominance. J Med Genet. 1994, 31: 89-98. 10.1136/jmg.31.2.89.
Veitia RA, Birchler JA: Dominance and gene dosage balance in health and disease: why levels matter!. J Pathol. 2010, 220: 174-85.
Delneri D, Hoyle DC, Gkargkas K, Cross EJ, Rash B, Zeef L, Leong HS, Davey HM, Hayes A, Kell DB, Griffith GW, Oliver SG: Identification and characterisation of high-flux-control genes of yeast through competition analyses in continuous cultures. Nat Genet. 2008, 40: 113-7. 10.1038/ng.2007.49.
Pir P, Gutteridge A, Wu J, Rash B, Kell DB, Zhang N, Oliver SG: The genetic control of growth rate: a systems biology study with yeast. BMC Syst Biol. 2012, 6: 4-10.1186/1752-0509-6-4.
de Clare M, Pir P, Oliver SG: Haploinsufficiency and the sex chromosomes from yeasts to humans. BMC Biol. 2011, 9: 15-10.1186/1741-7007-9-15.
Freeland MC: The Impact of Gene Copy Number on the Control of Cell Growth Rate from Yeast to Humans. 2012, Cambridge: (PhD Thesis) University of Cambridge
Piskur J, Rozpedowska E, Polakova S, Merico A, Compagno C: How did Saccharomyces evolve to become a good brewer?. Trends Genet. 2006, 22: 183-6. 10.1016/j.tig.2006.02.002.
Scheltzer JM, Blank HM, Pfau SJ, Tange Y, George BM, Humpton TJ, Brito IL, Hiraoka Y, Niwa O, Amon A: Aneuploidy drives genomic instability in yeast. Science. 2011, 333: 1026-30. 10.1126/science.1206412.
Futcher B, Carbon J: Toxic effects of excess cloned centromeres. Mol Cell Biol. 1986, 6: 2213-22.
Giaever G, Shoemaker DD, Jones TW, Liang H, Winzeler EA, Astromoff A, Davis RW: Genomic profiling of drug sensitivities via induced haploinsufficiency. Nat Genet. 1999, 21: 278-83. 10.1038/6791.
Baetz K, McHardy L, Gable K, Tarling T, Reberioux D, Bryan J, Andersen RJ, Dunn T, Hieter P, Roberge M: Yeast genome-wide drug-induced haploinsufficiency screens to determine drug mode of action. Proc Natl Acad Sci USA. 2004, 101: 4525-30. 10.1073/pnas.0307122101.
Scannell DR, Frank AC, Conant GC, Byrne KP, Woolfit M, Wolfe KH: Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication. Proc Nat Acad Sci USA. 2007, 104: 8397-402. 10.1073/pnas.0608218104.
Cancer Gene Index. http://www.sophicalliance.com/,
Collins JA, Schandi CA, Young KK, Vesely J, Willingham MC: Major DNA fragmentation is a late event in apoptosis. J Histochem Cytochem. 1997, 45: 923-34. 10.1177/002215549704500702.
Engel SR, Balakrishnan R, Binkley G, Christie KR, Costanzo MC, Dwight SS, Fisk DG, Hirschman JE, Hitz BC, Hong EL, Krieger CJ, Livstone MS, Miyasato SR, Nash R, Oughtred R, Park J, Skrzypek MS, Weng S, Wong ED, Dolinski K, Botstein D, Cherry JM: Saccharomyces Genome Database provides mutant phenotype data. Nucleic Acids Res. 2010, 38: D433-6. 10.1093/nar/gkp917.
Wang JYJ: DNA damage and apoptosis. Cell Death Differ. 2001, 8: 1047-1048. 10.1038/sj.cdd.4400938.
Hanahan D, Weinberg RA: The hallmarks of cancer. Cell. 2000, 100: 57-70. 10.1016/S0092-8674(00)81683-9.
Carmona-Gutierrez D, Eisenberg T, Büttner S, Meisinger C, Kroemer G, Madeo F: Apoptosis in yeast: triggers, pathways, subroutines. Cell Death Differ. 2010, 17: 763-73. 10.1038/cdd.2009.219.
Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C: A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995, 184: 39-51. 10.1016/0022-1759(95)00072-I.
Madeo F, Frohlich E, Frohlich KU: A yeast mutant showing diagnostic markers of early and late apoptosis. J Cell Biol. 1997, 139: 729-34. 10.1083/jcb.139.3.729.
Pabla N, Ma Z, McIlhatton MA, Fishel R, Dong Z: hMSH2 recruits ATR to DNA damage sites for activation during DNA damage-induced apoptosis. J Biol Chem. 2011, 286: 10411-8. 10.1074/jbc.M110.210989.
Ray RM, Bhattacharya S, Johnson LR: Protein phosphatase 2A regulates apoptosis in intestinal epithelial cells. J Biol Chem. 2005, 280: 31091-100. 10.1074/jbc.M503041200.
Sordet O, Goldman A, Redon C, Solier S, Rao VA, Pommier Y: Topoisomerase I requirement for death receptor-induced apoptotic nuclear fission. J Biol Chem. 2008, 283: 23200-8. 10.1074/jbc.M801146200.
Ghiselli G: SMC3 knockdown triggers genomic instability and p53-dependent apoptosis in human and zebrafish cells. Mol Cancer. 2006, 5: 52-
Eppig JT, Blake JA, Bult CJ, Kadin JA, Richardson JE, the Mouse Genome Database Group: The Mouse Genome Database (MGD): comprehensive resource for genetics and genomics of the laboratory mouse. Nucleic Acids Res. 2012, 40: D881-86. 10.1093/nar/gkr974.
Basu I, Cordovano G, Das I, Belbin TJ, Guha C, Schramm VL: A transition state analogue of 5’-methylthioadenosine phosphorylase induces apoptosis in head and neck cancers. J Biol Chem. 2007, 282: 21477-86. 10.1074/jbc.M702287200.
Wang Y, Decker SJ, Sebolt-Leopold J: Knockdown of Chk1, Wee1 and Myt1 by RNA interference abrogates G2 checkpoint and induces apoptosis. Cancer Biol Ther. 2004, 3: 305-13. 10.4161/cbt.3.3.697.
Jackson T, Ahmed MAH, Seth R, Jackson D, Ilyas M: MLH1 function is context dependent in colorectal cancers. J Clin Pathol. 2010, 62: 141-5.
Eisen MB, Spellman PT, Brown PO, Botstein D: Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA. 1998, 95: 14863-8. 10.1073/pnas.95.25.14863.
Moore CW, Del Valle R, McKoy J, Parmanik A, Gordon RE: Lesions and preferential initial localisation of [S-methyl-3H]bleomycin A2 on Saccharomyces cerevisiae cell walls and membranes. Antimicrob Agents Chemother. 1992, 36: 2497-505. 10.1128/AAC.36.11.2497.
Mori N, Yamada Y, Ikeda S, Yamasaki Y, Tsukasaki K, Tanaka Y, Tomonaga M, Yamamoto N, Fugii M: Bay 11–7082 inhibits transcription factor NF-kappaB and induces apoptosis of HTLV-I-infected T-cell lines and primary adult T-cell leukemia cells. Blood. 2002, 100: 1828-34. 10.1182/blood-2002-01-0151.
Miller SC, Huang R, Sakamuru S, Shukla SJ, Attene-Ramon MS, Shinn P, Van Leer D, Leister W, Austin CP, Xia M: Identification of known drugs that act as inhibitors of NF-kappaB signalling and their mechanism of action. Biochem Pharmacol. 2010, 79: 1272-80. 10.1016/j.bcp.2009.12.021.
Galimberti S, Canestraro M, Maffei R, Marasca R, Guerrini F, Piaggi S, Ciabatti E, Petrini M: Vorinostat interferes with Wnt and NF-kappaB pathways in the M-07e cell line. Leukemia. 2009, 23: 1935-8. 10.1038/leu.2009.119.
Srinivasan V, Kriete A, Sacan A, Jazwinski SM: Comparing the yeast retrograde response and NF-kappaB stress responses: implications for aging. Aging Cell. 2010, 9: 933-41. 10.1111/j.1474-9726.2010.00622.x.
Petruccelli LA, Dupere-Richer , Pettersson F, Retrouvey H, Skoulikas S, Miller WH: Vorinostat induces reactive oxygen species and DNA damage in acute myeloid leukemia cells. PLoS One. 2011, 6: e20987-10.1371/journal.pone.0020987.
Namdar M, Perez G, Ngo L, Marks PA: Selective inhibition of histone deacetylase 6 (HDAC6) induces DNA damage and sensitises transformed cells to anticancer agents. Proc Natl Acad Sci USA. 2010, 107: 20003-8. 10.1073/pnas.1013754107.
Stark C, Breitkreutz BJ, Reguly T, Boucher L, Breitkreutz A, Tyers M: Biogrid: A general repository for interaction datasets. Nucleic Acids Res. 2006, 34: D535-9. 10.1093/nar/gkj109.
Berger AH, Knudson AG, Pandolfi PP: A continuum model for tumour suppression. Nature. 2011, 476: 163-9. 10.1038/nature10275.
Kondrashov FA, Koonin EV: A common framework for understanding the origin of genetic dominance and evolutionary fates of gene duplications. Trends Genet. 2004, 20: 287-90. 10.1016/j.tig.2004.05.001.
Papp B, Pál C, Hurst LD: Dosage sensitivity and the evolution of gene families in yeast. Nature. 2003, 424: 194-7. 10.1038/nature01771.
Feeling M, Thomas BC: Gene-balanced duplications, like tetraploidy, provide predictable drive to increase morphological complexity. Genome Res. 2006, 16: 805-14. 10.1101/gr.3681406.
Birchler JA, Veitia RA: Gene balance hypothesis: connecting issues of dosage sensitivity across biological disciplines. Proc Natl Acad Sci USA. 2012, 109: 14746-53. 10.1073/pnas.1207726109.
Springer M, Weissman JS, Kirschner MW: A general lack of compensation for gene dosage in yeast. Mol Syst Biol. 2010, 6: 368-
Gamazon ER, Huang RS, Dolan ME, Cox NJ: Copy number polymorphisms and anticancer pharmacogenomics. Genome Biol. 2011, 12: R46-10.1186/gb-2011-12-5-r46.
Fitzgerald JB, Schoeberl B, Nielsen UB, Sorger PK: Systems biology and combination therapy in the quest for clinical efficacy. Nat Chem Biol. 2006, 2: 458-66. 10.1038/nchembio817.
O'Brien KP, Remm M, Sonnhammer ELL: Inparanoid: a comprehensive database of eukaryotic orthologs. Nucleic Acids Res. 2005, 33: D476-480.
Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden TL: NCBI BLAST: a better web interface. Nucleic Acids Res. 2008, 36: W5-W9. 10.1093/nar/gkn201.
Al-Shahrour F, Diaz-Uriarte R, Dopazo J: FatiGO: a web tool for finding significant associations of Gene Ontology terms with groups of genes. Bioinformatics. 2004, 20: 578-580. 10.1093/bioinformatics/btg455.
Kanehisa M, Goto S, Furumichi M, Tanabe M, Hirakawa M: KEGG for representation and analysis of molecular networks involving diseases and drugs. Nucleic Acids Res. 2011, 38: D355-60.
Ball CA, Jin H, Sherlock G, Weng S, Matese JC, Andrada R, Binkley G, Dolinski K, Dwight SS, Harris MA, Issel-Tarver L, Schroeder M, Botstein D, Cherry JM: Saccharomyces Genome Database provides tools to survey gene expression and functional analysis data. Nucleic Acids Res. 2001, 30: 69-72.
Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O’Shea EK, Weissman JS: Global analysis of protein expression in yeast. Nature. 2003, 425: 737-41. 10.1038/nature02046.
Haase SB, Reed SI: Improved flow cytometric analysis of the budding yeast cell cycle. Cell Cycle. 2002, 1: 132-6.
Nurse P, Thuriaux P, Nasmyth K: Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1976, 146: 167-78. 10.1007/BF00268085.
Fadok VA, Bratton DL, Frasch SC, Warner ML, Henson PM: The role of phosphatidylserine in recognition of apoptotic cells by phagocytes. Cell Death Differ. 1998, 5: 551-62. 10.1038/sj.cdd.4400404.
MdC was a Herchel Smith Scholar. This work was supported by awards to SGO from the Biotechnology & Biological Sciences Research Council (Grant BB/C505140/1) and the UNICELLSYS Collaborative Project (No. 201142) of the European Commission.
The authors declare that they have no competing interests.
MdC and SGO conceived the study together. MdC performed the experiments and analysed the data. Both authors wrote the paper. Both authors read and approved the final manuscript.
Electronic supplementary material
Additional file 2: Table S2: Haploproficient members of the major complexes within the S.cerevisiae cell cycle. (DOCX 44 KB)
Additional file 3: Table S3: Chronological lifespan data for heterozygous deletion mutants of the HPGI set compared with control strains. (XLSX 50 KB)
Additional file 4: Table S4: Cell cycle profiling data for the heterozygous and homozygous deletion mutants of the HPGI set, and control strains. (XLSX 50 KB)
Additional file 5: Table S5: Growth phenotypes of heterozygous deletion mutants of genes encoding subunits of the yeast PP2A complex. (DOCX 47 KB)
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de Clare, M., Oliver, S.G. Copy-number variation of cancer-gene orthologs is sufficient to induce cancer-like symptoms in Saccharomyces cerevisiae. BMC Biol 11, 24 (2013). https://doi.org/10.1186/1741-7007-11-24
- Copy-number variation
- Model organism