Thirty years on: HIV receptor gymnastics and the prevention of infection
BMC Biology volume 11, Article number: 57 (2013)
During 30 years of research on human immunodeficiency virus (HIV), our knowledge of its cellular receptors - CD4, CCR5 and CXCR4 - has illuminated aspects of the pathogenesis of the acquired immune deficiency syndrome (AIDS). Studying how the HIV envelope glycoproteins interact with the receptors led to anti-retroviral drugs based on blocking the docking or fusion of virus to the host cell. Genetic polymorphisms of CCR5 determine resistance to HIV infection and the rate of progression to AIDS. Eliciting neutralizing antibodies to the sites of receptor interaction on HIV glycoproteins is a promising approach to HIV vaccine development.
As we celebrate the 30th anniversary of the discovery of HIV-1 by Françoise Barré-Sinoussi and colleagues , it is a sobering thought that during the two years between the first notification of AIDS  and the discovery of its cause, epidemiologists established all the modes of transmission and risk factors associated with HIV-1 infection without knowing the identity of the virus. Moreover, it became apparent from the first full clinical description of AIDS in 1981  that the salient feature underlying the disease is a specific depletion of CD4 T-helper lymphocytes. So was the 1983 discovery of HIV-1 in Paris and its confirmation a year later by new isolations from AIDS patients in Africa , and America [5, 6] such a great leap forward? Most certainly yes!
First, the identification of HIV-1 rapidly led to diagnostic tests of infection that made blood donations safe again. Second, the recognition of HIV as a retrovirus led to the development of anti-retroviral drugs that have so dramatically reduced AIDS mortality and morbidity where treatment is available. Third, the measurement of virus load (the number of HIV genomes in the plasma) became an important prognostic marker, alongside CD4 T-cell counts, for monitoring the health of patients. Fourth, analyses of HIV informed us early on of ineffective immune responses to it [7, 8] and of the enormous antigenic diversity of the virus , which have been stumbling blocks in the development of a broadly efficacious vaccine.
HIV envelope glycoproteins
The 135 m diameter ferris wheel called the London Eye (Figure 1a) is an apt model of a cross-section of the HIV particle magnified by 109. The viral envelope is studded with viral glycoprotein ‘spikes’ that recognize the cell receptors to which HIV binds as the first step in virus entry. Each spike (Figure 1b) has a trimeric structure comprising three transmembrane glycoproteins (gp41) coupled to three surface glycoproteins (gp120) named after their approximate molecular weights of 41,000 and 120,000 Da, respectively. Gp120 is heavily glycosylated, particularly with N-linked high-mannose residues and the carbohydrates make up more than 50% of its mass . They form a glycan shield or carapace protecting sensitive sites such as receptor-binding pockets, but also present epitopes recognized by some neutralizing antibodies .
Figure 1 is deceptive, however, in that the envelope spikes are actually flexible structures that undergo important conformational rearrangement during binding and entry into cells . In order to enter cells, HIV must bind to CD4, the signature cell-surface marker of T-helper cells, and to one of the chemokine receptors characteristic of these cells. Gp120 bears the binding sites for the CD4 viral receptor and chemokine co-receptors, while gp41 contains the hydrophobic domains that effect fusion between viral envelope and host membranes.
The CD4 receptor and the shape of viral recognition
The discovery that CD4 is the HIV receptor followed soon after the description of HIV-1. As already mentioned, destruction of CD4-positive T-helper lymphocytes was highlighted in an early report  of the nature of the immunodeficiency, which was subsequently called AIDS. This selective depletion of CD4+ cells was replicated in vitro by David Klatzmann and colleagues, who sorted CD3+ T cells into CD4+ and CD8+ enriched fractions and observed that HIV replicated in and destroyed only the CD4+ population . These findings did not necessarily imply that the very same cell surface maker that immunologists use to type T-cell subsets would be recognized by HIV. However, that proved to be the case when monoclonal antibodies specific to CD4 were found to competitively block HIV infection in vitro[14, 15]. The binding epitope on CD4 was later mapped via monoclonal antibodies, site-specific mutagenesis and structural studies to be located on the amino-terminal domain, for which a phenylalanine residue at position 43 is crucial for binding in a gp120 pocket .
CD4 is a single chain class I membrane glycoprotein of the immunoglobulin superfamily. Five years after the recognition of CD4 as the HIV receptor, two other members of this family were identified as viral receptors, namely the adhesion molecule ICAM-1 for the major group of rhinoviruses  and a related molecule for all three serotypes of poliovirus . The binding sites for these receptors are buried in deep pockets or clefts in the viral proteins that bind them, as is the sialic acid binding site on the hemagglutinin of influenza viruses. This led Michael Rossman to postulate the canyon hypothesis  that viruses adopt this kind of receptor because bulky antibodies bearing both heavy and light chains at the antigen-binding sites cannot penetrate into the canyon on the virus. This consideration led us to exploit llama heavy-chain only antibody fragments, which should be able to gain access to the canyon in a similar way to CD4 itself. However, some potently neutralizing conventional human antibodies like VRC01 also recognize the CD4 binding site so penetration deep into the pocket may not be essential .
Chemokine coreceptors and resistance to HIV
As well as infecting T-helper lymphocytes, HIV can infect macrophages and other cells of the same lineage, such as microglia in the brain, which express low levels of CD4. It soon became clear, however, that CD4 was necessary but not sufficient for HIV infection, as expression of human CD4 on murine cells did not confer susceptibility to HIV entry . It took a further ten years before seven-transmembrane chemokine receptors were identified as the missing component or co-receptor, with a landmark paper  on CXCR4 serving as the co-receptor for cell-line adapted strains of HIV-1. Three months before the discovery of CXCR4 as a co-receptor for HIV, it had been reported that CCL3L1 (MIP-1αP), CCL4 (MIP-1β) and CCL5 (RANTES) could block infection . The only receptor that binds all three of these chemokines is CCR5. With this clue and the discovery of CXCR4, several groups quickly demonstrated that CCR5 is the co-receptor for HIV strains that infect primary T cells and macrophages .
CCR5-using (R5) viruses represent the major transmissible HIV-1 strains, whereas CXCR4-using (X4) viruses tend to arise late in the course of disease. X4 viruses are often thought to precipitate AIDS, but they occur only in a minority of AIDS patients. Turning the argument on its head, we suggested that while X4 viruses are ill-adapted for propagation in healthy individuals, they emerge as opportunistic HIV variants once immunodeficiency begins to set in .
The discovery of CCR5 as a co-receptor rapidly led to the identification of a major genetic resistance factor for HIV infection. It had been a puzzle that some highly exposed sexual partners of HIV-infected individuals had managed to escape infection. It was shown that one such patient was a natural CCR5 knock-out, being homozygous for a deletion of 32 base pairs in exon 1 of the CCR5 gene (Δ32), and his cells could not be infected in vitro. The Δ32 allele occurs relatively frequently in Caucasian populations and while heterozygotes are not wholly resistant to HIV, they are less susceptible to infection, and once infected, they progress to AIDS at a slower rate than people with wild-type CCR5. An HIV-infected individual with leukemia who received a bone marrow stem cell transplant from a CCR5-negative donor appears to have slowly eliminated his virus . Other CCR5 polymorphisms in the promoter region also occur in non-Caucasian populations and affect susceptibility to HIV and progression to AIDS . The density of expression of CCR5 on lymphocytes and the plasma concentration of chemokines that bind to it act in concert to modulate efficiency of HIV entry.
A number of other chemokine receptors can act as functional co-receptors for HIV in vitro but there is scant evidence that they play a role in vivo. Of more practical significance is the discovery that HIV binds to the adhesion molecule DC-SIGN on dendritic cells . DC-SIGN does not serve as a receptor for virus entry, but dendritic cells migrating from mucosal tissues to lymph nodes and bearing HIV particles on the surface provide a route whereby the virus can be delivered to susceptible CD4+ CCR5+ T cells in the lymph nodes.
The gymnastics of fusion and entry of virus into cells
HIV entry involves a stepwise series of interactions with receptors that initiate conformational changes in the envelope glycoproteins [12, 27] (Figure 2). Docking on to CD4 induces a conformational change in gp120 that exposes a site known as the CD4 induced site (CD4i) and allows the protrusion of one of three hypervariable loops of gp120 (variable loop 3, or V3 loop). Both CD4i and the V3 loop interact with chemokine receptors, the V3 loop being the major determinant of R5 and X4 tropism. At the same time a hinge region between globular domains 2 and 3 on CD4 bends to move the HIV envelope trimer closer to CCR5. In turn, a loosening of gp120 allows gp41 to undergo a radical rearrangement that induces the formation of a hydrophobic coiled-coil or six-helix bundle that initiates fusion between viral envelope and cell membrane.
One unanswered question is why the fusion reaction of envelope and cell membrane takes place within endocytotic vesicles (at least in HeLa cell derivatives), since it seems that it is not dependent on low pH . Since X4 viruses readily induce cell-cell fusion, it may well be that these viruses fuse virus and host membranes at the cell surface, whereas R5 viruses may be restricted to undergo fusion in endosomes. A recent paper  shows that cortical actin is involved in the pre-fusion conformational changes downstream of gp120-induced signaling via CD4, which promotes HIV entry; abnormally high - or low - levels of gelsolin (which severs cortical actin) inhibit HIV infection.
Much of the spread of HIV infection within an infected person takes place through close contact between cells whereby infected cells form a ‘virological synapse’ with target immune cells . The synapse is held together by adhesion molecules, as well as requiring CD4 and CCR5, and HIV particles move across it from one cell to another. Infected cells can thus spread infection by migrating and disseminating the virus through synaptic contact within the lymph nodes and gut lymphoid tissue where HIV is most likely to encounter activated T cells, in which it replicates best .
Targeting HIV entry in treatment and prevention
Early steps in HIV infection, before the virus has entered cells, are amenable to drugs that are aimed at preventing entry and need not penetrate into the cytoplasm or nucleus . The first potential drug was a soluble form of the CD4 molecule itself that potently neutralized X4 strains, but was only weakly active against R5 strains. However, replacing the head of the heavy chain of IgG with the two amino-terminal domains of CD4 yielded a bivalent protein with more potent anti-HIV activity against both types of virus.
Enfurtide is a 20 amino acid peptide that mimics the fusigenic formation of gp41 sequence and blocks formation of the six-helix bundle (Figure 2), but it requires injection. Perhaps the most promising entry inhibitor approved for clinical use is Maraviroc, which binds to the transmembrane domains of CCR5 and prevents it from acting as an HIV co-receptor.
In theory, drugs such as Maraviroc that are targeted to cellular receptors should not evoke genetic resistance in the virus. However, HIV is artful when under strong selective pressure, and resistance does occur . Mutations in the V3 loop domain of gp120 allow Maraviroc-resistant viruses to interact more strongly with the amino-terminal extracellular domain of CCR5 and become less dependent on the second extracellular loop, which is closer to the drug’s binding site within the transmembrane regions .
Our knowledge of HIV receptors and how the envelope glycoproteins interact with them is also relevant for vaccine development . Immunogens that elicit antibodies that block receptor interaction should be protective. Rare monoclonal antibodies derived from naturally infected humans and from experimentally immunized animals recognize the CD4-binding site on gp120. As all the diverse HIV strains utilize CD4, some of these antibodies have breadth as well as potency in neutralizing almost all virus strains. However, designing a vaccine to elicit specifically such antibodies, which occur only rarely and late in natural infections, has proved challenging [34, 35].
Conclusions and prospects
This brief survey has touched upon the very first steps of HIV infection to illustrate how the pathogenesis, development of some types of anti-retroviral drugs and approaches to vaccine discovery have been aided by knowledge of receptors and entry processes. Once HIV gets into the cell, and begins to uncoat and to undergo reverse transcription, interactions with intracellular proteins, including restriction factors, kick in , which offer further opportunities for control of HIV infection. As with many viruses, the interaction of HIV with host cell components also illuminates fundamental aspects of cell and molecular biology since viruses are spanners in the works that tell us about the working of the cell.
Looking ahead, HIV entry processes may offer means of prevention of HIV infection in addition to vaccines. For example, blocking receptor interaction could be exploited for the development of novel types of vaginal microbicide based on mini-CD4 molecules and on broadly neutralizing mini-antibodies, which also have potential for blocking mucosal infection  of women. The advantage of such anti-HIV neutralizing agents in preventing infection would be to reduce the rapid spread of resistance that will inevitably follow the prophylactic use of the same drugs as are used for treating existing infection.
Yet there remains a huge gap between inventing clever means of blocking HIV infection in the laboratory and translating them into successful public health measures. This is exemplified in HIV vaccine development where the latest clinical trial has just been halted  because more cases of HIV-1 infection occurred in the vaccinated arm than in the placebo arm. For an HIV-infected person, being a ‘long-term non-progressor’, with well maintained CD4 cell counts and low viral load, provides an excellent prospect for survival with a reasonable quality of life. But as a vaccine researcher myself, I feel that while we have made interesting scientific discoveries along the way, regrettably we are long-term non-progressors!
Barré-Sinoussi F, Chermann JC, Rey F, Nugeyre MT, Chamaret S, Gruest J, Dauguet C, Axler-Blin C, Vézinet-Brun F, Rouzioux C, Rozenbaum W, Montagnier L: Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983, 220: 868-871. 10.1126/science.6189183.
CDC: Kaposi’s sarcoma and Pneumocystis pneumonia among homosexual men - New York City and California. MMWR. 1981, 30: 305-308.
Gottlieb MS, Schroff R, Schanker HM, Weisman JD, Fan PT, Wolf RA, Saxon A: Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency. N Engl J Med. 1981, 305: 1425-1431. 10.1056/NEJM198112103052401.
Ellrodt A, Barré-Sinoussi F, Le Bras P, Nugeyre MT, Palazzo L, Rey F, Brun-Vezinet F, Rouzioux C, Segond P, Caquet R, Montagnier L, Chermann JC: Isolation of human T-lymphotropic retrovirus (LAV) from Zairian married couple, one with AIDS, one with prodromes. Lancet. 1984, 1: 1383-1385.
Gallo RC, Salahuddin SZ, Popovic M, Shearer GM, Kaplan M, Haynes BF, Palker TJ, Redfield R, Oleske J, Safai B, White G, Foster P, Markham PD: Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984, 224: 500-503. 10.1126/science.6200936.
Levy JA, Hoffman AD, Kramer SM, Landis JA, Shimabukuro JM, Oshiro LS: Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science. 1984, 225: 840-842. 10.1126/science.6206563.
Weiss RA, Clapham PR, Cheingsong-Popov R, Dalgleish AG, Carne CA, Weller IV, Tedder RS: Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients. Nature. 1985, 316: 69-72. 10.1038/316069a0.
Margolick JB, Volkman DJ, Lane HC, Fauci AS: Clonal analysis of T lymphocytes in the acquired immunodeficiency syndrome. Evidence for an abnormality affecting individual helper and suppressor T cells. J Clin Invest. 1985, 76: 709-715. 10.1172/JCI112025.
Ndung’u T, Weiss RA: On HIV diversity. AIDS. 2012, 26: 1255-1260. 10.1097/QAD.0b013e32835461b5.
Doores KJ, Bonomelli C, Harvey DJ, Vasiljevic S, Dwek RA, Butron DR, Crispin M, Scanlan CN: Envelope glycans of immunodeficiency viruses are almost entirely oligomannose antigens. Proc Natl Acad Sci U S A. 2010, 107: 13800-13805. 10.1073/pnas.1006498107.
McCoy LE, Weiss RA: Neutralizing antibodies to HIV-1 induced by immunization. J Exp Med. 2013, 210: 209-223. 10.1084/jem.20121827.
Doms RW: Beyond receptor expression: the influence of receptor conformation, density and affinity in HIV-1 infection. Virology. 2000, 276: 229-237. 10.1006/viro.2000.0612.
Klatzmann D, Barré-Sinoussi F, Nugeyre MT, Dauguet C, Vilmer E, Griscelli C, Brun-Veziret F, Rouzioux C, Gluckman JC, Chermann JC, Montagnier L: Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes. Science. 1984, 225: 59-63. 10.1126/science.6328660.
Klatzmann D, Champagne E, Chamaret S, Gruest J, Guetard D, Hercend T, Gluckman JC, Montagnier L: T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV. Nature. 1984, 312: 767-768. 10.1038/312767a0.
Dalgleish AG, Beverley PC, Clapham PR, Crawford DH, Greaves MF, Weiss RA: The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature. 1984, 312: 763-767. 10.1038/312763a0.
Kwong PD, Wyatt R, Robinson J, Sweet RW, Sodroski J, Hendrickson WA: Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody. Nature. 1998, 393: 648-659. 10.1038/31405.
Rossmann MG: The canyon hypothesis: hiding the host cell receptor attachment site on a viral surface from immune surveillance. J Biol Chem. 1989, 264: 14587-14590.
Mendelsohn CL, Wimmer E, Racaniello VR: Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell. 1989, 56: 855-865. 10.1016/0092-8674(89)90690-9.
Maddon PJ, Dalgleish AG, McDougal JS, Clapham PR, Weiss RA, Axel R: The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell. 1986, 47: 333-348. 10.1016/0092-8674(86)90590-8.
Feng Y, Broder CC, Kennedy PE, Berger EA: HIV-1 entry cofactor: Functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996, 272: 872-877. 10.1126/science.272.5263.872.
Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P: Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells. Science. 1995, 270: 1811-1815. 10.1126/science.270.5243.1811.
Forsman A, Weiss RA: Why is HIV a pathogen?. Trends Microbiol. 2008, 16: 555-560. 10.1016/j.tim.2008.09.004.
Liu R, Paxton WA, Choe S, Ceradini D, Martin SR, Horuk R, MacDonald ME, Stuhlmann H, Koup RA, Landau NR: Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection. Cell. 1996, 86: 367-377. 10.1016/S0092-8674(00)80110-5.
Allers K, Hütter G, Hofmann J, Loddenkemper C, Rieger K, Thiel E, Schneider T: Evidence for the cure of HIV infection by CCR5Δ32/Δ32 stem cell transplantation. Blood. 2010, 117: 2791-2799.
Kulkarni H, Marconi VC, Agan BK, McArthur C, Crawford G, Clark RA, Dolan MJ, Ahuja SK: Role of CCL3L1-CCR5 genotypes in the epidemic spread of HIV-1 and evaluation of vaccine efficacy. PLoS One. 2008, 3: e3671-10.1371/journal.pone.0003671.
Geijtenbeek TB, Kwon DS, Torensma R, van Vliet SJ, van Duijnhoven GC, Middel J, Cornelissen IL, Nottet HS, KewalRamani VN, Littman DR, Figdor CG, van Kooyk Y: DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans- infection of T cells. Cell. 2000, 100: 587-597. 10.1016/S0092-8674(00)80694-7.
Wilen CB, Tilton JC, Doms RW: Molecular mechanisms of HIV entry. Adv Exp Med Biol. 2012, 726: 223-242. 10.1007/978-1-4614-0980-9_10.
Miyauchi K, Kim Y, Latinovic O, Morozov V, Melikyan GB: HIV enters cells via endocytosis and dynamin-dependent fusion with endosomes. Cell. 2009, 137: 433-444. 10.1016/j.cell.2009.02.046.
García-Expósito L, Ziglio S, Barroso-González J, de Armas-Rillo L, Valera MS, Zipeto D, Machado JD, Valenzuela-Fernández A: Gelsolin activity controls efficient early HIV-1 infection. Retrovirology. 2013, 10: 39-10.1186/1742-4690-10-39.
Jolly C, Sattentau QJ: Retroviral spread by induction of virological synapses. Traffic. 2004, 5: 643-650. 10.1111/j.1600-0854.2004.00209.x.
Murooka TT, Deruaz M, Marangoni F, Vrbanac VD, Seung E, von Andrian UH, Tager AM, Luster AD, Mempel TR: HIV-infected T cells are migratory vehicles for viral dissemination. Nature. 2012, 490: 283-287. 10.1038/nature11398.
De Feo CJ, Weiss CD: Escape from human immunodeficiency virus type 1 (HIV-1) entry inhibitors. Viruses. 2012, 4: 3859-3911. 10.3390/v4123859.
Berro R, Klasse PJ, Jakobsen MR, Gorry PR, Moore JP, Sanders RW: V3 determinants of HIV-1 escape from the CCR5 inhibitors Maraviroc and Vicriviroc. Virology. 2012, 427: 158-165. 10.1016/j.virol.2012.02.006.
McMichael A: In pursuit of an HIV vaccine: an interview with Andrew McMichael. BMC Biol. in press
Schiffner T, Sattentau QJ, Dorrell L: Development of prophylactic vaccines against HIV-1. Retrovirology. in press
Harris RS, Hultquist JF, Evans DT: The restriction factors of human immunodeficiency virus. J Biol Chem. 2012, 287: 40875-40883. 10.1074/jbc.R112.416925.
HIV vaccine trials network. [http://www.hvtn.org/505-announcement-25April2013.html]
My research has been supported by the UK Medical Research Council and the Bill & Melinda Gates Foundation Collaboration on AIDS Vaccine Discovery.
This article is part of the cross journal collection HIV 30 years on. Other articles in this series can be found at .
About this article
Cite this article
Weiss, R.A. Thirty years on: HIV receptor gymnastics and the prevention of infection. BMC Biol 11, 57 (2013). https://doi.org/10.1186/1741-7007-11-57
- cell receptors