Figure 4

Association of mRNAs with eIF3 complexes. (A) Messenger RNAs associated with eIF3e and eIF3m complexes were identified by microarray hybridization as described in the Methods section. The graph indicates the numbers of mRNAs enriched more than three-fold over mock in both complexes. (B) Classification into functional groups of the 106 mRNAs most highly and uniquely enriched in complexes with eIF3e or eIF3m. (C) The relative expression ranks of the 106 most highly and uniquely enriched mRNAs in the eIF3e and eIF3m complexeswere determined using the relative hybridization signals obtained with total S. pombe cDNA ([see additional file 1]). The expression ranks were blotted against the factor of enrichment of each mRNA in the eIF3 complexes. No correlation between expression rank and enrichment is apparent, indicating that highly expressed mRNAs were not unspecifically enriched in eIF3 complexes. The 5407 individual features on the microarray slides represent the 4988 ORFs and transcripts predicted in the Sanger Centre S. pombe genome database and various controls. To simplify the analysis, expression ranks were also assigned to these control spots, thus resulting in expression ranks for some ORFs higher than the theoretically possible number of 4988.