Alignment of sequences upstream the IL4I1 and variant IL4I1_2 open reading frames. Alignments of IL4I1 (upper panel) and variant IL4I1_2 (lower panel) sequences are shown. The first two exons of IL4I1 are shown, and splice sites are underlined and in bold. Ten nucleotides of the next intron are displayed downstream of the first coding exon, which all match the consensus splice donor sequence. Reading frames are in all caps, and the deduced peptide sequences are given below. The human and chimpanzee sequences of IL4I1_2 have an upstream and in-frame start codon (bold), which is not conserved in mouse, rat or dog, and which does not match the Kozak consensus rules. All IL4I1_2 sequences have a short upstream ORF (all caps) which is conserved in location and sequence. The mouse, rat and human sequences were derived from cDNA sequences, while the chimpanzee and dog sequences were deduced from an alignment of human cDNA with the respective genome sequences .