A comparison of Totivirus and endogenous Totivirus -like fungal gene products. Pichia stipitis (lane 1), Saccharomyces cerevisiae with exogenous Totivirus infection (lane 2) and Debaryomyces hansenii (lane 3) (A) polymerase chain reaction (PCR) of total DNA (1.4% agarose gel). (B) Assay for dsRNA (1.4% agarose gel with EtBr staining). (C) Assay for viral particles (photograph of CsCl density gradients). (D) Reverse transcriptase (RT)-PCR of total RNA (1.4% agarose gel). (E) PCR of total RNA extractions treated with DNAse. Here, RT-PCR of Cp is attempted from primers that flank the Cp-RdRp boundary. Reverse transcriptase is marked as either present (+RT) or absent (-RT).