- Research article
- Open Access
The evolution of novel fungal genes from non-retroviral RNA viruses
BMC Biology volume 7, Article number: 88 (2009)
Endogenous derivatives of non-retroviral RNA viruses are thought to be absent or rare in eukaryotic genomes because integration of RNA viruses in host genomes is impossible without reverse transcription. However, such derivatives have been proposed for animals, plants and fungi, often based on surrogate bioinformatic evidence. At present, there is little known of the evolution and function of integrated non-retroviral RNA virus genes. Here, we provide direct evidence of integration by sequencing across host-virus gene boundaries and carry out phylogenetic analyses of fungal hosts and totivirids (dsRNA viruses of fungi and protozoans). Further, we examine functionality by tests of neutral evolution, comparison of residues that are necessary for viral capsid functioning and assays for transcripts, dsRNA and viral particles.
Sequencing evidence from gene boundaries was consistent with integration. We detected previously unknown integrated Totivirus-like sequences in three fungi (Candida parapsilosis, Penicillium marneffei and Uromyces appendiculatus). The phylogenetic evidence strongly indicated that the direction of transfer was from Totivirus to fungus. However, there was evidence of transfer of Totivirus-like sequences among fungi. Tests of selection indicated that integrated genes are maintained by purifying selection. Transcripts were apparent for some gene copies, but, in most cases, the endogenous sequences lacked the residues necessary for normal viral functioning.
Our findings reveal that horizontal gene transfer can result in novel gene formation in eukaryotes despite miniaturized genomic targets and a need for co-option of reverse transcriptase.
In eukaryotes, novel genes can be formed by alternative splicing, exon shuffling, horizontal gene transfer and inserted retroelements [1, 2]. Indeed, many eukaryotic genomes are bloated with the raw materials (introns and retroelements) for these processes [3–5]. In most budding yeasts, however, the source of novel gene formation is obscure as there is a dearth of spliceosomal introns (< 5% of genes and < 0.5% of the genome) and retroelement products (< 3% of genome size) [6–8]. Although duplication of existing genes is common in budding yeasts, de novo gene formation and the horizontal gene transfer (HGT) appear stifled by the architecture of miniaturized genomes [7, 9]. It is surprising, then, that NCBI Genbank annotations (EU380679 from this study, CAR65487, ABN68085, and ABN68086) and the BLAST-based study of Frank and Wolfe  reveal significant matches of yeast genes to the non-retroviral dsRNA viruses Saccharomyces cerevisiae L-A (L1) virus (Totiviridae). Debaryomyces hansenii has two capsid (Cp)-like genes, while Pichia stipitis has at least four Cp-like genes; each fungus has a single RNA dependent RNA polymerase (RdRp)-like gene . Endogeny is common for viruses that either encode their own reverse transcriptase (retroviruses and pararetroviruses) or are already DNA-based [11–13] but fragments of integrated non-retroviral viral RNA have rarely been proposed [14–16]. As the integration of non-retroviral RNA viruses into DNA-based eukaryotic genomes requires the co-option of reverse transcriptase , Holmes  called this type of transfer 'one of the most remarkable observations in viral evolution of recent years'.
Little is known of the biology of non-retroviral integrated RNA viruses (NIRVs). We are unaware, for example, of evolutionary or functional comparisons among NIRVs. Even the initial bioinformatic evidence of NIRVs is often weak as genome assemblies can be contaminated or incorrectly annotated and surrogate (non-phylogenetic) analyses are susceptible to false positives [18–20]. The proposed transfer of bacterial genes into the human genome, for example, disappeared with a detailed phylogenetic analysis . The initial claims of NIRVs in grape (Vitus) genomes also failed the direct tests of integration . Direct evidence of NIRVs is provided by the successful polymerase chain reaction (PCR) amplification or sequencing across host and integrated virus gene boundaries . Where HGT is strongly supported, BLAST-based analyses of genes with open reading frames cannot adequately discern the direction of the transfer (from host to virus or from virus to host). The dsRNA elements that code for killer toxins in some fungi appear to have a cellular origin based on structural similarities to cellular genes and, in some cases, the preservation of vestigial polyA sequences at internal positions of the viral plus strands . However, another BLAST-based study concluded that significant sequence matches with cellular genes and the presence of cellular pseudogenes indicated the transfer from killer dsRNA element to cellular genome . The resolution of the question of directionality and the determination of HGT requires phylogenetic evidence with strong support and adequate sampling of interacting organisms .
Nor can functional maintenance of suspected HGT genes be inferred solely from a putative open reading frame - recent pseudogenes can produce a similar pattern . Evolutionary analysis of selection and analysis of transcription products can provide stronger evidence of functional maintenance. Though negative evidence, a lack of viral products (dsRNA or viral particles), might hint at a novel function in a NIRV. Further evidence can be provided by the comparative analysis of functional landmarks. For example, the capsid gene of the Totivirus (L1-LA) functions to remove m7GMP caps from fungal host mRNAs [25, 26]. Site directed mutational experiments have revealed eight residues that are essential for this decapping function [25, 26]. Alteration of these residues in an integrated viral Cp gene would provide evidence for novel function. In the present study we carry out phylogenetic, evolutionary and functional analyses to test the hypothesis of NIRVs, the direction of HGT and the hypothesis that HGT of non-retroviral RNA viruses results in novel functioning genes in the fungal-Totiviridae system.
Totivirids are simple dsRNA viruses defined by the presence of the RdRp and the single capsid polypeptide (Cp) on a single dsRNA . The isometric virions are about 40 nm in diameter. Inheritance of these intracellular RNA viruses is normally vertical (no cellular genomic copies of the dsRNA genomes are present) but horizontal transfer can occur across fungal hyphae . Confirmed intracellular totivirid infections are known only from fungi and pathogenic protozoans (Leishmania, Giardia and Trichonomas). The totivirids in smut and yeast have been assigned to the genus Totivirus, whereas totivirids infecting filamentous fungi have been assigned to the genus Victorivirus . Here, we provide independent direct evidence that Totivirus-like genomes are integrated into the cellular genomes of yeast that lack Totivirus infections and phylogenetic evidence that genes of Totivirus-like viruses have been transferred to the genomes of four yeast species. Moreover, the endogenous Totivirus-like genes appear to be functionally maintained with non-viral functions and may have been further transferred between deeper yeast lineages.
Results and discussion
BLAST analysis using the RdRp and Cp protein sequences of the S. cerevisiae virus La (L-Bc) as a query, detected Totivirus-like genes in five fungal species. We found RdRp-like sequences in P. stipitis, Penicillium marneffei, D. hansenii and Uromyces appendiculatus, and Cp-like sequences in P. stipitis, P. marneffei, D. hansenii, and Candida parapsilosis. The U. appendiculatus match is from an Expressed Sequence Tags (EST) database. The D. hansenii virus-like genome has two overlapping reading frames, as in the exogenous viral architecture of the S. cerevisiae L1 virus (ScVL1 or ScVL-A; Figure 1; . The overlap is 138 bases and the RdRp-like gene can be translated as a minus one (-1) programmed ribosomal frameshift with the slippery site, GGGUUUA [30, 31], as in ScVL1. The P. stipitis virus-like genes encode a Cp-RdRp fusion protein, and has a similar architecture to that found in the Ustilago maydis P1H1 virus UmV . The P. marneffei genome also appears to contain a Totivirus-like genome (Figure 2). Here the overlap between the CP-like gene and the RdRp-like gene is 141 bases, but the slippery site that would allow a minus one translation has been altered by a point mutation (GGAUUUA), suggesting a non-viral function. No nonviral-like sequence is available from existing contigs of P. marneffei, so further sequencing would be needed in order to confirm the fungal flanking regions. For C. parapsilosis, it is clear that only a Cp-like sequence is present on the large contig where we detected a Totivirus-like gene (Figure 2).
We tested for the presence of DNA copies of dsRNA Totivirus-like genes by PCR amplification of fungal DNA extractions with specific primers (Figure 1). The first assay, targeting the RdRp-Cp boundary, revealed an expected PCR product size from both P. stipitis (488 bp) and D. hansenii (1172 bp), while S. cerevisiae, which possesses only viral dsRNA targets, lacked a detectable PCR product. This result suggests that DNA-based genes matching the viral sequences are present in D. hansenii and P. stipitis cells. We then tested for fungal integration of these genes by PCR amplification and DNA sequencing across the proposed fungal-viral genome boundaries. We sequenced the RdRp-fungus and upstream Cp-fungus boundaries for each fungal species (Figure 1). The experimental sequences have 100% matches with the proposed yeast genome assemblies containing both the expected yeast and the viral-like sequences. A direct test of the integration in the remaining three fungi where we detected Totivirus-like sequences is pending.
The fungal RdRp-like sequences form a close, and strongly supported, derived group within the viral RdRp gene tree (Figure 3). Thus, the data satisfy the phylogenetic criterion of horizontal transfer - a strongly supported phylogenetic incongruence between interacting organisms . As many fungal genomes are now known, the close association of the viral-like RdRp gene in hemiascomycetous yeast (P. stipitis and D. hansenii) with the RdRp gene of exogenous Totivirus of other hemiascomycetes, such as S. cerevisiae (Figures 3-4), is unlikely to be a sampling artifact or a differential gene loss of Totivirus-like genes in fungi. Instead, the placement of virus-like fungal sequences at the tips of the totivirid tree indicates that the endogenous forms in hemiascomycetes evolved from Totivirus and not from fungal genomes. The Totivirus-like genes of P. marneffei, a Euascomycote (Additional File 1), are also nested within the hemiascomycete clade and are most closely related to D. hansenii. The presence of closely related Totivirus RdRp-like sequences in fungi from two divergent clades suggests the occurrence of multiple integration events or horizontal transfers among yeasts. The position of the fungal sequences within the hemiascomycete virus clade suggests that integration occurred first in hemiascomycetes and was transferred to P. marneffei. Further evidence of homology of NIRVs might be provided by similarity of chromosomal regions flanking the insertion site. However, even the most closely related fungi in our study have undergone extensive chromosomal rearrangements. Jeffries et al.  found no orthologous chromosomal segments between the center of CHR 7 in P. stipitis and chromosome B of D. hansenii, the locations of the NIRVs. The fungal gene tree (Additional File 1) reveals that the fungal species with endogenous Totivirus-like genes, save P. marneffei, belong to the Clavispora clade , which has Candida lusitaniae as the representative species. The Clavispora clade, which has sometimes been called the Candida clade  and is characterized by a shift in genetic code, is estimated to be at least 100 million years old .
The Cp gene tree also revealed a close relationship of the endogenous viruses to the Totivirus of Hemiascomycotes (Figure 4). BLAST matches are apparently limited to the genus Totivirus because the Cp gene has evolved more rapidly than the RdRp gene (Figures 3-4). The two copies of Cp-like genes within D. hansenii have a sister relationship (amino-acid p-distance = 0.060) and represent either recent paralogous duplication or concerted evolution. However, the four copies of Cp-like genes in P. stipitis are much more divergent with the average amino p-distance between Cp1 and the other Cp copies in Pichia at 0.755. The tandem positioning of two to four divergent Cp gene copies and the monophyly of the fungal viral-like genes is consistent with the hypothesis of endogenous tandem duplication after integration by a Totivirus-like dsRNA viral lineage. Interestingly, the ancestral viral genomic architecture appears intact in P. stipitis (similar to UmV, see above)D. hansenii (similar to ScVL1) and P. marneffei which further supports the viral genome transfer hypothesis and permits the diagnosis of the ancestral integrated gene in the Cp-like gene family (Figures 1-2). Alternative scenarios, where Totivirus genomes are repeatedly and faithfully duplicated in toto or are independently integrated at the identical regions in these fungal genomes, are unlikely.
Under the scenario of endogeny we expect patterns of DNA substitutions to differ between exogenous viral and integrated fungal gene copies. A disparity index revealed that fungal sequences do differ from viral sequences in patterns of DNA substitution more than is expected from evolutionary distance or from chance alone (Additional File 2). Apart from the Cp1 sequence in P. stipitis, there were no significant differences among disparity indices within the fungal sequences or within the viral sequences. Thus, our sequencing of gene boundaries and the substitution patterns are consistent with integrated fungal copies of dsRNA Totivirus-like genes.
Despite pronounced sequence divergence, each of the Totivirus-like fungal genes had an uninterrupted open reading frame (Figures 1-2). By searching the translated EST database for Pichia with the RdRp amino acid sequence (tBLASTn), we found that the RdRp mRNA product appears as a normal, polyadenylated RNAPII transcript in EST libraries (FE843929.1 and FE843928.1). Similarly, we found matching RNAPII transcripts of at least two of the Cp genes, Cp2 (FE851263.1 and FE851264.1) and Cp4 (FE849285.1 and FE849284.1) in P. stipitis. In order to test whether the endogenous genes are evolving as functional genes, we calculated pairwise and phylogenetic tests of neutral evolution (Table 1; Figure 5). Each of the comparisons for endogenous fungal genes revealed a significant departure from neutrality in the direction of purifying selection, consistent with purifying selection to maintain gene function (Table 1; Figure 5).
As we found evidence for viral genome architecture in our PCRs (Figures 1 and 6A), we examined if the endogenous genes functioned as in dsRNA viruses. We failed to detect dsRNA products (Figure 6B) or viral particles (empty or full) in P. stipitis and in D. hansenii (Figure 6C). This contrasts with the positive detection of dsRNA and viral particles in the Totivirus containing cells of S. cerevisiae. A reverse transcriptase PCR (RTPCR) experiment revealed no detectable transcripts of the complete endogenous Totivirus genomes in D. hansenii or in P. stipitis (targets include the Cp-RdRp boundary, Figure 6D). Notably, RTPCR with oligonucleotide primers targeting only the RdRp region did contain transcripts (Figure 6E). RNA from the Totivirus-containing S. crevisiae does give an RTPCR product from the Cp-RdRp region. These results indicate that endogenous gene transcription proceeds differently than in Totivirus, as the integrated RdRp sequences in D. hansenii and in P. stipitis initiate within the Cp sequence, creating a subgenomic mRNA. Further, six to seven of eight biochemically-conserved residues that are important to the decapping function for Totivirus Cp genes [25, 26] are altered in three of the endogenous Cp-like genes of P. stipitis (Figure 7).
Taken together, the functional and comparative evidence suggests that the endogenous viral proteins have been co-opted for cellular functions. The abandonment of viral expression is also indicated by the absence of recognizable RNA pseudoknot structure, following the slippery site in the Totivirus-like genomes of Pichia and P. marneffei, and by the absence of recognizable proteinase cleavage sites or proteinase active site motifs in the Cp-RdRp overlap region of D. hansenii. Co-option of integrated DNA viruses and retroviruses by multicellular eukaryotes is well known but rare . An integration mechanism that co-opts endogenous RNA integration machinery is plausible as yeast genomes contain retroelements [9, 35]. However, the signature of retroelements is not obvious in the flanking regions of the present integrations, perhaps as a result of evolutionary divergence.
The evolutionary distribution and stabilization of NIRVs is poorly understood. Opportunities for NIRV formation should be greater in host taxa that have evolved a longstanding (presumably hypovirulent) non-retroviral virus infection, as in the fungi-Totivirus or the dipteran-flavivirus associations. However, the Clavispora clade, which we identify as the source of the yeast NIRVs, appears to lack such infections with Totivirus. Given the widespread taxonomic distribution of Totivirus-like infections in fungi and their absence in Clavispora , it is tempting to invoke the shift in genetic code as a contributor to the loss of infectionby Totivirus. Holmes  proposed that shifts in genetic codes are evolutionary responses by the host to RNA-based viral infections. NIRVs could have initially played a beneficial role to the host by imparting resistance to exogenous viruses. Indeed, there is experimental evidence in the yeast-Totivirus system that overproduction of the capsid protein  or production of fragments of the capsid protein will interfere with packaging of the virus and result in its loss. Such interference is also well-documented with plant viruses . The apparent genomic prevalence of capsid-like NIRVs compared to RdRp-like NIRVs (Figures 1 and 2) is consistent with this interference hypothesis. In either case, the NIRVs that we find in the Clavispora clade today could represent the vestiges of a co-evolutionary victory by the host.
We conclude that novel eukaryotic gene families have originated from non-retroviral RNA viruses. NIRVs and their transitional stages are archived in eukaryotic genomes and appear more important to yeast evolution than previously thought.
We obtained lyophilized powders of P. stipitis strain CBS6054  from T Jeffries, Debaryomyces hansenii strain CBS767  from Jean-Luc Souciet and Saccharomyces cerevisiae strain S7 from C McLaughlin , which has the S. cerevisiae virus L1 (LA) and the minor virus La (LB-C) but no satellite virus. Cells were streaked on YPD agar (yeast extract 1%, peptone 2%, and dextrose 2%) and single colonies were transferred to 150 ml of YPD broth.
Nucleic acid and viral particle extractions
RNA was extracted from cells with the Masterpure Yeast RNA extraction kit (Epicentre, WI, USA). DNA was extracted using a standard SDS/phenol/chloroform protocol. Viral dsRNA was isolated by CF11 chromatography from crude RNA preparations . Empty viral particles of density 1.33 g/cc were isolated as described , except that polyethylene glycol precipitation was replaced by high-speed pelleting of viral particles (100,000 × g for 1.5 h).
PCR, RTPCR and DNA sequencing
Fifty microlitre PCR reactions contained 5 μL of extracted DNA template, 10× PCR buffer [50 mM KCl, 1.5 mg MgCl2, 10 mM Tris-HCl pH 8.3, 0.01% (w/v) gelatin], 2 mM of each dNTP, 1 μM of each primer and 1 unit of Taq DNA polymerase. Primers are listed in Additional File 3. The PCR temperature profiles were: 30 cycles of 94°C for 30 s; 55°C for 30 s; 72°C for 2 min; and final extension at 72°C for 5 min. RT-PCR detection of viral transcripts and PCR of genomic DNA copies (Epicentre high fidelity RT-PCR kit) were performed as described by the manufacturer. Gels were 1.4% agarose in Tris-acetate-EDTA (40 mM Tris-acetate and 1 mM EDTA, pH 8.3) stained with ethidium bromide (1 mg/l). DNA was sequenced by Sanger methods at the University of Washington High Throughput Genomics Facility. Sequencher 4.8 was used to assemble and edit electrophoregrams. New sequences from this study have the following Genbank accession numbers: EU380679 and GQ291318-GQ291321.
We obtained the initial sequences of RdRp (Additional File 4) and capsid (Additional File 5) genes from totivirids by BLASTp of the nr peptide sequence database (National Center for Biotechnology Information, Bethesda, USA) with the protein sequences of S. cerevisiae virus La (L-BC) and a cut-off of E<0.01. Additional genomic copies of Totivirus-like genes were identified by significant tBLASTx hits (E<0.05) of relevant NCBI BLAST databases using the sequences of S. cerevisiae virus La (L-BC). For the capsid proteins the entire sequences were used and, for RdRp, the contiguous conserved region  was used. Sequences were aligned using Prank: probabilistic alignment kit with the Pranskter graphical interface (Goldman Group, European Bioinformatics Institute, Cambridge, United Kingdom) . We carried out maximum likelihood analyses with RAxML using the RTREV substitution matrix, estimated AA frequencies, a gamma parameter for among-site rate variation, and an invariable sites parameter . For bootstrapping, RAxML estimated the number of pseudoreplicates. For Bayesian analysis, we used Mr Bayes  with an amino acid substitution model prior of RTREV with a setting of rates = INVGAMMA. After 1 million Markov chain Monte Carlo generations and confirming convergence (average standard deviation of split frequencies < 0.01 and a plot of log likelihood scores with generation), we culled a burn-in set of 10,000 trees and calculated the posterior probabilities. Trees were midpoint rooted.
For the phylogeny of yeasts, we used the five genes (Additional File 6) with the greatest number of strong reliability values from the list of the best 10 performing genes for recovering fungal phylogeny (as determined by a genome-scale analysis  for topological correctness). For fungi, orthologous nuclear genes with strong support values for a given node (bootstrap > 90 and posterior probabilities > 0.95) rarely disagree [46, 47]. We chose species from FUNYBASE  that had all of the genes of interest, and added genomic sequences from an additional species of Schizosaccharomyces, and from three genomes where we detected totivirid-like sequences (Additional File 1; a fourth genome with totivirid sequences, D. hansenii was already part of FUNYBASE). Concatenated sequences were aligned in MAFFT  and then exposed to culling from GBlocks . We carried out maximum likelihood and Bayesian analyses as for the totivirid alignments above.
A test of the homogeneity of substitution patterns between viral and fungal sequences for the Cp-like genes was carried out in MEGA4 . We used the Disparity Index test  with P-values estimated from 1000 Monte Carlo based replicates (Additional File 2). The most divergent sequence (S. cerevisiae virus La (L-BC)) and the shortest sequence (C. parapsilosis) were culled for this analysis because we wanted to retain informative alignment positions when gapped sites are completely deleted. After positions with gaps and missing data were eliminated a dataset of 1446 positions was retained.
Tests of neutral evolution for the Cp-like gene copies in fungi were carried out in MEGA4. The test statistic is (dN - dS) where dS and dN are the numbers of synonymous and nonsynonymous substitutions per site, respectively. The variance of the difference was computed using the bootstrap method (500 replicates). Analyses were conducted using the Kumar method in MEGA4 . Ka/Ks ratios were calculated by the Ka/Ks calculator http://services.cbu.uib.no/tools/kaks and plotted on a tree using the methods of . GC content was estimated from the alignment. The ratio of nonsynonymous (Ka) to synonymous (Ks) nucleotide substitution rates is an indicator of selective pressures on genes. A ratio of less than one indicates selective pressure to conserve protein sequence. Note that values are averaged over sites.
expressed sequence tag
horizontal gene transfer
non-retroviral integrated RNA virus
polymerase chain reaction
RNA dependant RNA polymerase
reverse transcriptase PCR.
Long M, Betran E, Thornton K, Wang W: The origin of new genes: glimpses from the young and old. Nat Rev Genet. 2003, 4 (11): 865-875. 10.1038/nrg1204.
Doolittle WF: You are what you eat: a gene transfer ratchet could account for bacterial genes in eukaryotic nuclear genomes. Trends Genet. 1998, 14 (8): 307-311. 10.1016/S0168-9525(98)01494-2.
Roy SW, Gilbert W: The evolution of spliceosomal introns: patterns, puzzles and progress. Nat Rev Genet. 2006, 7 (3): 211-221.
Meyers BC, Tingey SV, Morgante M: Abundance, distribution, and transcriptional activity of repetitive elements in the maize genome. Genome Res. 2001, 11 (10): 1660-1676. 10.1101/gr.188201.
Kazazian HH: Mobile elements: drivers of genome evolution. Science. 2004, 303 (5664): 1626-1632. 10.1126/science.1089670.
Dujon B, Sherman D, Fischer G, Durrens P, Casaregola S, Lafontaine I, De Montigny J, Marck C, Neuvéglise C, Talla E, Goffard N, Frangeul L, Aigle M, Anthouard V, Babour A, Barbe V, Barnay S, Blanchin S, Beckerich JM, Beyne E, Bleykasten C, Boisramé A, Boyer J, Cattolico L, Confanioleri F, De Daruvar A, Despons L, Fabre E, Fairhead C, Ferry-Dumazet H, et al: Genome evolution in yeasts. Nature. 2004, 430 (6995): 35-44. 10.1038/nature02579.
Dujon B: Yeasts illustrate the molecular mechanisms of eukaryotic genome evolution. Trends Genetics. 2006, 22 (7): 375-387. 10.1016/j.tig.2006.05.007.
Jeffries TW, Grigoriev IV, Grimwood J, Laplaza JM, Aerts A, Salamov A, Schmutz J, Lindquist E, Dehal P, Shapiro H, Jin YS, Passoth V, Richardson PM: Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis. Nature Biotechnol. 2007, 25 (3): 319-326. 10.1038/nbt1290.
Butler G, Rasmussen MD, Lin MF, Santos MA, Sakthikumar S, Munro CA, Rheinbay E, Grabherr M, Forche A, Reedy JL, et al: Evolution of pathogenicity and sexual reproduction in eight Candida genomes. Nature. 2009, 459 (7247): 657-662. 10.1038/nature08064.
Frank AC, Wolfe KH: Evolutionary capture of viral and plasmid DNA by yeast nuclear chromosomes. Eukaryot Cell. 2009, 8 (10): 1521-1531. 10.1128/EC.00110-09.
Flegel TW: Hypothesis for heritable, anti-viral immunity in crustaceans and insects. Biol Direct. 2009, 4: 32-10.1186/1745-6150-4-32.
Holmes EC: The Evolution and Emergence of RNA Viruses. 2009, New York: Oxford University Press
Staginnus C, Iskra-Caruana ML, Lockhart B, Hohn T, Richert-Poggeler KR: Suggestions for a nomenclature of endogenous pararetroviral sequences in plants. Arch Virol. 2009, 154 (7): 1189-1193. 10.1007/s00705-009-0412-y.
Crochu S, Cook S, Attoui H, Charrel RN, De Chesse R, Belhouchet M, Lemasson JJ, de Micco P, de Lamballerie X: Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. mosquitoes. J Gen Virol. 2004, 85 (Pt 7): 1971-1980. 10.1099/vir.0.79850-0.
Maori E, Tanne E, Sela I: Reciprocal sequence exchange between non-retro viruses and hosts leading to the appearance of new host phenotypes. Virology. 2007, 362 (2): 342-349. 10.1016/j.virol.2006.11.038.
Tanne E, Sela I: Occurrence of a DNA sequence of a non-retro RNA virus in a host plant genome and its expression: evidence for recombination between viral and host RNAs. Virology. 2005, 332 (2): 614-622. 10.1016/j.virol.2004.11.007.
Geuking MB, Weber J, Dewannieux M, Gorelik E, Heidmann T, Hengartner H, Zinkernagel RM, Hangartner L: Recombination of retrotransposon and exogenous RNA virus results in nonretroviral cDNA integration. Science. 2009, 323 (5912): 393-396. 10.1126/science.1167375.
Keeling PJ, Palmer JD: Horizontal gene transfer in eukaryotic evolution. Nat Rev Genet. 2008, 9 (8): 605-618. 10.1038/nrg2386.
Ragan MA, Harlow TJ, Beiko RG: Do different surrogate methods detect lateral genetic transfer events of different relative ages?. Trends Microbiol. 2006, 14 (1): 4-8. 10.1016/j.tim.2005.11.004.
Ragan MA: On surrogate methods for detecting lateral gene transfer. FEMS Microbiol Lett. 2001, 201 (2): 187-191. 10.1111/j.1574-6968.2001.tb10755.x.
Stanhope MJ, Lupas A, Italia MJ, Koretke KK, Volker C, Brown JR: Phylogenetic analyses do not support horizontal gene transfers from bacteria to vertebrates. Nature. 2001, 411 (6840): 940-944. 10.1038/35082058.
Bertsch C, Beuve M, Dolja VV, Wirth M, Pelsy F, Herrbach E, Lemaire O: Retention of the virus-derived sequences in the nuclear genome of grapevine as a potential pathway to virus resistance. Biol Direct. 2009, 4: 21-10.1186/1745-6150-4-21.
Bruenn J: The Ustilago maydis viruses. Encyclopedia of Virology. Edited by: Mahy BWJ, van Regenmortel MHV. 2008, Amsterdam: Elsevier, 5: 214-219. full_text. 3
Nikoh N, Nakabachi A: Aphids acquired symbiotic genes via lateral gene transfer. BMC Biol. 2009, 7: 12-10.1186/1741-7007-7-12.
Naitow H, Tang J, Canady M, Wickner RB, Johnson JE: L-A virus at 3.4 A resolution reveals particle architecture and mRNA decapping mechanism. Nat Struct Biol. 2002, 9 (10): 725-728. 10.1038/nsb844.
Tang J, Naitow H, Gardner NA, Kolesar A, Tang L, Wickner RB, Johnson JE: The structural basis of recognition and removal of cellular mRNA 7-methyl G 'caps' by a viral capsid protein: a unique viral response to host defense. J Mol Recognit. 2005, 18 (2): 158-168. 10.1002/jmr.724.
Ghabrial SA: Origin, adaptation and evolutionary pathways of fungal viruses. Virus Genes. 1998, 16 (1): 119-131. 10.1023/A:1007966229595.
Hastie ND, Brennan V, Bruenn J: No homology between double-stranded RNA and nuclear DNA of yeast. J Virol. 1978, 28: 1002-1005.
Ghabrial S: Totiviruses. Encyclopedia of Virology. Edited by: BWJ Mahy, van Regenmortel MHV. 2008, Academic Press, 5: 163-174. full_text. 3
Diamond ME, Dowhanick JJ, Nemeroff ME, Pietras DF, Tu C-L, Bruenn JA: Overlapping genes in a yeast dsRNA virus. J Virol. 1989, 63: 3983-3990.
Tzeng T-H, Tu C-L, Bruenn JA: Ribosomal frameshifting requires a pseudoknot in the yeast double-stranded RNA virus. J Virol. 1992, 66 (2): 999-1006.
Kang J, Wu J, Bruenn JA, Park C: The H1 double-stranded RNA genome of Ustilago maydis virus-H1 encodes a polyprotein that contains structural motifs for capsid polypeptide, papain-like protease, and RNA-dependent RNA polymerase. Virus Res. 2001, 76 (2): 183-189. 10.1016/S0168-1702(01)00250-7.
Taylor JW: Evolution of human-pathogenic fungi: phylogenies and species. Molecular Principles of Fungal Pathogenesis. Edited by: Heitman J, Filler SG, Edwards JE Jr, Mitchell AP. 2006, Washington D.C.: ASM press, 113-132.
Jern P, Coffin JM: Effects of retroviruses on host genome function. Annu Rev Genet. 2008, 42: 709-732. 10.1146/annurev.genet.42.110807.091501.
Lesage P, Todeschini AL: Happy together: the life and times of Ty retrotransposons and their hosts. Cytogenet Genome Res. 2005, 110 (1-4): 70-90. 10.1159/000084940.
Valle RP, Wickner RB: Elimination of L-A double-stranded RNA virus of Saccharomyces cerevisiae by expression of gag and gag-pol from L-A cDNA clone. J Virol. 1993, 67 (5): 2764-2771.
Yao W, Bruenn JA: Interference with replication of two double-stranded RNA viruses by production of N-terminal fragments of capsid polypeptides. Virology. 1995, 214: 215-221. 10.1006/viro.1995.9938.
Reimann-Philipp U: Mechanisms of resistance: expression of coat protein. Methods Mol Biol. 1998, 81: 521-532.
Holm CA, Oliver SG, Newman AM, Holland LE, McLaughlin CS, Wagner EK, Warner RC: The molecular weight of yeast P1 double-stranded RNA. J Biol Chem. 1978, 253: 8332-8336.
Franklin RM: Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci USA. 1966, 55: 1504-1511. 10.1073/pnas.55.6.1504.
Naitow H, Canady MA, Lin T, Wickner RB, Johnson JE: Purification, crystallization, and preliminary X-ray analysis of L-A: a dsRNA yeast virus. J Struct Biol. 2001, 135 (1): 1-7. 10.1006/jsbi.2001.4371.
Loytynoja A, Goldman N: Phylogeny-aware gap placement prevents errors in sequence alignment and evolutionary analysis. Science. 2008, 320 (5883): 1632-1635. 10.1126/science.1158395.
Stamatakis A, Hoover P, Rougemont J: A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol. 2008, 57 (5): 758-771. 10.1080/10635150802429642.
Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19 (12): 1572-1574. 10.1093/bioinformatics/btg180.
Aguileta G, Marthey S, Chiapello H, Lebrun MH, Rodolphe F, Fournier E, Gendrault-Jacquemard A, Giraud T: Assessing the performance of single-copy genes for recovering robust phylogenies. Syst Biol. 2008, 57 (4): 613-627. 10.1080/10635150802306527.
Taylor DJ, Piel WH: An assessment of accuracy, error, and conflict with support values from genome-scale phylogenetic data. Mol Biol Evol. 2004, 21 (8): 1534-1537. 10.1093/molbev/msh156.
Rasmussen MD, Kellis M: Accurate gene-tree reconstruction by learning gene- and species-specific substitution rates across multiple complete genomes. Genome Res. 2007, 17 (12): 1932-1942. 10.1101/gr.7105007.
Marthey S, Aguileta G, Rodolphe F, Gendrault A, Giraud T, Fournier E, Lopez-Villavicencio M, Gautier A, Lebrun MH, Chiapello H: FUNYBASE: a FUNgal phYlogenomic dataBASE. Bmc Bioinformatics. 2008, 9: 456-10.1186/1471-2105-9-456.
Katoh K, Asimenos G, Toh H: Multiple alignment of DNA sequences with MAFFT. Methods Mol Biol. 2009, 537: 39-64. full_text.
Talavera G, Castresana J: Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol. 2007, 56 (4): 564-577. 10.1080/10635150701472164.
Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24 (8): 1596-1599. 10.1093/molbev/msm092.
Kumar S, Gadagkar SR: Disparity index: a simple statistic to measure and test the homogeneity of substitution patterns between molecular sequences. Genetics. 2001, 158 (3): 1321-1327.
Liberles DA: Evaluation of methods for determination of a reconstructed history of gene sequence evolution. Mol Biol Evol. 2001, 18 (11): 2040-2047.
We thank the University at Buffalo for support, Thomas W Jeffries for P. stipitis strain CBS 6054, Jean-Luc Souciet for D. hansenii strain CBS767, and C McLaughlin for S. cerevisiae strain S7. Mahbuba Meem and Isabelle Kim aided in nucleic acid extractions and in PCR protocols.
DJT and JB conceived the study and co-wrote the paper. JB carried out dsRNA and viral particle isolations, designed primers and identified residues essential for capsid functioning. DJT carried out DNA sequence assembly, PCR, phylogenetics and evolutionary analyses.
Electronic supplementary material
Additional file 1: Midpoint rooted maximum likelihood phylogram of yeast-like fungi based on a concatenation of the five single copy protein-coding genes identified as the most phylogenetically reliable in fungal genomes. (PDF 268 KB)
Additional file 2: Test of the homogeneity of substitution patterns between fungal and viral copies of capsid-like protein nucleotide sequences. (DOC 42 KB)
Additional file 3: Primers used for polymerase chain reaction (PCR) and reverse transcriptase-PCR of Totivirus-like regions of yeast genomes and exogenous Totivirus. (DOC 42 KB)
Additional file 4: Viral and fungal sequences and accession numbers used for phylogenetic analysis of the RdRp-like regions of totivirids and Totivirus-like sequences in fungi. (DOC 50 KB)
Additional file 5: Viral and fungal accession numbers used for phylogenetic analysis of the Cp-like regions of totivirids and Totivirus-like sequences in fungi. (DOC 34 KB)
Additional file 6: Fungal genes and accession numbers used for phylogenetic analysis of the budding yeasts. (DOC 72 KB)
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Taylor, D.J., Bruenn, J. The evolution of novel fungal genes from non-retroviral RNA viruses . BMC Biol 7, 88 (2009). https://doi.org/10.1186/1741-7007-7-88
- Horizontal Gene Transfer
- Candida Parapsilosis
- Fungal Sequence
- RdRp Gene
- Horizontal Gene Transfer Gene