Skip to main content
Figure 1 | BMC Biology

Figure 1

From: Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state

Figure 1

Identification of Klf5 targets by microarray analysis. (a) qPCR and western blot analysis of Klf5 expression following transfection of siKlf5 and siNS at different time points (12, 24 and 48 hr after siRNA transfection). (b) Expression level of stemness markers was measured 12 and 24 hr after transfection by qPCR and Western blot analysis. All qPCR data are expressed as fold changes relative to mock-transfected cells. Error bars represent standard errors (SD) of triplicates. *P < 0.01. (c and d) Expression of 53 Klf5-target genes measured by real-time PCR in undifferentiated and 4-day (4 d) and 7-day (7 d) differentiated ESCs. Both Klf5-induced genes (c) and Klf5-repressed genes (d) are reported. The results are reported as fold changes relative to undifferentiated cells.

Back to article page

BMC Biology

ISSN: 1741-7007

Contact us