Unraveling bovin phylogeny: accomplishments and challenges
© Bibi and Vrba; licensee BioMed Central Ltd. 2010
Received: 22 April 2010
Accepted: 22 April 2010
Published: 29 April 2010
The phylogenetic systematics of bovin species forms a common basis for studies at multiple scales, from the level of domestication in populations to major cladogenesis. The main big-picture accomplishments of this productive field, including two recent works, one in BMC Genomics, are reviewed with an eye for some of the limitations and challenges impeding progress. See Research article http://www.biomedcentral.com/1471-2164/10/177
One avenue of research that has been continuously providing new information, from Miocene higher clade origins to Holocene population dynamics, has been the analysis of bovin genomes. Deeper and more extensive mining of the genomes of bovin species has seen a consistently improving phylogeny for this clade. A recent study by MacEachern et al.  used 84 autosomal gene sequences from 15 different genes to examine phylogenetic relationships among bovin species and populations, reconstructing geographic divergences and intricate histories of genetic introgression and geographic divergence. Similarly, Decker et al.  used a much expanded genomic data set to investigate phylogenetic relationships from the level of Ruminantia (ruminants, including cattle, antelope, deer, giraffe, and chevrotains) to that of domesticated cattle breeds, including DNA from the extinct Bison priscus. Advances in DNA sequencing techniques have even produced a complete mitochondrial genome from the aurochs (Bos primigenius) , the progenitor of domestic cattle (Bos taurus).
Studies such as these highlight the current focus on unraveling the history of bovin evolution by way of the bovin genome, and the present interest in tracing the history and geography of domestication events. However, two aspects that have seen little progress in the last few years are the phylogenetic assembly of the total bovin clade and the dating of major cladogenetic events within Bovini. Even in the cutting edge studies cited above, little advance is made at the level of major bovin cladogenesis over previous work almost a decade older. From this perspective, the literature of the last years has provided mostly incomplete phylogenies of Bovini dated with inadequate molecular clock estimates. This stems from several factors. First has been the dearth of phylogenetic work on fossil bovin taxa. Second is the regular omission of certain crucial bovin taxa from phylogenetic work. Third is a lack of precision in the phylogenetic terminology used to communicate between paleontological and molecular studies, resulting in the choice of poor references for molecular clock calibration.
We here present our views to highlight some significant gaps and challenges that remain in the field of bovin phylogenetics. Our recommendations also aim to increase the utility of studies for workers of different methodological backgrounds.
Stability in bovin systematics: mission accomplished?
One major remaining conundrum concerns the saola (Pseudoryx nghetinhensis), a little-understood forest antelope from Vietnam and Laos only made known to science since 1993. This species is challenging the definition, composition, and diagnosis of parts of the bovin phylogenetic tree. The saola's small size, simple non-divergent horns, large preorbital fossa (bony depression anterior to the orbital cavity), and simple-shaped teeth make it a very primitive-looking bovid, especially with respect to the large and derived Bovini. It is therefore puzzling that molecular phylogenetic analyses consistently place the saola within Bovini [6, 7]. The exact position and relationship of the saola to the other bovin species is in need of further confirmatory work. Knowing the relationship of this goat-like ungulate to the remainder of Bovini is important, not just to appease curiosity about an enigmatic forest antelope, but because the phylogenetic position of this creature may in fact upset some of the 'stabilized' topology shown in Figure 2 (see also below).
Dating phylogenies: putting the cow before the cart
Phylogenetic studies of fossil Bovini are a crucially missing basis for bovin molecular clock calibrations. Molecular phylogenies are being calibrated using fossil data that are wrong or, at best, highly speculative. For example, reference calibration nodes used in the literature include: the first appearance of Bovidae, the divergence of Bison and Bos, the divergence of Bovina from Bubalina, and the divergence of Bovini from Tragelaphini. However, the reality is that none of the ages of these divergence events is at all well established (Geraads's 1992 work  might be the only extensive phylogenetic analysis of fossil and living bovins to date). The phylogenetic relationships of many fossil Bovini, and, as a result, the evolutionary history of many living Bovini, await more thorough analysis of fossil bovin taxa.
Until better paleontological studies are available, caution and wide margins of error are advised when referring to the ages and phylogenetic placement of fossil bovins for molecular clock calibration. Though commendable efforts have been made in this regard, dates produced from molecular clock estimates will remain very imprecise so long as the fossils used to calibrate these rates are themselves poorly understood phylogenetically. The fossil record of Bovini is already very large and holds great promise for dating the major cladogenetic events within Bovini, so there is every reason for rapid progress in this regard.
From genes to genera: reconciling scales of analysis
The phylogeny of Bovini is being approached from the scale of genes to genera. Studies of the genomes and studies of the fossil record proceed from different methodologies and at different scales, fossils providing a relatively coarse but deep temporal perspective, and molecular work providing a narrow but very highly resolved picture of the modern. Both fields share a common goal, but too often the results of each approach are difficult to reconcile with the other. One limitation to the synthesis of results is the use of limited taxonomic sampling in a phylogenetic analysis. Limited taxonomic representation decreases the reliability and precision of a phylogeny, in turn limiting interpretations on dating, biogeography, and cladogenesis.
Paleontological studies should consider living taxa to the greatest extent they can. This is important considering that workers seeking to calibrate molecular phylogenies are ploughing the literature for information on the timing of origination of crown (that is, extant) clades. Likewise, phylogenetic studies treating living taxa are most informative when they too consider the largest available sample of species. For example, paleontologists investigating the split of Bovina and Bubalina will want to refer to phylogenies that include the entirety of living Bovini to be sure that the node defining the crown clade has been defined to the finest degree possible. Additionally, taxa that are rare or poorly understood have the greatest potential to disrupt 'stable' topologies and alter previous notions, and yet such 'enigmatic' taxa are regularly missing from analyses. An investigation seeking to unravel the relationships among the different clades of Ruminantia cannot afford to omit the Moschidae (musk deer, small ruminants lacking antlers and today restricted to central and northeastern Asia). Any analysis of the Bovini cannot now afford to exclude the saola. It is precisely the fact that such taxa are so disparate in form and restricted in distribution that makes them most interesting for evolutionary reconstruction.
Great progress has been made towards stable systematic classifications in recent years by workers in different fields utilizing different approaches. Continued progress requires an effort to better integrate the different results of the geneticists, ecologists, archaeologists, and paleontologists working on the origins of bovins. Improved communication among workers in different fields will greatly promote the output, precision, and accuracy of results in studies to come.
FB is supported by a National Science Foundation International Research Fellowship Award (#0852975). This work was also supported by the Agence Nationale de la Recherche ANR-09-BLAN-0238.
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