Figure 5

Network synchrony reemerges following prolonged exposure to NA, but can be re-suppressed by cholinergic input. a) Evolution of the Sync Ratio in one experiment (grey; same data after smoothing with a five-point kernel is shown in black). After recording baseline activity for >72 hours (of which the last approximately six hours are shown), NA (20 ?M) was added directly into the MEA dish and the perfusion media reservoir (see Additional file 11: Figure S8 for similar experiments performed with DA). Nineteen hours later, a second bolus of freshly prepared NA was added to the MEA dish. Twenty-four hours after the first NA addition, CCh (20 ?M) was added to the MEA dish and perfusion media reservoir and recording was continued for 40 hours. b) Examples of one-minute raster plots from four stages of the experiment as indicated in the figure (green arrows). c) Evolution of Sync Ratio (averaged over 30 minute intervals) in three similar experiments (blue line is the same experiment as in a). In two of these, a second bolus of freshly prepared NA was added to the MEA dish 19 hours later (indicated as small circles on blue and red traces). Note the very limited effect of this second NA application. CCh, carbachol; DA, dopamine; MEA, multielectrode array; NA, noradrenaline.