- Research article
- Open Access
Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum
© Avezov et al.; licensee BioMed Central. 2015
- Received: 4 November 2014
- Accepted: 23 December 2014
- Published: 10 January 2015
Endoplasmic reticulum (ER) lumenal protein thiol redox balance resists dramatic variation in unfolded protein load imposed by diverse physiological challenges including compromise in the key upstream oxidases. Lumenal calcium depletion, incurred during normal cell signaling, stands out as a notable exception to this resilience, promoting a rapid and reversible shift towards a more reducing poise. Calcium depletion induced ER redox alterations are relevant to physiological conditions associated with calcium signaling, such as the response of pancreatic cells to secretagogues and neuronal activity. The core components of the ER redox machinery are well characterized; however, the molecular basis for the calcium-depletion induced shift in redox balance is presently obscure.
In vitro, the core machinery for generating disulfides, consisting of ERO1 and the oxidizing protein disulfide isomerase, PDI1A, was indifferent to variation in calcium concentration within the physiological range. However, ER calcium depletion in vivo led to a selective 2.5-fold decline in PDI1A mobility, whereas the mobility of the reducing PDI family member, ERdj5 was unaffected. In vivo, fluorescence resonance energy transfer measurements revealed that declining PDI1A mobility correlated with formation of a complex with the abundant ER chaperone calreticulin, whose mobility was also inhibited by calcium depletion and the calcium depletion-mediated reductive shift was attenuated in cells lacking calreticulin. Measurements with purified proteins confirmed that the PDI1A-calreticulin complex dissociated as Ca2+ concentrations approached those normally found in the ER lumen ([Ca2+]K0.5max = 190 μM).
Our findings suggest that selective sequestration of PDI1A in a calcium depletion-mediated complex with the abundant chaperone calreticulin attenuates the effective concentration of this major lumenal thiol oxidant, providing a plausible and simple mechanism for the observed shift in ER lumenal redox poise upon physiological calcium depletion.
- Fluorescence lifetime imaging
- Protein disulfide isomerase
- Endoplasmic reticulum
Cysteine side chains of cytosolic, nuclear and mitochondrial matrix proteins are maintained in their reduced state by a vigorous reductive pathway effected by a large pool of reduced thioredoxins. By contrast, in the lumen of the endoplasmic reticulum (ER) dithiol oxidation and disulfide reduction co-exist. These competing reactions define the redox poise of the ER, which is dominated by the twenty or so members of the protein disulfide isomerase family, the ER counterparts of the thioredoxins (reviewed in ).
Oxidizing members of the protein disulfide isomerase (PDI) family, exemplified by PDI1A , gain disulfides from upstream oxidants, such as ERO1, PRDX4 and VKOR1C and donate them to reduced ER client proteins (reviewed in ). Reducing members of the PDI family, exemplified by ERdj5, [4,5] attack misplaced disulfides on ER clients, and the acquired disulfides are reduced by yet-to-be discovered mechanism(s), re-cycling the reducing PDI to its active state.
Changes to the redox poise of the ER can be tracked by suitably tuned sensors; these are proteins with a metastable disulfide that is kinetically-coupled to the lumenal PDIs and whose redox status influences a measureable outcome, typically fluorescent properties [6,7]. Applying such sensors has revealed that cultured mammalian cells effectively defend their redox poise: neither imposition of unfolded protein load (so-called ER stress), nor manipulations that compromise the upstream oxidases, affect the steady-state disposition of ER localized redox sensors [8,9].
Manipulations that affect ER calcium stores present an intriguing contrast to this theme of stability of ER redox poise. Inhibition of calcium re-uptake into the ER, by targeting the smooth endoplasmic reticulum calcium ATPase (the SERCA) pump with thapsigargin resulted in a reductive shift in ER thiol redox poise. This reductive shift is robust, as it has been observed with redox probes that have different biochemical properties: both the OxyR-YFP fusion known as HyPer , which in the ER is predominantly a thiol redox probe , and roGFPiE [9,12] and by measurements that exploit either redox-dependent shifts in excitation profiles [10,12] or changes in fluorescence lifetime .
In vitro measurements excluded a direct effect of calcium on the redox probe, validating the observed changes as faithful reflections of a reductive shift of the calcium-depleted ER lumen. Furthermore, in a cell culture model of a professional secretory cell - the AR42j pancreatic acinar cell line - physiological mobilization of calcium stores by engagement of the cholecystokinin receptor, which couples to inositol trisphosphate receptor (IP3R) release channels, promoted a similar reductive shift in the ER . The link between calcium release and changes to ER redox thus extends to the physiological range of ER calcium signaling, implying that fluctuations in ER redox accompany activity-dependent oscillations in calcium stores of secretory cells. Here, we report on findings that suggest a molecular mechanism for this pervasive phenomenon.
The core machinery for disulfide bond formation is indifferent to changes in calcium concentrations in the physiological range
roGFPiE is kinetically coupled to the major ER oxidoreductase PDI1A and isolated from the small molecule redox buffer glutathione . To determine if the aforementioned delay in oxidation observed in vivo reflected a direct effect of calcium concentration on the relevant enzymes, we reconstructed the oxidative cascade linking molecular oxygen to reduced roGFPiE in vitro, using purified ERO1 and PDI1A, and established conditions for dependence on the two enzymes. Oxidation of reduced roGFPiE by PDI1A, pre-equilibrated with a glutathione redox buffer, was half-maximal at [PDI1A] K0.5max = 16 μM (Figure 1B), but the oxidation of roGFPiE was unaffected by calcium concentrations over a range of 1 μM to 10 mM (Figure 1C). The concentration-dependence of roGFPiE oxidation on ERO1 (in the presence of saturating concentrations of PDI) was steep, but at ERO1 concentration of 0.5 μM, below the half-maximal concentration (Figure 1D), oxidation of roGFPiE was indifferent to calcium variation over a broad range (Figure 1E).
PDI1A mobility is retarded by ER calcium depletion in vivo
The PDI1A-mCherry fluorescence signal recovered 2.5 times more rapidly in a photobleached patch of ER from untreated cells compared with cells treated with thapsigargin (Figure 2C,D). The recovery of ER localized mCherry fluorescence was unaffected by thapsigargin (Figure 2E,F), as was the recovery of PDI1A-mCherry in cells treated with the glycosylation inhibitor tunicamycin, which induces high levels of ER stress, without affecting calcium (Figure 2G,H and Additional file 1: Figure S1). Importantly, ER calcium depletion retarded PDI1A mobility even in cells exposed to DTT (see Additional file 1: Figure S2), implying that reduced PDI1A is also subject to immobilization, a point we shall return to later.
PDI1A binds calreticulin in the calcium depleted ER
The CRT tertiary structure is modulated by calcium concentration, with enhanced exposure of hydrophobic side-chains at low calcium concentrations . In keeping with this, we observed CRT precipitation with declining concentration of calcium in the buffer. CRT precipitation was prevented by including PDI1A in the solution (see Additional file 1: Figure S3), supporting the FRET measurements noted above and suggesting a role for the calcium-dependent association of the two proteins in maintaining CRT’s solubility.
Association of PDI1A with calreticulin was also observed in DTT-treated cells (see Additional file 1: Figure S4), implying that CRT-binding was not restricted to the oxidized form of PDI1A, which is noted for its higher affinity for unfolded proteins . Furthermore, complex formation with CRT did not affect PDI1A’s ability to catalyze the oxidation of reduced roGFPiE in vitro (see Additional file 1: Figure S5). These negative results leave altered PDI1A mobility as the notable correlate of calcium depletion.
Genetic evidence for the importance of CRT to the reductive shift in the calcium-depleted ER was provided by comparison of roGFPiE fluorescent lifetime in wild type and CRT-deficient mouse embryonic fibroblasts. The absence of CRT had no effect on the basal redox state of the probe or on its redox state in cells exposed to DTT. However, the reductive shift observed upon exposure of the wild type cells to the ER calcium depleting agent thapsigargin (reflected in a shortening of lifetime from 1,947 ± 28 to 1,701 ± 107 ps), was blunted in the CRT-deficient cells (shortening of lifetime from 1,946 ± 53 to 1,869 ± 38 ps) (Figure 7D) indicating a non-redundant role for CRT in promoting the reductive shift in the calcium depleted ER.
The reductive shift in ER protein thiol redox poise that follows lumenal calcium depletion is shown here to correlate with a kinetic defect in the rate of recovery of disulfides following a reductive pulse and its washout. However, the core enzymatic machinery that produces disulfides in the ER and conveys them to reduced protein substrates is unresponsive to calcium in vitro. In vivo, however, the mobility of the prototypical oxidizing PDI, PDI1A, is lowered by calcium depletion and this correlates with the formation of a calcium-suppressible complex between PDI1A and the ER chaperone CRT, both in vitro and in vivo.
CRT is well suited to serve as a calcium sensor in the interaction with PDI1A, as it has been noted to change its tertiary structure in response to changes in calcium concentration, with increased exposure of hydrophobic surface as calcium concentration decreases . PDI1A and CRT are both abundant ER proteins and their C-termini are rich in acidic residues that could mediate calcium responsiveness of complex formation. However, deletion of the C-terminal regions (that are not essential to structural and functional integrity) proved insufficient to abolish the calcium depletion-mediated interaction, frustrating our efforts at gross deletional mapping of the calcium-dependent interaction between them.
Association with CRT does not inactivate PDI1A in vitro, and neither complex formation, nor in vivo mobility exhibit measureable specificity for the oxidized form of PDI1A. Rather, our findings fit best with non-selective sequestration of both reduced and oxidized PDI1A in lower mobility complex(es) in the calcium depleted ER. The FRAP traces of PDI1A do not resolve into a simple model of only two pools (free and CRT-bound), thus the PDI1A-CRT complex may be heterogeneous or PDI1A may interact with other proteins too, forming an ensemble of complexes that diffuse slowly in the calcium-depleted ER. Indeed, the 2.5 fold decrease in PDI1A upon calcium depletion fits with a complex larger than merely a PDI1A-CRT dimer. Unfortunately, instability of the PDI1A-CRT complex has frustrated our efforts at isolation and biochemical characterization. Despite these limitations to our understanding, the mechanism(s) promoting a reductive shift in the calcium depleted ER are defined with considerable specificity. That a reductive shift is not observed in conditions associated with markedly elevated levels of unfolded protein stress in the ER (for example, in tunicamycin-treated cells ) argues that promiscuous effects of calcium depletion on the handling of secreted proteins are unlikely to contribute. The evidence for specificity is further buttressed by the significant effacement of the reductive shift in cells lacking CRT.
What might be the physiological significance of the reductive shift of the calcium-depleted ER? It is best to begin by acknowledging the possibility that redox effects may not be under positive selection but rather reflect a consequence of a role for PDI1A in maintaining CRT’s solubility through physiological fluctuations in [Ca2+]ER. However, it is also intriguing to consider this question in the context of physiological circumstances associated with calcium excursions into the range that effect PDI1A-CRT association and ER reduction. Such excursions are noted in secretory cells responding to secretagogues and are associated with physiological activation of the unfolded protein response . The reductive shift in the ER may contribute to such activation, transiently inhibiting protein synthesis, through rapidly reversible protein kinase RNA-like endoplasmic reticulum kinase (PERK)-mediated eIF2 phosphorylation . Transient inhibition of energetically-costly protein synthesis may be especially important in insulin-producing beta cells, in which coupling of insulin secretion to blood glucose requires high levels of intracellular ATP . Furthermore, coupling secretory stimuli (which are transient) to periodic activation of UPR target genes would have enduring effects on ER size and on the long-term capacity to produce secreted products. The evolution of an ER that is more reducing under conditions of ER calcium store depletion would promote fitness by coupling the action of calcium-mobilizing secretagogues to a UPR-mediated transient attenuation of protein synthesis followed by long-term growth of the ER.
Transient reductive departures from the normally oxidizing poise of the ER may provide other benefits to cells. Shifting the balance from the oxidizing PDI1A to the reducing ERdj5 is predicted to favor degradation of disulfide bonded misfolded proteins . Thus, transient cycles of reduction may be helpful in periodically purging the ER of recalcitrant misfolded proteins, while maintaining ER redox at levels optimized for oxidative folding most of the time. Coupling such reductive shifts to PERK-mediated attenuation of protein synthesis would lessen their impact on oxidative folding of newly-synthesized proteins, while promoting the degradation of refuse.
A functional link between ER redox and calcium homeostasis is suggested by the phenotype of mice lacking ERO1 genes. Cells explanted from such animals have no steady state defect in ER thiol redox poise, but recover sluggishly from reductive excursions . The mice are superficially indistinguishable from wild type; however, calcium handling by cardiomyocytes is grossly impaired, supporting a role for rapid kinetics of disulfide recovery in ER calcium release and re-uptake .
Calcium handling by the ER is subject to redox regulation. Levels of ERO1 activity modulate calcium fluxes in and out of the ER . Subtype 1 of the IP3 receptor, which conducts lumenal calcium to the cytosol in response to activation of membrane receptors, is inhibited by the formation of a mixed disulfide between cysteine residues on its third lumenal loop and reduced ERp44 , a reducing PDI family member. By contrast, the broadly-expressed calcium reuptake pump, SERCA2b is inhibited by the formation of a disulfide with the oxidizing PDI family member ERp57 . As disulfide exchange between PDI family members is pervasive, these observations suggest that a more reduced ER poise would favor re-uptake of calcium into the ER and disfavor its release. It follows that immobilization of the oxidizing PDI1A, which can transfer disulfides to ERp57 , and continued mobility of the reducing ERdj5 would favor restoration of calcium stores to the calcium-depleted ER closing a homeostatic loop.
The reductive shift in ER redox poise, observed at physiological ER calcium store depletion, is an unusual exception to a landscape dominated by stability of ER redox balance. Direct calcium responsiveness of the core machinery for oxidizing ER client protein thiols has been dismissed by our studies. However a remarkable correlation between lumenal calcium depletion and lowered mobility of the oxidizing PDI1A in the ER lumen was observed. This, in turn, is plausibly explained by formation of a calcium responsive complex between PDI1A and the abundant ER chaperone CRT with the effect of sequestering a key component of the oxidizing limb of the ER (Figure 8). The consequent attenuation of client protein oxidation accompanies physiological signals that specify secretion, thus delineating a hitherto unanticipated link between secretagogue action and protein folding homeostasis in the ER.
Additional file 2: Table S1 lists the plasmids used, their lab names, description, published reference and a notation of their appearance in the figures.
Protein purification and in vitro enzymatic assays
Human PDI (PDIA1 18–508), mouse ERO1L and roGFPiE were expressed in the E. coli BL21 (DE3) strain and purified with Ni-NTA affinity chromatography or glutathione affinity chromatography as previously described [9,27]. Time-dependent changes in redox of roGFPiE, in the presence of ERO1 and PDI1, were measured as described [9,28].
Transfections, immunoblotting, immunofluorescence and cell culture
HEK 293 T, COS7 and AR42j cells were obtained from the (ATCC, Teddington, Middlesex, UK). Mouse embryonic fibroblasts lacking CRT and their wild type counterparts were a gift of Marek Michalak (University of Alberta, Alberta, Canada) . Cells were cultured in DMEM (SIgma, Gillingham, Dorset, UK), supplemented with 10% fetal calf serum. Transfections were performed using the Neon Transfection System (Invitrogen, Paisley, UK). For shRNA knock down, stably transfected HEK293T cells were selected in puromycin (2 μg/ml). Cells from confluent 100-mm dishes were washed in phosphate-buffered saline (PBS), lysed in 0.5% Triton X-100, 150 mM NaCl, 20 mM Hepes, pH 7.4, and protease inhibitors. Proteins were resolved by 12% SDS-PAGE and blotted with a mouse monoclonal anti-PDI (1D3 Assay Designs, Exeter, UK).
C/EBP Homologous Protein (CHOP) immune-reactivity was detected by immunofluorescence as described . To localize PDI-mCherry and ERdj5-mCherry the proteins were visualized alongside BiP in 2% paraformaldehyde fixed cells using a chicken polyclonal antibody raised in hen against full-length bacterially-expressed hamster BiP , anti-chicken DyLight488 conjugated immunoglobulin G (IgG) was used as a secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).
Protein purification and enzymatic assays
For in vitro assays, human PDI (PDIA1 18–508, a gift of Colin Thorpe, University of Delaware, Newark, DE, USA), mouse Ero1α and roGFPiE were expressed in E. coli BL21 (D3) strain, purified with Ni-NTA affinity chromatography, and dialyzed into the reaction buffer, and reduced by incubation with 20 mM of DTT, and then buffer exchanged on a PD-10 gel filtration column (GE Healthcare, Little Chalfont, Buckinghamshire, UK) . For kinetic assay, reduced PDI (0 to 150 μM) was equilibrated in degased 100 mM HEPES pH 7.4, 150 mM NaCl buffer containing an excess of a combination of reduced and oxidized glutathione (4 mM, Sigma) for one hour at room temperature, then added simultaneously to all the samples in the experiment containing 2 μM reduced roGFPiE. For the assessment of Ero1 activity the enzyme (0 to 15 μM) was added to reduced PDI (30 μM) and roGFPiE (2 μM). The ratio of fluorescence emission at 520 nm following sequential excitation at 395 and 470 nm was measured using EnSpire® Multimode Plate Readers (PerkinElmer, Waltham, MA, USA).
To assess the calcium dependence of PDI-mCherry/CRT-GFP interaction in vitro the proteins were equilibrated in 50 mM HEPES pH = 7.2, 100 mM KCl buffer containing 2 mM CaCl2, then increasing amounts of ethyleneglycoltetraacetic acid (EGTA) were introduced to yield a range of free Ca2+ concentrations (8 to 2,000 μM, calculated using Maxchelator, Stanford University, Palo Alto, CA, USA). FRET signal was detected measuring the ratio between the emission at 630 nm and 520 nm, both excited at 485 nm.
FLIM and intensity-based confocal microscopy and image analysis
A Zeiss 510 Meta laser scanning confocal system with Plan-Apochromat ×63 oil immersion lens (NA = 1.6) was used to acquire intensity based fluorescence microscopy images of cells fixed with 2% paraformaldehyde PBS. Images were analyzed using ImageJ with JACoP (National Institutes of Health, Bethesda, MD, USA) to assess colocalization.
FLIM experiments were performed on a modified version of a previously-described laser scanning multiparametric imaging system , coupled to a microscope incubator, maintaining standard tissue culture conditions (Okolab, Pozzuoli, NA, Italy), using a pulsed (sub 10 ps, 20 MHz) supercontinuum (430 to 2,000 nm) light source (SC 450, Fianium Ltd., Southampton, UK). Desired excitation wavelength (typically 470 nm for FLIM of GFP variants and 440 nm for D1ER cameleon FRET) was tuned using an acousto-optic tunable filter (AA Opto-electronic AOTFnC-VIS, Orsay, France). Desired emission was collected using 510/42 and 470 to 490 nm bandpass filters for GFP variants and D1ER cameleon accordingly and detected by a fast photomultiplier tube (PMC-100, Becker & Hickl GmbH, Berlin, Germany). Lifetimes for each pixel were recorded using time correlated single photon counting (TCSPC) circuitry (SPC-830, Becker & Hickl GmbH), keeping count rates below 1% of the laser repetition rate to prevent pulse pile-up. Images were acquired over 20 to 60 seconds, with a typical flow rate of 5 × 104 photons sec−1 , while detectors speed saturation (pile up effect) is not observed below 106 photons sec−1 in this instrument. The data were processed using SPCImage (Becker & Hickl GmbH) fitting the time correlated photon count data obtained for each pixel of the image to a mono-exponential decay function, yielding a value for lifetime on the pico-second scale.
After filtering out autofluorescence (by excluding pixels with a fluorescence lifetime that is out of range of the roGFP probes, that is, longer than 2,800 ps), mean fluorescence lifetime of single cells was established. The value obtained represented the redox as sensed by roGFPiE, FRET efficiency of D1ER cameleon (inversely proportional to Ca2+ concentration) or of GFP/mCherry. Each data point is constituted by the average and SD of measurements from at least 10 cells. For GFP/mCherry in vivo FLIM FRET, cells with sufficient expression of mCherry were selected based on the signal obtained from excitation at 550 nm and 612/35 emission. FRET efficiency was calculated using the equation: EFRET = 1 ‐ τDA/ τD, where τD and τDA are the lifetime values of the FRET donor in the absence or presence of an acceptor, respectively. For calculations of interacting fractions of CRT-GFP, lifetime of GFP-mCherry fusion was assumed to represent 100% interacting fraction .
For FRAP analysis of mCherry and GFP fused proteins a series of 60 to 140 images with two second intervals were acquired. Bleaching in a selected region of interest (ROI) was introduced at image 10, using 100% laser power (488 nm for GFP fused proteins and 514/543 nm for mCherry fused proteins, 100 to 300 iterations). Single recovery halftime (t1/2) was calculated using the FRAP macro of ZEN software (Carl Zeiss Microimaging, Oberkochen, Germany).
The FRET based, ER-optimized D1ER cameleon , was used for ER calcium imaging by FLIM (exploiting fluorescence lifetime shortening effect of FRET on the emission of the cyan fluorescent protein segment of D1ER cameleon). Concentration of ER calcium was calculated from the fluorescence lifetime value based on the equation: [Ca2 +] = (kd * (τmax–τ)/(τ ‐ τmin))1/h, where kd is the apparent dissociation constant of the probe and h is Hill’s coefficient (197 μM and 0.87, respectively, measured in vivo ), τmax and τmin are the highest and the lowest values of the probes’ lifetime measured in permeablized cells exposed to buffers with 0 or saturating calcium, respectively (full method will be published separately).
We are grateful to James Remington (University of Oregon) for the gift of roGFP plasmids, Colin Thorpe (University of Delaware) for the gift of PDI1A plasmids and Marek Michalak (University of Alberta, Canada) for the calreticulin knockout MEF cell line.
Supported by grants from the Wellcome Trust (Wellcome 084812/Z/08/Z) the European Commission (EU FP7 Beta-Bat No: 277713) and Fundação para a Ciência e Tecnologia, Portugal (PTDC/QUI-BIQ/119677/2010). DR is a Wellcome Trust Principal Research Fellow.
- Hatahet F, Ruddock LW. Protein disulfide isomerase: a critical evaluation of its function in disulfide bond formation. Antioxid Redox Signal. 2009;11:2807–50.PubMedView ArticleGoogle Scholar
- Givol D, Delorenzo F, Goldberger RF, Anfinsen CB. Disulfide interchange and the three-dimensional structure of proteins. Proc Natl Acad Sci U S A. 1965;53:676–84.PubMed CentralPubMedView ArticleGoogle Scholar
- Bulleid NJ, Ellgaard L. Multiple ways to make disulfides. Trends Biochem Sci. 2011;36:485–92.PubMedView ArticleGoogle Scholar
- Ushioda R, Hoseki J, Araki K, Jansen G, Thomas DY, Nagata K. ERdj5 is required as a disulfide reductase for degradation of misfolded proteins in the ER. Science. 2008;321:569–72.PubMedView ArticleGoogle Scholar
- Oka OB, Pringle MA, Schopp IM, Braakman I, Bulleid NJ. ERdj5 is the ER reductase that catalyzes the removal of non-native disulfides and correct folding of the LDL receptor. Mol Cell. 2013;50:793–804.PubMed CentralPubMedView ArticleGoogle Scholar
- Lohman JR, Remington SJ. Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments. Biochemistry. 2008;47:8678–88.PubMedView ArticleGoogle Scholar
- Birk J, Ramming T, Odermatt A, Appenzeller-Herzog C. Green fluorescent protein-based monitoring of endoplasmic reticulum redox poise. Front Genet. 2013;4:108.PubMed CentralPubMedView ArticleGoogle Scholar
- van Lith M, Tiwari S, Pediani J, Milligan G, Bulleid NJ. Real-time monitoring of redox changes in the mammalian endoplasmic reticulum. J Cell Sci. 2011;124:2349–56.PubMed CentralPubMedView ArticleGoogle Scholar
- Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, Melo EP, et al. Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox. J Cell Biol. 2013;201:337–49.PubMed CentralPubMedView ArticleGoogle Scholar
- Enyedi B, Varnai P, Geiszt M. Redox state of the endoplasmic reticulum is controlled by Ero1L-alpha and intraluminal calcium. Antioxid Redox Signal. 2010;13:721–9.PubMedView ArticleGoogle Scholar
- Mehmeti I, Lortz S, Lenzen S. The H2O2-sensitive HyPer protein targeted to the endoplasmic reticulum as a mirror of the oxidizing thiol-disulfide milieu. Free Radic Biol Med. 2012;53:1451–8.PubMedView ArticleGoogle Scholar
- Birk J, Meyer M, Aller I, Hansen HG, Odermatt A, Dick TP, et al. Endoplasmic reticulum: reduced and oxidized glutathione revisited. J Cell Sci. 2013;126:1604–17.PubMedView ArticleGoogle Scholar
- Corbett EF, Oikawa K, Francois P, Tessier DC, Kay C, Bergeron JJ, et al. Ca2+ regulation of interactions between endoplasmic reticulum chaperones. J Biol Chem. 1999;274:6203–11.PubMedView ArticleGoogle Scholar
- Wijeyesakere SJ, Gafni AA, Raghavan M. Calreticulin is a thermostable protein with distinct structural responses to different divalent cation environments. J Biol Chem. 2011;286:8771–85.PubMed CentralPubMedView ArticleGoogle Scholar
- Berezin MY, Achilefu S. Fluorescence lifetime measurements and biological imaging. Chem Rev. 2010;110:2641–84.PubMed CentralPubMedView ArticleGoogle Scholar
- Palmer AE, Jin C, Reed JC, Tsien RY. Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004;101:17404–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang C, Yu J, Huo L, Wang L, Feng W, Wang CC. Human protein-disulfide isomerase is a redox-regulated chaperone activated by oxidation of domain a’. J Biol Chem. 2012;287:1139–49.PubMed CentralPubMedView ArticleGoogle Scholar
- Hagiwara M, Maegawa K, Suzuki M, Ushioda R, Araki K, Matsumoto Y, et al. Structural basis of an ERAD pathway mediated by the ER-resident protein disulfide reductase ERdj5. Mol Cell. 2011;41:432–44.PubMedView ArticleGoogle Scholar
- Kubisch CH, Logsdon CD. Secretagogues differentially activate endoplasmic reticulum stress responses in pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol. 2007;292:G1804–12.PubMedView ArticleGoogle Scholar
- Harding H, Zhang Y, Ron D. Translation and protein folding are coupled by an endoplasmic reticulum resident kinase. Nature. 1999;397:271–4.PubMedView ArticleGoogle Scholar
- Ashcroft SJ, Weerasinghe LC, Randle PJ. Interrelationship of islet metabolism, adenosine triphosphate content and insulin release. Biochem J. 1973;132:223–31.PubMed CentralPubMedGoogle Scholar
- Chin KT, Kang G, Qu J, Gardner LB, Coetzee WA, Zito E, et al. The sarcoplasmic reticulum luminal thiol oxidase ERO1 regulates cardiomyocyte excitation-coupled calcium release and response to hemodynamic load. FASEB J. 2011;25:2583–91.PubMed CentralPubMedView ArticleGoogle Scholar
- Anelli T, Bergamelli L, Margittai E, Rimessi A, Fagioli C, Malgaroli A, et al. Ero1alpha regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM). Antioxid Redox Signal. 2012;16:1077–87.PubMedView ArticleGoogle Scholar
- Higo T, Hattori M, Nakamura T, Natsume T, Michikawa T, Mikoshiba K. Subtype-specific and ER lumenal environment-dependent regulation of inositol 1,4,5-trisphosphate receptor type 1 by ERp44. Cell. 2005;120:85–98.PubMedView ArticleGoogle Scholar
- Li Y, Camacho P. Ca2 + −dependent redox modulation of SERCA 2b by ERp57. J Cell Biol. 2004;164:35–46.PubMed CentralPubMedView ArticleGoogle Scholar
- Araki K, Iemura S, Kamiya Y, Ron D, Kato K, Natsume T, et al. Ero1-alpha and PDIs constitute a hierarchical electron transfer network of endoplasmic reticulum oxidoreductases. J Cell Biol. 2013;202:861–74.PubMed CentralPubMedView ArticleGoogle Scholar
- Blais JD, Chin KT, Zito E, Zhang Y, Heldman N, Harding HP, et al. A small molecule inhibitor of endoplasmic reticulum oxidation 1 (ERO1) with selectively reversible thiol reactivity. J Biol Chem. 2010;285:20993–1003.PubMed CentralPubMedView ArticleGoogle Scholar
- Tsunoda S, Avezov E, Zyryanova A, Konno T, Mendes-Silva L, Pinho Melo E, et al. Intact protein folding in the glutathione-depleted endoplasmic reticulum implicates alternative protein thiol reductants. Elife. 2014;3:e03421.PubMed CentralPubMedView ArticleGoogle Scholar
- Mesaeli N, Nakamura K, Zvaritch E, Dickie P, Dziak E, Krause KH, et al. Calreticulin is essential for cardiac development. J Cell Biol. 1999;144:857–68.PubMed CentralPubMedView ArticleGoogle Scholar
- Ron D, Habener JF. CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant negative inhibitor of gene transcription. Gene Dev. 1992;6:439–53.PubMedView ArticleGoogle Scholar
- Frank JH, Elder AD, Swartling J, Venkitaraman AR, Jeyasekharan AD, Kaminski CF. A white light confocal microscope for spectrally resolved multidimensional imaging. J Microsc. 2007;227:203–15.PubMedView ArticleGoogle Scholar
- Lleres D, Swift S, Lamond AI. Detecting protein-protein interactions in vivo with FRET using multiphoton fluorescence lifetime imaging microscopy (FLIM). Curr Protoc Cytom. 2007;Chapter 12:Unit12 10.Google Scholar
- Esfandiarei M, Fameli N, Choi YY, Tehrani AY, Hoskins JG, van Breemen C. Waves of calcium depletion in the sarcoplasmic reticulum of vascular smooth muscle cells: an inside view of spatiotemporal Ca2+ regulation. PLoS One. 2013;8:e55333.PubMed CentralPubMedView ArticleGoogle Scholar
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