- Open Access
In vivo studies of the Drosophila insulator factor CTCF reach a Catch 22
© Karch. 2015
- Published: 2 September 2015
Mutations in the proteins that bind insulator DNA elements that define the boundaries of chromatin domains can give morphogenetic readouts in Drosophila, as recently reported in BMC Biology by Bonchuk et al. in the Georgiev laboratory. But disentangling the effects on the phenotype may not be simple.
See research article: http://www.biomedcentral.com/1741-7007/13/63
- Gaga Factor
- Homeotic Transformation
- Insulator Protein
- CTCF Protein
- C2H2 Zinc Finger Domain
Insulator DNA elements, also referred to with the more generic term boundaries, are important architectural components of the genome and nuclear organization that are conserved through evolution. They play an active part in gene regulation by binding proteins that interact to organize chromatin into loops thought to define autonomous chromatin domains (reviewed in [1, 2]), thereby modulating the activities of enhancers and silencers in specific tissues and/or developmental stages. They were first identified in Drosophila on the basis of their enhancer-promoter blocking activity, an activity thought to restrict the promiscuous activity of enhancers. Insulators also protect transgenes from genomic position effects, by establishing independent functional domains within the chromosomes (for review see ). Bonchunk et al.  now report a combination of biochemical and genetic experiments with CTCF, the most conserved DNA-binding insulator protein, in Drosophila. Thanks to the powerful Drosophila molecular genetic toolbox, the new biochemical properties of CTCF described in the paper are directly assessed in vivo. I believe that these new in vivo results reach a Catch 22 situation that complicates the interpretation and that is the subject of this commentary.
The proteins that associate with boundaries/insulators fall into two categories. The first group encompasses the DNA-binding proteins. Here most of what we know comes from studies in Drosophila where nine of them have been identified. They include in the order of their identification Su(Hw), BEAF-32, the GAGA factor, Zw5, dCTCF, the Elba1/Elba2/Elba3 protein complex, Pita and ZIPIC, and finally the Ibf1 and Ibf2 proteins (see original citations in ). Unexpectedly, the situation is apparently much simpler in vertebrates where only CTCF has been identified as an insulator DNA binding protein (for review see for example ; the other fly proteins do not have obvious orthologs in vertebrates). ChiP on ChiP/seq analysis revealed the existence of multiple genomic sites with distinct combinations of these insulator DNA-binding factors (see for example  and references in ).
The second group of proteins that associate with Drosophila insulators consists of CP190, and multiple protein isoforms encoded by the locus mod(mdg4) (see [1, 5, 6] for references to original papers). Cp190 and Mod(mdg4) do not bind directly to DNA but they can mediate homotypic and heterotypic protein-protein interactions via their BTB/POZ domains. Interestingly these two proteins are associated with most boundary/insulator elements within the genome, and CP190 was proposed to play the role of universal ‘glue’ that mediates long-distance interactions between insulator elements of different classes, thereby generating chromatin loops . In vertebrates, the glue component associating with CTCF is thought to be the cohesin complex .
Vertebrate and Drosophila CTCF are well conserved in their 12 C2H2 zinc finger domains, and bind similar sequences . In contrast their N- and C-terminal domains differ, probably reflecting their different interaction partners (see above). Using various biochemical methods, Bonchuk et al.  find a multimerization domain in the N-terminus of dCTCF that likely mediates the formation of tetrameric complexes. At the C-terminal domain a module encompassing 200 amino acids of dCTCF mediates interaction with the N-terminal BTB domain of CP190.
In trying to study the functional significance of these two domains in vivo, the authors made interesting observations. First they reassessed the phenotype of various CTCF alleles available in the literature  and provide compelling evidence that a null mutation is able to develop to adulthood. This does not necessarily imply that dCTCF is dispensable for development however. Many essential genes are heavily transcribed during oogenesis and their transcripts are transferred to the oocyte, allowing development to proceed until adulthood thanks to the maternal store. The more surprising observation is that normal oogenesis can proceed in homozygous null dCTCF females, which lay eggs able to undergo normal development if they are fertilized by a sperm carrying a functional dCTCF gene.
Among various hypotheses discussed in the article I like the idea that fly insulators are the targets of multiple DNA binding proteins, allowing some functional redundancy: in this view the lack of one insulator protein can be compensated for by an alternative one. Redundancy may also explain why a CTCF protein lacking the CP190 interaction domain is almost as effective as the wild-type form of CTCF in rescuing the dCTCF mutant phenotype. Indeed CP190 can be recruited by an alternative insulator protein such as Su(Hw), Ibf1/Ibf2,, Pita or ZIPIC (see ref 3 for references to the original papers). In contrast, a CTCF protein lacking the multimerization domain is less efficient in rescuing the dCTCF mutant phenotype, opening up the possibility that CTCF multimer formation is important for insulator binding.
While homozygous null dCTCF flies can develop to adulthood they do not look entirely wild type, harboring thin bristles, partially folded wings and homeotic transformations of the posterior abdominal segments (see below). The question then arises of whether the phenotypes observed can shed some light on insulator or boundary functions. The question is not trivial to address because nearly all insulators/boundaries have been identified with reporter gene assays, and thus outside their native chromosomal context. An answer to this problem comes from a special class of gain-of-function (GOF) mutations in the bithorax complex of hox genes (BX-C) that cause homeotic transformations of abdominal segments. These dominant mutations result from the deletions of boundaries. Very interestingly, dCTCF homozygotes show homeotic transformations of the posterior abdominal segments that are specified by the Abd-B gene of the BX-C, suggesting that the boundary functions in the BX-C are compromised in dCTCF mutant background.
Additional boundaries flank the regulatory domains that control abd-A in the more anterior abdominal segments A2-A4. Interestingly, all BX-C boundaries (with the exception of Fab-7) contain binding sites for dCTCF . In a simple world, mutation in dCTCF would be expected to interfere with all BX-C boundaries and result in an all out situation in which all regulatory domains open up simultaneously. In the case of Abd-B, an A8-like expression pattern should appear from A5, resulting in a posterior-oriented transformation of segment identity (towards A8). The reality is more complex, with dCTCF null homozygous flies harboring a mixture of GOF and LOF phenotypes in the identities of their posterior abdominal segments. Bonchunk et al. favor the idea that this mixture of GOF and LOF phenotypes is a readout of crippled boundaries that would either fail to block activation spreading (GOF) or silencing spreading (LOF).
But this mixture of GOF and LOF transformation of segment identity may equally reflect a difficulty of interpretation inherent in the multifarious nature of CTCF, which can bind both to the insulators and to the Abd-B promoter. Thus in a dCTCF mutant background, a loss of activity of the Abd-B promoter may also occur in addition to the crippled boundaries. In as much as Abd-B activity is required for the readout of boundary mutations, one reaches a sort of Catch-22 situation. Some cells may have enough CTCF to ensure proper Abd-B promoter activity to display the GOF phenotype, while some other cells may shut down their Abd-B promoter, leading to a LOF phenotype. A similar scenario could explain conflicting observations made with the GAGA factor, which according to different authors is classified either as trithorax-group genes (trx-G, activators ) or as Polycomb-group genes (Pc-G, repressors . On one hand, the Pc-G activity of the GAGA factor was concluded from experiments performed ectopically using a reporter gene assay. On the other hand, the trx-G activity of GAGA was based on the LOF homeotic phenotype observed in the context of a GAGA mutant background. As for CTCF, GAGA sites also decorate the Hox gene promoters. A compromised Polycomb repression remains ineffective if the promoter for its readout is also inactive.
Somehow multi-tasking proteins, such as CTCF or GAGA, that bind at both regulatory elements and at the target promoter controlled by these regulatory elements, are difficult to study by genetics. Genes controlling development are often expressed in very tight spatio-temporal patterns that are controlled by complex and extensive cis regulatory regions. Any defects in the organization of these control regions may have important effects on the expression pattern and lead to deleterious effects. Having the factors organizing the activity of these regulatory regions also involved in promoter activity may have been adapted though evolution to buffer the consequences of mis-regulation.
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