Q&A: How do plants respond to ethylene and what is its importance?
© Chang. 2016
Published: 27 January 2016
Ethylene gas is a major plant hormone that influences diverse processes in plant growth, development and stress responses throughout the plant life cycle. Responses to ethylene, such as fruit ripening, are significant to agriculture. The core molecular elements of the ethylene-signaling pathway have been uncovered, revealing a unique pathway that is negatively regulated. Practical applications of this knowledge can lead to substantial improvements in agriculture.
What is the plant hormone ethylene?
The simple hydrocarbon ethylene (C2H4) is a tiny gaseous molecule of great significance. In addition to being the most widely produced organic compound in the world (used in manufacturing numerous products such as rubber, plastics, paints, detergents and toys), ethylene is a major hormone in plant biology. This volatile molecule mediates many complex aspects of plant growth, development and survival throughout the plant life cycle, including seed germination, root development, shoot and root growth, formation of adventitious roots, abscission of leaves and fruits, flowering, sex determination, and senescence of flowers and leaves [1, 2]. Ethylene also mediates adaptive responses to a variety of stresses, such as drought, flooding, pathogen attack and high salinity. During flooding, for instance, ethylene induces the formation of aerenchyma tissue (consisting of air-filled cavities) for oxygenation. Ethylene is best known, however, for its essential role in the ripening of climacteric fruits, such as tomatoes, bananas, pears and apples. Placing a ripe banana in a paper bag containing unripe avocados, for instance, will hasten ripening of the avocados due to the accumulation of ethylene produced by the banana.
Why is ethylene important to agriculture?
Controlling ethylene responses is a major commercial enterprise due to the wide-ranging effects of ethylene on plants of agronomic and horticultural value . Interestingly, responses to ethylene can be either harmful or desirable, depending on the species, developmental stage and concentration of ethylene. Too much ethylene, for example, can result in the spoilage of produce, as aptly conveyed by the saying “one bad apple spoils the whole bunch”. Costly methods are therefore employed to prevent the spoilage of fruits, vegetables and flowers during their transport and storage. These methods include the use of adsorbents and scrubbers to remove external ethylene, the use of chemical inhibitors to prevent ethylene biosynthesis and the use of chemical inhibitors (e.g., SmartFresh) to prevent ethylene signal transduction. Blocking ethylene perception during crop growth can also prevent abscission of leaves and flowers and yellowing of vegetables. On the other hand, ethylene is intentionally applied in situations where ethylene responses are desirable. Fruit ripening is typically induced pre- or post-harvest using ethylene or ethephon, which is a commercial liquid formulation of ethylene. Ethephon is also sprayed on pineapple plants to induce flowering and sprayed on wheat plants to prevent lodging (bending over).
How was the ethylene hormone discovered?
Interestingly, the discovery of ethylene as a plant hormone came about due to the unintended presence of ethylene in the environment [1, 3]. In the 1800s, illuminating gas (coal gas) was widely used for lighting, and its leakage from gas lines was known to cause extensive damage to plants, such as the defoliation of trees around streetlamps. Near the end of the 1800s, Dimitry Neljubow observed that etiolated pea seedlings exhibited a peculiar growth (consisting of a shortened and thickened epicotyl and horizontal bending) due to leaking illuminating gas in his laboratory. Neljubow determined that ethylene was the biologically active component of illuminating gas. This finding led to numerous studies on the wide-ranging effects of ethylene. In 1934, Richard Gane discovered that plants synthesize ethylene; the correlation of ethylene biosynthesis with biological activity was a major step toward convincing researchers that a gas could be a plant hormone. In fact, ethylene was the first gaseous signaling molecule to be identified in any organism .
Is there anything different about a gaseous hormone?
Ethylene is different from non-gaseous hormones in several ways. Ethylene moves within the plant by diffusion and is thought to be synthesized at or near its site of action, similar to the gaseous signal nitric oxide in mammals. Because ethylene can diffuse across membranes into nearby cells, there is no requirement for transporter proteins to deliver ethylene to target cells, and, in fact, no such transporters have been identified, though there is transport of the immediate precursor to ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC) . Ethylene is also not known to be conjugated or broken down for storage or deactivation; ethylene simply diffuses away from the plant. While managing a gaseous hormone is simpler for plants, it is more complicated for researchers. Ethylene experiments are generally carried out in contained environments, such as airtight chambers, although in some situations this can be circumvented by treating plants with ACC instead of ethylene.
How do plants synthesize ethylene?
How much ethylene is needed to trigger a response in plants?
Ethylene is biologically active at very low concentrations of around 0.01 to 1.0 part per million (ppm). Lower or higher sensitivities have been observed depending on the species and the response. Some climacteric fruits, such as tomatoes and apples, can generate tens of ppm of ethylene. It is worth noting here that ethylene is a byproduct of partial combustion of organic fuels and is present, therefore, in the atmosphere due to such things as forest fires, volcanic eruptions and car exhaust . A study in 1973 detected up to 0.7 ppm of ethylene around the Beltway (the highway that circles Washington DC and the University of Maryland), and these levels had a harmful impact on the surrounding vegetation .
Is ethylene harmful to humans?
Metazoans lack ethylene receptor homologs and do not perceive and respond to ethylene as plants do. High concentrations of ethylene (>1000 ppm) can, however, cause dizziness or light-headedness. For several decades in the 1900s, ethylene was used as a general anesthetic . In ancient Greece, ethylene emanating from geologic faults beneath the Oracle of Delphi’s underground chamber may have been responsible for the oracles’ trance-like states and prophetic hallucinations . The greatest danger in working with pure ethylene is the risk of explosion, because ethylene is a flammable gas. However, there is far too little ethylene in a tomato for it to explode!
How was the ethylene-signaling pathway determined?
What does the ethylene-signaling pathway look like?
What type of receptor is the ethylene receptor?
The ethylene receptors form disulfide-linked dimers, and each dimer is capable of binding a single ethylene molecule  with the help of a copper ion cofactor . The dimers reside in clusters at the ER membrane where they interact with downstream proteins in the pathway [16, 17]. The GAF domain, usually known for binding small molecules, facilitates protein–protein interactions between ethylene receptor monomers as well as between isomers [16, 17].
How do the ethylene receptors signal?
This is a good question. Currently this is best understood at the genetic level. From genetic analyses, we know that the receptors are negative regulators of ethylene responses. In other words, ethylene responses are repressed by ethylene receptor signaling . This repression occurs in the absence of ethylene binding and is achieved through receptor activation of CONSTITUTIVE RESPONSE1 (CTR1), a serine/threonine protein kinase that has sequence similarity to the Raf protein kinase family . CTR1 kinase activity negatively regulates the pathway (i.e., prevents downstream signaling)  (Fig. 3). When ethylene binds to the receptors, ethylene receptor signaling ceases. Consequently, CTR1 is no longer activated and downstream ethylene signaling can proceed (Fig. 3). This model is supported by the fact that null mutations in multiple ethylene receptor genes display constitutive ethylene responses similar to ctr1 loss-of-function mutants, whereas dominant, gain-of-function receptor mutations confer ethylene insensitivity .
What about the biochemical mechanism of ethylene receptor signaling?
This is still unresolved. In the canonical two-component system, binding of the ligand either stimulates or inhibits autophosphorylation of a conserved histidine residue followed by transfer of the phosphate to a conserved aspartate in the receiver domain. Curiously, histidine kinase activity does not appear to play a major role in ethylene receptor signaling [16, 17]. Although the ethylene receptors display histidine and/or serine/threonine kinase activity in vitro, neither activity has been definitively associated with ethylene signaling. In addition, despite hints of two-component signaling elements acting downstream of the receptors, there is strong evidence indicating that this is not the primary mode of ethylene signaling. Instead, the ethylene receptors physically associate with and signal to CTR1 . The receptors also show interaction with the phosphorylation substrate of CTR1, ETHYLENE-INSENSITIVE2 (EIN2) .
Although genetic evidence indicates that ethylene binding inhibits receptor signaling, there is no clear answer to the basic question: Does the binding of ethylene stimulate or inhibit biochemical activity in the receptor? There are data to support each possibility. Although it might be counter-intuitive, a formal possibility is that CTR1 activation occurs by a passive (e.g., steric-based) signaling mechanism that is alleviated when ethylene receptor activity is triggered by the binding of ethylene. Complete structural data for the receptors, which is not yet available, will hopefully shed light on this question. It is also unclear why plants have multiple ethylene receptor isoforms. Although there is evidence that individual receptors have distinct roles in controlling specific responses, the underlying mechanisms for sub-functionalization are unknown .
What happens downstream of the receptors in the ethylene signaling pathway?
Ethylene signaling downstream of CTR1 hinges on the phosphorylation status of EIN2, an enigmatic central regulator of the ethylene-signaling pathway . EIN2 is tethered to the ER membrane by its N-terminal domain, which has sequence similarity to the widely conserved NRAMP metal ion transporters, but the biochemical function of this domain and its role in ethylene signaling have yet to be determined. The C-terminal portion (C-END) of EIN2 consists of a novel plant-specific domain that is cytosolic, and expression of this domain alone is sufficient for the activation of ethylene responses [23, 24]. In the absence of ethylene, the CTR1 kinase phosphorylates the EIN2 C-END, thereby preventing the C-END from signaling . When the receptors detect ethylene, CTR1 is inactivated, and consequently the unphosphorylated EIN2 C-END is proteolytically released from the ER-anchored NRAMP domain [24, 25] (Fig. 3). The cleaved C-END then represses the translation of two F-box proteins, EIN3-BINDING F-BOX1 and 2 (EBF1/2), by binding to the 3’ untranslated regions of EBF1/2 mRNA [26, 27]. This repression, which occurs within discrete cytoplasmic domains (known as P-bodies) where mRNA fates are decided, is crucial in ethylene signaling, because in the nucleus, the EBF1/2 proteins control the proteolytic degradation of two master transcription factors, EIN3/EIL1, which are required for essentially all known ethylene responses . In the absence of ethylene, EBF1/2 target EIN3/EIL1 for ubiquitylation and degradation, in an SCFEBF1/EBF2 ubiquitin-ligating complex ; this is yet another example of negative regulation in the pathway. When ethylene is perceived, EIN2 represses translation of EBF1/2, thereby permitting the EIN3/EIL1 transcription factors to quickly accumulate in the nucleus, leading to rapid responses to ethylene. There is also evidence that the cleaved EIN2 C-END must enter the nucleus in order to activate downstream ethylene signaling, but the exact function of the C-END in the nucleus is unknown.
What happens transcriptionally?
EIN3 was shown to initiate a transcriptional cascade that triggers several dynamic waves of gene expression that include feedback loops and the activation of genes known in numerous other hormone signaling pathways . The primary targets of EIN3 include transcription factor genes in the APETELA2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) family, such as ERF1, which regulates further expression in a transcriptional cascade of ethylene signaling [29, 30]. These global changes in gene expression result in a diverse array of cellular, metabolic and physiological responses .
Where is ethylene research headed?
Given that plant growth, development and stress responses require the integration of diverse environmental and endogenous signals, there is a growing focus on ethylene cross-talk with other signals [31, 32]. Multiple points of ethylene cross-talk have been reported with the plant hormones auxin, gibberellins, brassinosteroids, abscisic acid, cytokinins and jasmonic acid. Cross-talk can occur through the regulation of ethylene biosynthesis, and more work is needed to elucidate such pathways. Conversely, ethylene signaling can induce the biosynthesis of other hormones. For instance, in deep water rice, ethylene signaling induces gibberellins, which signal internode elongation, allowing rice plants to escape from complete submergence . Cross-talk can also occur in the ethylene-signaling pathway. For example, EIN3/EIL1 physically associate with transcription factors that are controlled by gibberellins  and jasmonic acid , promoting or inhibiting gene expression coordinately with these hormones. Given that ein2 mutations have been obtained in genetic screens for components of other signaling pathways , EIN2’s role in translational regulation could be a point of signal integration as well.
Continued advances in understanding cross-talk and transcriptional networks will lead to a deeper understanding of ethylene-signaling networks that will someday allow for the modeling of specific plant responses. Applications of such knowledge have tremendous potential for agricultural improvements. Already, our understanding of ethylene biology can provide new strategies for manipulating ethylene responses, particularly by genetic means. Examples include the delay of flower senescence by expressing a dominant mutant etr1-1 gene of Arabidopsis  and the breeding of flood-tolerant, high-yield rice plants by introducing an ethylene-inducible ERF transcription factor gene (SUB1A) . The days of unknowingly manipulating ethylene biology, e.g., when ancient Chinese burned incense to ripen pears and early Egyptians gashed figs to induce their ripening , are far behind us.
The author thanks Dr. Bram Van de Poel and members of the Chang lab for comments on the manuscript. This work was supported in part by NSF grant MCB-1244303 and a UMD Graduate School Research and Scholarship Award to C.C. C.C. is also supported in part by the Maryland Agricultural Experiment Station.
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- Abeles F, Morgan PW, Saltveit Jr ME. Ethylene in plant biology. 2nd ed. San Diego: Academic Press; 1992.Google Scholar
- McManus MT. Annual plant reviews vol. 44: the plant hormone ethylene. Oxford: Wiley-Blackwell; 2012.View ArticleGoogle Scholar
- Bakshi A, Shemansky JM, Chang C, Binder BM. History of research on the plant hormone ethylene. J Plant Growth Reg. 2015. doi:https://doi.org/10.1007/s00344-015-9522-9.Google Scholar
- Kende H. Plant biology and the Nobel prize. Science. 1998;282:627.View ArticlePubMedGoogle Scholar
- de Poel V. Van der Straeten D. 1-Aminocyclopropane-1-carboxylic acid (ACC) in plants: more than just the precursor of ethylene! Front. Plant Sci. 2014;5:1–11.Google Scholar
- Xu J, Zhang S. Ethylene biosynthesis and regulation in plants. In: Wen C-K, editor. Ethylene in plants. Berlin: Springer; 2015. p. 1–25.Google Scholar
- Abeles FB, Heggestad HE. Ethylene: an urban air pollutant. J Air Pollution Control Assoc. 1973;23:517–21.View ArticleGoogle Scholar
- Dillard MM. Ethylene--the new general anesthetic. J Natl Med Assoc. 1930;22:10–1.PubMedPubMed CentralGoogle Scholar
- Spiller HA, Hale JR, De Boer JZ. The Delphic oracle: a multidisciplinary defense of the gaseous vent theory. J Toxicol Clin Toxicol. 2002;40:189–96.View ArticlePubMedGoogle Scholar
- Bleecker AB, Estelle MA, Somerville C, Kende H. Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science. 1988;241:1086–9.View ArticlePubMedGoogle Scholar
- Guzmán P, Ecker JR. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell. 1990;2:513–23.View ArticlePubMedPubMed CentralGoogle Scholar
- Chang C, Kwok SF, Bleecker AB, Meyerowitz EM. Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators. Science. 1993;262:539–44.View ArticlePubMedGoogle Scholar
- Klee HJ. Ethylene signal transduction: Moving beyond Arabidopsis. Plant Physiol. 2004;135:660–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Guillaume RG, Sauter M. Ethylene biosynthesis and signaling in rice. Plant Sci. 2008;175:32–42.View ArticleGoogle Scholar
- Ju C, Van de Poel B, Cooper ED, Thierer JH, Gibbons TR, Delwiche CF, et al. Conservation of ethylene as a plant hormone over 450 million years of evolution. Nat Plants. 2015;1:Article 14004.View ArticlePubMedGoogle Scholar
- Binder BM, Chang C, Schaller GE. Perception of ethylene by plants--ethylene receptors. In: McManus MT, editor. Annual plant reviews vol. 44: The plant hormone ethylene. Oxford: Wiley-Blackwell; 2012. p. 117–45.View ArticleGoogle Scholar
- Shakeel SN, Wang X, Binder BM, Schaller GE. Mechanisms of signal transduction by ethylene: overlapping and non-overlapping signalling roles in a receptor family. AoB plants. 2013;5:plt010.View ArticlePubMedPubMed CentralGoogle Scholar
- Schaller GE, Bleecker AB. Ethylene-binding sites generated in yeast expressing the Arabidopsis ETR1 gene. Science. 1995;270:1809–11.View ArticlePubMedGoogle Scholar
- Rodriguez FI, Esch JJ, Hall AE, Binder BM, Schaller GE, Bleecker AB. A copper cofactor for the ethylene receptor ETR1 from Arabidopsis. Science. 1999;283:996–8.View ArticlePubMedGoogle Scholar
- Hua J, Meyerowitz EM. Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana. Cell. 1998;94:261–71.View ArticlePubMedGoogle Scholar
- Kieber JJ, Rothenberg M, Roman G, Feldmann KA, Ecker JR. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the Raf family of protein kinases. Cell. 1993;72:427–41.View ArticlePubMedGoogle Scholar
- Bisson MMA, Groth G. New insight in ethylene signaling: autokinase activity of ETR1 modulates the interaction of receptors and EIN2. Mol Plant. 2010;3:882–9.View ArticlePubMedGoogle Scholar
- Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR. EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science. 1999;284:2148–52.View ArticlePubMedGoogle Scholar
- Wen X, Zhang C, Ji Y, Zhao Q, He W, An F, et al. Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus. Cell Res. 2012;22:1613–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Ju C, Yoon GM, Shemansky JM, Lin DY, Ying ZI, Chang J, et al. CTR1 phosphorylates EIN2 to control ethylene signaling from the ER membrane to the nucleus. Proc Natl Acad Sci U S A. 2012;109:19486–91.View ArticlePubMedPubMed CentralGoogle Scholar
- Li W, Ma M, Feng Y, Li H, Wang Y, Ma Y, et al. EIN2-directed translational regulation of ethylene signaling in Arabidopsis. Cell. 2015;163:670–83.View ArticlePubMedGoogle Scholar
- Merchante C, Brumos J, Yun J, Hu Q, Spencer KR, Enrıquez P, et al. Gene-specific translation regulation mediated by the hormone-signaling molecule EIN2. Cell. 2015;163:684–97.View ArticlePubMedGoogle Scholar
- An F, Zhao Q, Ji Y, Li W, Jiang Z, Yu X, et al. Ethylene-induced stabilization of ETHYLENE INSENSITIVE3and EIN3-LIKE1 is mediated by proteasomal degradation of EIN3 Binding F-Box 1 and 2 that requires EIN2in Arabidopsis. Plant Cell. 2010;22:2384–401.View ArticlePubMedPubMed CentralGoogle Scholar
- Chang KN, Zhong S, Weirauch MT, Hon G, Pelizzola M, Li H, et al. Temporal transcriptional response to ethylene gas drives growth hormone cross-regulation in Arabidopsis. Elife. 2013;2, e00675.View ArticlePubMedPubMed CentralGoogle Scholar
- Solano R, Stepanova A, Chao QM, Ecker JR. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Genes Dev. 1998;12:3703–14.View ArticlePubMedPubMed CentralGoogle Scholar
- Vandenbussche F, Van der Straeten D. One for all and all for one: Crosstalk of multiple signals controlling the plant phenotype. J Plant Growth Regul. 2007;26:178–87.View ArticleGoogle Scholar
- Zhu Z, Guo H. Interactions of ethylene and other signals. In: Wen C-K, editor. Ethylene in plants. Berlin: Springer; 2015. p. 135–52.Google Scholar
- Bailey-Serres J, Fukao T, Ronald P, Ismail A, Heuer S, Mackill D. Submergence tolerant rice: SUB1’s journey from landrace to modern cultivar. Rice. 2010;3:138–47.View ArticleGoogle Scholar
- An F, Zhang X, Zhu Z, Ji Y, He W, Jiang Z, et al. Coordinated regulation of apical hook development by gibberellins and ethylene in etiolated Arabidopsis seedlings. Cell Res. 2012;22:915–27.View ArticlePubMedPubMed CentralGoogle Scholar
- Song S, Huang H, Gao H, Wang J, Wu D, Liu X, et al. Interaction between MYC2 and ETHYLENEINSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis. Plant Cell. 2014;26:263–79.View ArticlePubMedPubMed CentralGoogle Scholar
- Wilkinson JQ, Lanahan MB, Clark DG, Bleecker AB, Chang C, Meyerowitz EM, et al. A dominant mutant receptor from Arabidopsis confers ethylene insensitivity in heterologous plants. Nat Biotechnol. 1997;15:444–8.View ArticlePubMedGoogle Scholar
- Merchante C, Alonso JM, Stepanova AN. Ethylene signaling: simple ligand, complex regulation. Curr Opin Plant Biol. 2013;16:554–60.View ArticlePubMedGoogle Scholar