Fig. 3

In vivo biotinylation is highly tissue-specific. a Expression of GFP::TEV::Avi from the intestinal elt-2 promoter. b Expression of GFP::TEV::Avi from the seam and hyp7 epidermal cell-specific wrt-2 promoter. In a and b, the tissue and approximate region imaged are indicated in the cartoon worm. c Western blot detection of GFP::TEV::Avi purified with streptavidin beads from lysates obtained from animals expressing GFP::TEV::Avi and BirA in the same tissue (lanes 1 and 3) or in different tissues (lanes 2 and 4). Scale bars are 10 μm