Fig. 5

Zip71B loss of function can be complemented by hZIP5 but not hZIP4. a Phylogenetic tree revealing the relationship among human and Drosophila Zip members. Tree was generated and displayed by using MEGA software version 4. Zip71B shares the highest homology with human ZIP5. b hZIP5 expression can rescue the sensitivity of Zip71B-RNAi larvae on food supplemented with 1 mM Zinc. Data are presented as means ± SEM; n = 6. ** P < 0.01, *** P < 0.001, two-tailed Student’s t test. Genotypes of the flies used are UAS-EGFP/+; Zip71B-RNAi/+; NP1093-GAL4/+ (Zip71B-RNAi fly), UAS-hZIP5/+; Zip71B-RNAi/+; NP1093-GAL4/+ (Zip71B-RNAi, UAS-hZIP5 fly), and UAS-hZIP4/+; Zip71B-RNAi/+; NP1093-GAL4/+ (Zip71B-RNAi, UAS-hZIP4 fly)