Ion- and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel
- Xiaomin Ou†1,
- Jianli Guo†1,
- Longfei Wang1,
- Hanting Yang1, 3,
- Xiuying Liu1, 3,
- Jianyuan Sun2, 3 and
- Zhenfeng Liu1, 3Email authorView ORCID ID profile
© Liu et al. 2017
Received: 1 January 2017
Accepted: 5 April 2017
Published: 22 April 2017
Trimeric intracellular cation (TRIC) channels are crucial for Ca2+ handling in eukaryotes and are involved in K+ uptake in prokaryotes. Recent studies on the representative members of eukaryotic and prokaryotic TRIC channels demonstrated that they form homotrimeric units with the ion-conducting pores contained within each individual monomer.
Here we report detailed insights into the ion- and water-binding sites inside the pore of a TRIC channel from Sulfolobus solfataricus (SsTRIC). Like the mammalian TRIC channels, SsTRIC is permeable to both K+ and Na+ with a slight preference for K+, and is nearly impermeable to Ca2+, Mg2+, or Cl–. In the 2.2-Å resolution K+-bound structure of SsTRIC, ion/water densities have been well resolved inside the pore. At the central region, a filter-like structure is shaped by the kinks on the second and fifth transmembrane helices and two nearby phenylalanine residues. Below the filter, the cytoplasmic vestibule is occluded by a plug-like motif attached to an array of pore-lining charged residues.
The asymmetric filter-like structure at the pore center of SsTRIC might serve as the basis for the channel to bind and select monovalent cations. A Velcro-like plug-pore interacting model has been proposed and suggests a unified framework accounting for the gating mechanisms of prokaryotic and eukaryotic TRIC channels.
The regulated processes of Ca2+ release from the intracellular stores and its uptake from the cytosol are vital for various biological processes including muscle contraction, neurotransmitter release, cell division, and apoptosis [1, 2]. For instance, muscle contraction is initiated by membrane depolarization followed by opening of a ryanodine receptor (RyR) channel to release Ca2+ from the lumen of the sarcoplasmic reticulum (SR) into the cytosol. The process is known as excitation-contraction (E-C) coupling . Rapid efflux of Ca2+ from the SR generates a transient negative potential inside the SR lumen and will hinder Ca2+ release if the transmembrane potential remains unbalanced. Thus, efficient operation of E-C coupling requires not only the RyR to release Ca2+, but also counteracting ion channels to restore the balance of the SR membrane potential and maintain ion homeostasis within the SR lumen .
Two isoforms of SR/endoplasmic reticulum (ER) membrane proteins, called trimeric intracellular cation (TRIC) channels (TRIC-A and TRIC-B), presumably function as the counteracting ion channels facilitating the intracellular Ca2+ handling processes . Alternatively, they might serve to restore the balance of trans-SR K+ after the RyRs close, instead of carrying countercurrent during Ca2+ release . TRIC channels are permeable to K+ and Na+ with moderate selectivity for K+ over Na+ and are impermeable to Ca2+, Mg2+, or anions [5, 7]. TRIC-A and TRIC-B have distinct single-channel conductances as well as diverse regulatory mechanisms and physiological roles [8, 9]. TRIC-A is regulated by transmembrane voltage  and may interact with the RyR functionally and physically [9, 10]. TRIC-B channel is activated by micromolar Ca2+ applied on the cytosolic side, but it is inhibited by Ca2+ on the luminal side  and is also regulated by voltage . TRIC-B may modulate the Ca2+-release channel activity of inositol trisphosphate (IP3) receptor  and is essential for perinatal lung maturation . Genetic mutations of the human TMEM38B gene (encoding the TRIC-B protein) are found in patients with a hereditary brittle bone disease called osteogenesis imperfecta [13–16]. TMEM38B knockout mice are deficient in producing collagen, and bone mineralization is impaired in the mutant animals [17, 18]. Moreover, TRIC-A contributes to the maintenance of normal blood pressure and may serve as a potential pharmaceutical target for treating hypertension [19, 20]. The association of TRIC channels with bone, pulmonary, and muscular diseases indicates that they have indispensable functions in the related physiological and developmental processes [9, 17, 18, 21].
The structures of TRIC-B channels from Caenorhabditis elegans (CeTRIC-B1 and CeTRIC-B2) revealed a homotrimeric membrane protein-lipid complex with hourglass-shaped pores traversing through each individual monomer . A phosphatidylinositol 4,5-biphosphate (PtdIns(4,5)P2, also known as PIP2) lipid molecule binds specifically to each monomer at 1:1 stoichiometry, mediates trimerization of CeTRIC-B channels, and stabilizes the cytoplasmic gate of the channel. In addition to the members found in eukaryotic organisms, TRIC channel orthologs are widespread in prokaryotes including bacteria and archaea . The prokaryotic TRIC members form the largest group of the TRIC family, outnumbering eukaryotic ones. A recent study suggested that they may mediate K+ uptake, and the structures of two prokaryotic TRIC orthologs from Rhodobacter sphaeroides (RsTRIC) and Sulfolobus solfataricus (SsTRIC) were reported at 3.4- and 2.6-Å resolution, respectively . These prokaryotic orthologs form homotrimeric units resembling those of CeTRIC-B channels, and the ion-conducting pore is also contained within each individual monomer .
Although significant progress has been made on structural and functional studies of both eukaryotic and prokaryotic TRIC channels, the fundamental mechanistic questions regarding the molecular basis of ion selectivity remain largely open. The pore architectures of TRIC channels clearly do not resemble those of homotetrameric K+ channels, such as the well-studied KcsA channel . We do not know where the ion selectivity filter is located in TRIC channels or how TRIC channels selectively bind monovalent cations in their pore region. Therefore, it is indispensable to characterize the ion-binding sites along the permeation pathway of TRIC channels through high-resolution structural studies of TRIC channels. Moreover, the lack of the PIP2 molecule in prokaryotic TRIC orthologs raises questions about how their gates are stabilized in the absence of PIP2 in their structure. Here we describe the detailed ion- and water-binding sites inside the pore of each SsTRIC monomer, define the chemical basis of K+ coordination inside a buried asymmetric filter-like structure, and provide new insights into a Velcro-like plug-pore interacting model accounting for the gating mechanism of TRIC channels.
Ion- and water-binding sites within an hourglass-shaped pore
The tilt angles of transmembrane helices in SsTRIC and CeTRIC-B1/B2
η angle (°)
Both eukaryotic and prokaryotic TRIC channels harbor their ion-conducting pores within each subunit of the homotrimeric assemblies [22, 24]. As shown in Fig. 3c, an hourglass-shaped pore runs through each SsTRIC monomer from cytoplasm to extracellular space. Along the pore, four bottleneck sites, including two (G1 and G2) on the cytoplasmic side, one at the central region (G3), and one close to the extracellular vestibule (G4), form highly constricted areas with extremely small widths at 0.7–2.0 Å (smaller than the diameter of a K+ ion at ~2.8 Å ). For the pore in RsTRIC, the G4 site around Phe103 appears to be slightly wider than the one in SsTRIC, although their overall profiles and pore-lining residues are very similar (Additional file 3: Figure S3a and b). For comparison, the pore within each CeTRIC-B monomer harbors two bottlenecks (instead of four), one on the cytoplasmic side and the other on the luminal side of the ER/SR  (Additional file 3: Figure S3c). The pore lumen surface of SsTRIC is mainly shaped by amino acid residues from M1, M2, M4, and M5 helices. Within the pore, a single file of eight well-resolved spherical densities was observed in the high-resolution electron density map of the K+-soaked crystal (Additional file 4: Figure S4a and b). The four ion/water molecules (P0–P3) on the cytoplasmic side are related to the other four at the P0′–P3′ sites on the extracellular side, and they approximately follow the internal pseudo-C2 symmetry [22, 24] between the M1-M2-M3 and M4-M5-M6 helices. Among these densities, two (P0 and P0′) are located at the pore center area between the G3 and G4 bottleneck sites (Fig. 3c), and they intercalate in the grooves of glycine-rich kinks around Ala44-Gly46 and Gly130-Gly134 regions on M2 and M5, respectively (Fig. 3e and f). Previously, two spherical F o-F c densities at positions similar to P0 and P0′ were observed in the structure of the SsTRIC-Fab complex and assigned as water molecules .
To test whether any of these potential ion-/water-binding sites inside the pore can be accessed by monovalent cations, we have soaked the SsTRIC crystal with Tl+, the electron-rich surrogate of the K+ ion . The anomalous difference Fourier peaks of Tl+ ions are mostly detected on the P0′ and P3′ (outer) sites (Tl-1 and Tl-2 shown in Additional file 4: Figure S4c) inside the pore (and on numerous surface sites), but not on the P0–P3 (inner) sites. By referring to the positions of the Tl+ peaks, the putative K+ binding sites have been assigned in the structure of SsTRIC soaked with KCl. As shown in Fig. 3e and f, the K+ ion bound on the P0′ site is mainly coordinated by the backbone carbonyl from Thr41, backbone amide from Gly45, and a water molecule nearby on the P0 site. Such a coordination mode resembles one half of the K+ coordination observed previously in valinomycin (a K+-permeable ionophore, see Additional file 5: Figure S5a and b), but differs from the square antiprism-type coordination in KcsA (Additional file 5: Figure S5a and c). Due to steric hindrance of the bulky side chain of Phe104, three additional ligands below K+ (as in valinomycin) are absent in SsTRIC. The water molecule on the P0 site is further ligated to the backbone carbonyl groups of Val129 and Gly130 and the backbone amide of Gly133. The coordination bond lengths between K+ and its ligands on P0′ site are consistent with those (2.7–2.9 Å) observed in the KcsA channels, although the coordination geometry of K+ in SsTRIC is of trigonal shape instead of the square antiprism found in KcsA . The introduction of a G45A/G47A or G132A/G133A mutation in RsTRIC (corresponding to A44/G46 and G133/134 in SsTRIC) abolishes its channel activity , indicating that the kinks on M2 and M5 helices are essential for the function of TRIC channels. In these two mutants, the kinks may have been distorted, leading to loss of the P0′ or P0 site (both crucial for K+ binding). Similar kinks are also present on the M2 and M5 helices of CeTRIC-B channels, and a Rb+ ion was found on a site nearby these regions . Thus, such a double-kink structure with an inverted twofold pseudo-symmetry may represent a general characteristic feature of the TRIC channel family.
In SsTRIC, the aromatic rings of Phe16 from the M1 helix and Phe104 from the M4 helix enclose the P0 and P0′ sites from the other side opposing to the M5 kink and M2 kink, respectively (Fig. 3e and f). The side chains of Phe104 and Phe16 not only serve to constrict the pore center to form two bottlenecks (G3 and G4 in Fig. 3c) but may also contribute to binding K+ through cation-π interactions . Mutation of Phe104 to alanine decreases the P K/P Na ratio from 1.21 to 1.08 (as the reversal potential shifts from 4.84 to 1.92 mV under KCl/NaCl bi-ionic conditions, see Fig. 2h and i), and the F104A mutant is also nearly impermeable to Ca2+ or Cl– as the wild type (Fig. 2d and e). Therefore, Phe104 may have a crucial role in adjusting the permeability of SsTRIC to K+ versus Na+, presumably by shaping the geometry of the P0′ site slightly more favorably for binding K+ than Na+. Corresponding to the two phenylalanine residues in SsTRIC, CeTRICB1/B2 channels have their His34/35 and Lys136/Lys137 positioned nearby the kinks to enclose their central ion-binding sites . Such a putative asymmetric cation-binding site at the pore center likely serves as the basis for TRIC channels to selectively bind and conduct monovalent cations (K+ and Na+) when the flanking bottleneck sites become wider at the open state. Besides the K+/Tl+ ion on the P0′ site, the one on the P3′ site in the extracellular vestibule of the pore is coordinated by the side chain carboxyl of Asp50 and the backbone carbonyl of Gly46. This peripheral site is close to the extracellular surface, and it may serve to attract K+ ions before they reach the pore center, or facilitate the release of K+ to the extracellular space.
To further verify whether the ion permeation pathway of the SsTRIC channel does traverse through each monomer as observed in the structure, we have introduced a series of alanine mutations to the key amino acid residues lining the pore lumen surface (Additional file 6: Figure S6a). Expression of the wild-type SsTRIC channel in Escherichia coli induces moderate inhibition of cell growth, while the empty vector or expression of a different membrane protein (the large-conductance mechanosensitive channel from E. coli, EcMscL) has little effect on cell growth (Additional file 6: Figure S6b and c). Such an inhibitory effect is likely due to the basal leaky activity of the SsTRIC channel stimulated by the resting membrane potential (Δψ) of E. coli cells. The activated SsTRIC channel may leak out intracellular K+ ions, perturb the resting potential balance, and inhibit the growth of E. coli cells moderately (Additional file 6: Figure S6b). Mutation of the key residues along the pore lumen surface (such as F104A, D97A, R137A, D138A, M146A, and Y153A) leads to gain-of-function (GOF) phenotypes compared to the wild-type channel (Additional file 6: Figure S6b), resembling the phenotype of a well-established severe GOF mutant (G26H) of MscL [29, 30]. Unlike the other mutants, the F16A protein is undetectable in the membrane fraction (Additional file 6: Figure S6d) or at the whole-cell level, indicating that the protein may have been degraded before forming a functional channel on the membrane due to its high toxicity. As a result, the cells hosting the F16A mutant (not expressing) grow nearly as normal as the empty vector. The control, the R187A mutant (at a site distant from the pore region), expresses normally and shows phenotypes similar to the wild type (Additional file 6: Figure S6c). Hence, the in vivo functional assay data are consistent with the observation of a potential ion permeation pathway contained within each SsTRIC monomer.
Neutral lipid instead of PIP2 bound to SsTRIC
At the monomer-monomer interfaces of the SsTRIC trimer, lipid-like moieties have been observed (Fig. 3a and b), but the identities of these interfacial cofactors are elusive . They have elongated tubular features extending from the peripheral region toward the center of the SsTRIC trimer, and three fatty acyl group-like chains join at the center to form a triskelion-shaped structure enhancing the association of three SsTRIC monomers through hydrophobic interactions. Thin layer chromatography and mass spectrometry analyses of the lipid samples extracted from purified SsTRIC preparations suggest that they belong to highly hydrophobic neutral lipid species (likely a mixture of triacylglycerol and free fatty acids/derivatives from the E. coli membrane, Additional file 7: Figure S7a–c). A similar pattern of lipid bands is also observed in the sample extracted from the pure preparation of TRIC ortholog from E. coli (EcTRIC, Additional file 7: Figure S7a). These lipid molecules are not random species bound to SsTRIC. Instead, they are most likely specific lipids selected by the hydrophobic binding site with a well-defined triskelion shape. Electrospray ionization mass spectrometry analysis detected the presence of a molecule with an m/z value of 987.7, likely corresponding to an ionized triacylglycerol molecule, in the lipid samples extracted from purified SsTRIC protein preparation (Additional file 7: Figure S7b and c). Therefore, the triskelion-shaped density in the SsTRIC trimer is interpreted as a triacylglycerol molecule, and the model fits reasonably well with the electron density (Additional file 7: Figure S7d and e). The amino acid residues involved in binding the lipid are mainly hydrophobic residues from the M2 and M5 helices (Additional file 7: Figure S7e and f). In comparison, CeTRIC-B channels contain a PIP2 lipid molecule per monomer, and the two fatty acyl chains of the PIP2 molecule extend to the monomer-monomer interfaces (Additional file 7: Figure S7g and h), both contributing to trimerization simultaneously . Like the lipid in SsTRIC, the fatty acyl chains of PIP2 molecules in CeTRIC-B channels are also surrounded by hydrophobic residues from the M2 and M5 helices (Additional file 7: Figure S7h and i). Nevertheless, the bulky hydrophilic inositol 4,5-biphosphate head group (covalently linked to the 3-position of the glycerol backbone) of PIP2 inserts through the gap between the M5 and M6 helices, and is wrapped at the center of each monomer to stabilize the cytoplasmic gate of CeTRIC-B channels (Additional file 7: Figure S7g and h). For the lipid molecule in SsTRIC, the three fatty acyl groups (instead of two) extend laterally through the fenestration between M6 and M2′ (from adjacent monomer) helices. Instead of bending toward the pore region within each monomer (like the PIP2 head group), they have reached the external surface facing lipid bilayer (Additional file 7: Figure S7d and e). The two positively charged residues (Lys130 and Arg134 in CeTRIC-B2 shown in Additional file 7: Figure S7h) involved in binding the PIP2 head group in CeTRIC-B are absent in SsTRIC or other prokaryotic orthologs. No bulky hydrophilic head groups are attached to the fatty acyl chains of the lipid found in the SsTRIC channel, suggesting that prokaryotic TRIC channels may adopt a different mechanism to stabilize the cytoplasmic gate. It is noteworthy that Sulfolobus solfataricus and other archaea contain ether-linked lipids (such as sn-2,3-diphytanylglycerol diether and glycerol-dialkyl-calditol-tetraether)  instead of the ester-linked lipids commonly found in bacterial or eukaryotic cells. Therefore, the SsTRIC from its native cellular environments may adopt the ether lipids in its structure.
A tethered plug-like motif at the cytoplasmic gate
On the cytoplasmic side, the region between the M5 and M6 helices forms a loop-helix-loop motif (residues Asn142-Tyr153) (Fig. 4b). It plugs in the inner vestibule of the hourglass-shaped pore and contributes to the formation of the first two bottlenecks (G1 and G2 gates shown in Fig. 3c) within the cytoplasmic vestibule. This plug-like motif binds to Asp97, Arg137, and Asp138 residues lining the surface of the pore lumen, through hydrogen bonds and ionic interactions (Fig. 4c). Thereby, it is stabilized in a position to block the access of ions into the pore (Fig. 4a). Removal of the plug-like motif from the structure generates a wide-open cavity in the cytoplasmic vestibule region (Fig. 4d). In this model, the open cytoplasmic vestibule becomes closer to the pore center and approaches the bottom of the extracellular vestibule.
As shown in Additional file 5: Figure S5d and e, the site for TRIC channels to select and bind monovalent cations (K+/Na+) during their permeation through the pore is most likely located at the central region around the two kinks on the M2 and M5 helices according to the following evidences. Firstly, two discrete water-/ion-binding sites (P0 and P0′) have been located in this region, and one of them (P0′) is accessible to a K+ surrogate (Tl+). Secondly, the coordination bond lengths between the ion on P0′ and the surrounding ligands are consistent with the K+-ligand bond length values observed in the KcsA or valinomycin structures (Additional file 5: Figure S5a–c). Thirdly, the kinks on M2 and M5 are general features conserved in the structures of both prokaryotic and eukaryotic TRIC channels. Such an asymmetric filter-like structure is evidently different from those of KcsA (or other homeo-tetrameric K+ channels) and may represent a new type of monovalent cation-binding site for ion channels. In this filter, K+ was bound in a monohydrated state and forms a trigonal geometry with its ligands, resembling half of the trigonal-antiprism K+ coordinations in valinomycin (Additional file 5: Figure S5a and b). For comparison, the filter in KcsA contains four consecutive layers of discrete K+-binding sites with a square antiprism geometry, and the K+ ion is bound in the center (Additional file 5: Figure S5f). Such a multilayer symmetrical filter in KcsA is more selective (for K+) than the single-layer asymmetric filter in the TRIC channel. The P K/P Na permeability ratio for the KcsA channel is 166.7 or higher , whereas the P K/P Na ratio of the SsTRIC and mammalian TRIC channels is much lower at 1.21 and 1.5 , respectively. Lastly, the F104A mutant of SsTRIC has a lower P K/P Na ratio compared to the wild type, indicating that the mutant becomes less selective between K+ and Na+. The result suggests that the geometry of the fourth pore constriction site (G4) shaped by the bulky side chain of Phe104 and M2/M5 kinks is likely crucial for tuning the relative selectivity of SsTRIC channel for K+ versus Na+.
The plug regions in prokaryotic TRIC orthologs contain a consensus “PX5-7E(D/Q)XYA” motif, while the regions interacting with the plug comprise two consensus motifs, namely the “DA(T/S)XGL” motif on M4 and the “GGXXRD” motif on M5 (the bold residues are those involved in binding the plug). Such conservation suggests that the Velcro-like plug-pore interactions might serve as a general gating mechanism for the prokaryotic TRIC orthologs. These three consensus motifs have become diversified in the eukaryotic members. The comparison between SsTRIC and a CeTRIC-B channel reveals an unexpected diverse feature of the pore plugs among the prokaryotic and eukaryotic members of the family (Additional file 9: Figure S9a and b). While the prokaryotic orthologs utilize a built-in plug in the M5–M6 loop region to control their cytoplasmic gates, the eukaryotic members have evolved a different strategy by adopting a lipid molecule, namely phosphatidylinositol 4,5-bisphosphate (PIP2), as the plug to their pores. The head group of PIP2 latches onto two basic residues on the pore lumen surface and occludes the pore from the cytoplasmic side (Additional file 9: Figure S9b). The M5–M6 loop region in the eukaryotic TRIC proteins turns into an irregular structure capping over the PIP2 head group, instead of forming a plug motif itself. In spite of these evident differences between prokaryotic and eukaryotic members of TRIC family, they share a common overall channel architecture and are probably unified by a general gating mechanism involving a Velcro-like plug-pore interaction. A model accounting for the potential gating mechanism of SsTRIC has been summarized in Additional file 10: Figure S10.
The previous bioinformatics study suggested potential roles of prokaryotic TRIC orthologs in the efflux of metabolites . This prediction awaits verification by further experimental evidences to demonstrate the physiological relevance of TRIC channel function in the transport processes of metabolites. A recent work on RsTRIC and SsTRIC indicated that they are involved in K+ uptake in E. coli cells . Expression of SsTRIC on the E. coli membrane leads to moderate inhibition of cell growth, resembling the phenotype of a gain-of-function ion channel (Additional file 6: Figure S6b). It is likely constitutively active on the E. coli membrane when a resting potential at −220 to −140 mV is present at different growth phases . Putatively, the prokaryotic TRIC members might serve as membrane-potential regulators to prevent imbalance of resting potential (by providing K+ flux) and to maintain ion homeostasis in the cytoplasm of prokaryotic cells, instead of serving as a metabolite transporters themselves. Such a role may serve to regulate the active transport processes of metabolites across the membrane indirectly, as many transporters are driven by the electrochemical potential across the membrane .
In summary, the high-resolution view of ion- and water-binding sites in the SsTRIC channel unravels the presence of an asymmetric filter-like structure buried in the middle of an hourglass pore. The Velcro-like plug-pore interacting model derived in this study may offer a unified framework for understanding the gating mechanism of both prokaryotic and eukaryotic TRIC channels.
Cloning, protein expression, and purification
The gene encoding SsTRIC was synthesized (GenScript) with optimized codon usage for protein expression in Escherichia coli and inserted between the NdeI and XhoI sites in the pET21b vector. The C41 (DE3) E. coli strain was used for protein expression. For large-scale expression, the overnight starter culture was inoculated into Terrific Broth (TB) media at a 1:40 (v:v) ratio. The cells were grown to optical density OD600 = 1.0 and then induced with 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) at 37 °C for 2 h before being harvested through centrifugation.
For the purification of SsTRIC protein, 10 g of defrosted cells were suspended in 100 ml of lysis buffer (50 mM Tris-HCl pH 8.0, 200 mM NaCl, 10 mM imidazole). After homogenization, 1.5 g of dodecyl-β-d-maltoside (DDM) was added to the suspension to extract the membrane proteins, and the mixture was stirred on ice for 30 min. The preparation was sonicated for 2 min (1 s on and 5 s off) in an ice-water bath. The cell lysate was centrifuged for 30 min at 16,000 RPM, 4 °C in a JA-25.50 rotor (Beckman). The supernatant was collected and applied by gravity to a column with 2 ml Ni-NTA superflow resin (Qiagen). After all the sample had flowed through, the column was washed with 10 ml of equilibration buffer (25 mM Tris-HCl pH 7.5, 200 mM NaCl, 20 mM imidazole, 0.5% DM) and then 10 ml of washing buffer (25 mM Tris-HCl pH 7.5, 200 mM NaCl, 80 mM imidazole, 0.4% DM). After the elution buffer (25 mM Tris-HCl pH 7.5, 200 mM NaCl, 300 mM imidazole, 0.4% DM) was applied, the fractions with protein concentration above 0.3 mg ml–1 were pooled and concentrated to 10–15 mg ml–1 in a 50 kDa cutoff Amicon Ultra-4 centrifugal filter unit (Millipore). Next, trypsin was added to the concentrated SsTRIC protein sample at a 1:1000 (m:m) ratio and incubated at 37 °C for 1 h for limited proteolysis treatment. The product was then applied to a Superdex 200 10/300 GL column (GE Healthcare) and eluted in a buffer containing 10 mM Tris HCl pH 7.5, 150 mM NaCl, and 1.1% OG. The major peak fraction of the eluted protein was pooled and concentrated to ~10 mg ml–1 for the crystallization experiments.
Crystallization, crystal soaking, data collection, and processing
The initial crystallization condition was identified through sparse matrix screening using the MemGold kit (Molecular Dimensions). The optimized recipe for crystallization of SsTRIC in OG involves setting up hanging drops with 1 μl 10–15 mg ml–1 protein sample, 1 μl well solution (22–26% PEG 3000, 0.1 M Tris-HCl pH 8.0, 0.2 M sodium acetate, and 0.2 M KCl), and 0.2 μl 30% ethylene glycol. The drops were equilibrated against 0.5 ml of well solution at 16 °C. The best crystals were small hexagonal plates (0.1–0.3 mm in diagonal length) grown on the surface of the drops. To prepare crystal samples with only K+, the plate crystals initially grown in the mixture of NaAc and KCl were washed extensively in a stabilizing solution with KCl as the only salt (26% PEG 3000, 50 mM Tris-HCl pH 8.0, 1.1% OG, 0.1% DM, 3% ethylene glycol, 0.5 M KCl). These crystals were further soaked in the same solution at 16 °C for ~24 h before being harvested. The Tl+-containing crystals were prepared by washing in a stabilizing solution with 0.5 M KNO3 to remove Cl– ions. Otherwise, they would have formed a precipitate with Tl+ ions because TlCl has low solubility in water. Next, the crystals were washed and soaked in a new stabilizing solution with 0.5 M TlNO3 for 24 h.
Data collection, phasing, and structure refinement statistics of SsTRIC
CH3HgCl (A15C mutant)
KCl (wild type)
Cell dimensions (Å, °)
a = 152.07
a = 152.07
a = 150.40
b = 87.48
b = 87.73
b = 86.44
c = 173.62
c = 173.06
c = 173.30
β = 108.75
β = 108.90
β = 108.59
R pim b
No. of heavy atom sites
Figure of merit (before/after DM)
No. of reflections (no. of reflections in free set)
R work (%)
R free (%)
No. atoms (B-factors, Å2)
9054 (37.0) (6 chains)
33 K+ (77.9)
Ramachandran plot (%)c
RMSD bond length (Å)
RMSD bond angles (°)
Structure determination, refinement, and analysis
The structure was solved by the single-wavelength anomalous dispersion method using the Autosol program in the PHENIX suite . The anomalous diffraction dataset of Hg-labeled A15C crystal collected at 1.00000 Å wavelength was used for phasing. Six Hg atoms were located in one asymmetric unit, and the initial set of selected area diffraction (SAD) phases has a figure of merit (FOM) of 0.258 which is further improved to 0.640 through density modification. The initial model automatically generated by the Autobuild program in PHENIX contains a nearly complete polypeptide structural model with R work = 0.250 and R free = 0.260. This model was further improved by iterative cycles of manual adjustment in Coot  and refinement in CNS 1.2  using the high-resolution (2.2 Å) KCl-only data. The longest polypeptide chains were continuously traced from Met 1 to Pro 198. From the electron density map, it appears that the limited proteolysis by trypsin removed only seven residues at the carboxy-terminal region after Pro198 and the hexa-histidine tag region. The transmembrane domain and the loop region remain intact after proteolysis. The K+ ions were modeled and cross-validated by the anomalous difference Fourier peaks of the Tl+ ion. The anomalous difference Fourier map of Tl+-containing crystals was computed by the FFT program in the CCP4 suite . The data collection, phasing, and structure statistics are summarized in Table 2.
For the structural analysis, the PROMOTIF program  was used to analyze the secondary structures, Lsqman  was used to superpose different structures, HOLE  was used to probe the pores within the channel, PISA  was used to analyze protein interfaces and buried surfaces within the trimer, and the APBS  tool was used to calculate surface electrostatic potential. For sequence alignment, the output from the ClustalW  program was manually checked and readjusted. BOXSHADE 3.21 and ESPript programs  were used to generate sequence alignment figures. The cartoon structural figures were produced in PyMOL  or Chimera , and the electron density maps were displayed using Coot .
Lipid extraction and identification
To extract lipids, purified SsTRIC or E. coli TRIC (EcTRIC) protein samples (200 μl 10 mg ml–1 protein in β-DM) were mixed with 180 μl of solvent with chloroform, methanol, and concentrated HCl solution (1:2:0.02, v/v/v). Subsequently, 60 μl of chloroform and 60 μl of 2 M KCl (sigma) were added to each tube. The mixture was vortexed and then centrifuged for 5 min at 2400 g to separate the organic phase from the aqueous phase. The organic phase was then separated through thin layer chromatography (TLC). At first, 20 ul organic phases extracted from SsTRIC and EcTRIC protein samples were spotted on the TLC silica gel plate (Merck). As reference standards, triacylglycerol (TG, C18:1), diacylglycerol (DG, C16:0), monoacylglycerol (MG, C16:1), palmitic acid, and steric acid samples were applied on the same plate. The solvent used for TLC contains n-hexane, diethyl ether, and acetic acid (70:30:1, v:v:v). After being separated on the TLC plate, the lipid fractions were visualized by spraying the plate with 0.5% iodine-chloroform. To further identify the lipid fraction, mass spectroscopy was performed under the electrospray ionization (ESI) positive and negative scan modes. As a control, the organic phase extraction of the blank elution buffer used for protein purification was also examined through mass spectroscopy under the same modes.
Reconstitution of SsTRIC in small unilamellar vesicles (SUVs)
A lipid mixture contraining 90% 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 10% cholesterol (w/w) in chloroform was dried under vacuum in a CentriVap Concentrator (Labconco) for 4 h. The lipid sample was then suspended at 10 mg ml-1 in a low-salt buffer (1 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.2, 5 mM KCl). SUVs were formed by tip sonication (50 Hz, 1 s on, 1 s off for 1 min) and then presolubilized by 10 mM DM for 30 min at RT. Subsequently, the SsTRIC protein sample was added to the presolubilized SUVs to achieve a protein:lipid ratio of 1:10 (m:m). More DM was added to a final concentration of 17.5 mM, and the resulting mixture was gently agitated for 1 h at room temperature. Detergent was removed by dialysis in the low-salt buffer (1 mM HEPES pH 7.2, 5 mM KCl). The external buffer was changed every 12 h for 3 days. After dialysis, the resulting SUVs were aliquoted, flash-frozen in liquid nitrogen, and stored at −80 °C.
Preparation of giant unilamellar vesicles (GUVs) for electrophysiology
The GUV samples were generated by the electroformation technique using the Nanion Vesicle Prep Pro device (Nanion). Before electroformation, trehalose was added to the preformed SUV solution to a final concentration of 10 mM in order to protect the SsTRIC protein during the partial dehydration process. About 10 μl of SUV solution was applied in small droplets (~0.2 μl/droplet) on the indium tin oxide (ITO)-treated glass slide. The droplets were left to dry in the open air for approximately 30 min at room temperature. Subsequently, 300 μl of 1 M sorbitol solution was carefully added onto the lipid film, and a cassette sandwiching the sample in the middle was assembled. During assembly, we ensured that the ITO layers of the slides were facing and that they touched the sample. For the electroformation process, the protocol was set as 0.1 to 1.0 V at 12 Hz frequency for 3 h. For the next step, the frequency was lowered to 4 Hz and the voltage was raised to 2 V for 30 min to detach the GUVs from the glass slides. The temperature was kept constant at 36 °C throughout the electroformation process. Alternatively, the purified protein can be reconstituted directly on the GUVs (protein:lipid = 1:200–250, w:w; lipid: 95% azolectin + 5% cholesterol, w:w) prepared through a modified sucrose method . In some cases when the GUVs did not attach well to the bottom of the sample chamber, the lipid composition was adjusted by adding DPhPC to the mixture at a cholesterol:DPhPC:azolectin (w:w:w) ratio of 1:5:17 or 1:10:15.
All recordings were performed with the inside-out configuration. The intracellular side of the GUV membrane was exposed to the bath solution, and the extracellular side was exposed to the pipette solution. The bath and pipette solutions contained 210 mM KCl and 10 mM HEPES (pH 7.2). Patch pipettes with resistances of 8–9 MΩ were used, and the patch resistance increased to ~2 GΩ after the pipette sealed tightly with the GUV membrane. For recording the data under bi-ionic conditions, the pipette solution was kept constant with 210 mM KCl and 10 mM HEPES (pH 7.2), while the bath solutions were: 210 mM NaCl and 10 mM HEPES (pH 7.2) for P K/P Na analysis; 210 mM KCl, 75 mM CaCl2 or MgCl2, and 10 mM HEPES (pH 7.2) for the test of Ca2+/Mg2+ and Cl- permeability. Single-channel recordings were made with an EPC-10 amplifier (HEKA, Lambrecht, Germany) under different voltage settings at 50 kHz with a 0.5-kHz filter and a 50-Hz notch filter. All experiments were done at room temperature (21–24 °C).
The Clampfit Version 9.0 (Axon Instruments, Foster City, CA) was used for data analysis, Excel Version 2010 (Microsoft) and OriginPro 8 were used for statistical analysis, and Igor Pro 6.32A (WaveMetrics, USA) was used for graphics. The single-channel conductance was obtained through linear fitting of the current-voltage plots. The statistical data are reported as mean value ± SEM. The Student’s t test was used to assess statistical significance; n represented the number of experiments analyzed.
Cell-based functional assay
The plasmids carrying SsTRIC mutants were transformed into the C41 (DE3) E. coli strain for protein expression and in vivo functional assay. For the measurement of cell growth curves, single colonies of the E. coli transformants were used to inoculate 3 ml of liquid LB media plus 100 μg/ml ampicillin and grown at 37 °C for 2–3 h. The cell densities of the cultures were normalized to OD600 = 0.1 with LB media. Subsequently, 75 μl of diluted cell culture was added into the wells of a 96-well plate and mixed with 75 μl LB media with 1 mM IPTG added. The plate was then sealed with the CyclerSeal film (Platemax), and the growth of cells at 37 °C within each well was monitored continuously in the Thermo Varioskan Flash Plate Reader. The OD600 was taken every 20 min while the plate was shaken at 300 cycles of horizontal shakes per minute (SPM).
The protein expression was analyzed through western blots on the membrane fractions of the E. coli cells harvested after being induced by IPTG. To prepare the membrane fractions, 1 g of defrosted cells was suspended in 5 ml lysis buffer (50 mM Tris-HCl pH 8.0, 200 mM NaCl, 10 mM imidazole). Lysozyme was added to a final concentration of 1 mg ml-1, and the suspension was stirred at 4 °C for 1 h. The preparation was sonicated for 2 min in an ice-water bath with a program setting of 1 s on and 5 s off. The cell lysate was spun at 14,800 RPM, 4 °C for 30 min. The supernatant was collected and centrifuged at 14,800 RPM, 4 °C for another 30 min to remove large insoluble debris. Subsequently, the supernatant (1 ml) was ultracentrifuged at 38,000 RPM (~100,000 × g) in an S140AT rotor (Hitachi) at 4 °C for 2 h. The pellet was resuspended in 1 ml of washing buffer (50 mM Tris-HCl pH 8.0, 200 mM NaCl, 10 mM imidazole) and ultracentrifuged again. The membrane pellets were collected and weighted, followed by resuspension in the washing buffer to a final concentration of 50 mg ml-1. The samples were mixed with 5 × SDS loading buffer and then separated by electrophoresis on 12% SDS-PAGE gel. For western blot analysis, the protein bands on the gel were transferred to a nitrocellulose membrane. After blocking and washing, the blot was probed by HRP-conjugated anti-Histag mAb antibody (GenScript, cat. number: A00612, lot number: 16B001004, RRID: AB_915573) and then developed with the Western Lightning ULTRA substrate (PerkinElmer).
We thank D. C. Rees for manuscript reading and advice, F. Q. Yang, T. X. Cai, N. L. Zhu, Z. S. Xie, L. L. Niu, S. M. Li, and J. J. Han for technical assistance on the mass spectrometry analysis of lipid samples, Y. H. Huang for sharing bacterial genomic DNA samples, P. H. Chen for assistance in analyzing the electrophysiology data and manuscript reading, and staff members at SSRF BL17U, Photon Factory (BL1A, BL5A, NE3A and NW12A, Proposal No: 2014G179), and the Protein Science Core Facility at IBP, Chinese Academy of Sciences (CAS) for technical support during data collection.
This project is financially supported by the Strategic Priority Research Program of CAS (XDB08020302), the Ministry of Science and Technology (2014CB910301), the National Natural Science Foundation of China (31670749) and the “135” project from CAS. ZL is supported by the “National Thousand Young Talents Program” from the Office of Global Experts Recruitment in China.
Availability of data and materials
The coordinates and structure factors of SsTRIC have been deposited in the Protein Data Bank (http://www.rcsb.org/pdb) under accession code [Protein Data Bank:5WTR]. The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
XO conducted the cloning, protein expression, purification, and crosslinking experiments and improved crystal quality through extensive optimization procedures. JG performed the electrophysiological experiments, and XO analyzed the data. HY performed site-directed mutagenesis and crystallized the cysteine mutants used for phasing. LW cloned the initial batch of TRIC homologs, tested protein expression, and performed the first round of crystallization screening and optimization. XO, XL, and ZL collected and processed the X-ray diffraction data. JS is involved in the electrophysiological analyses and discussion. ZL coordinated the project, solved the structure, and performed the structural analysis. The manuscript was written by XO, JG, HY, JS, and ZL. All authors read and approved the final manuscript.
The authors declare they have no competing interests.
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- Berridge MJ, Lipp P, Bootman MD. The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol. 2000;1:11–21.View ArticlePubMedGoogle Scholar
- Clapham DE. Calcium signaling. Cell. 2007;131:1047–58.View ArticlePubMedGoogle Scholar
- Ebashi S. Excitation-contraction coupling and the mechanism of muscle contraction. Annu Rev Physiol. 1991;53:1–17.View ArticlePubMedGoogle Scholar
- Fink RH, Veigel C. Calcium uptake and release modulated by counter-ion conductances in the sarcoplasmic reticulum of skeletal muscle. Acta Physiol Scand. 1996;156:387–96.View ArticlePubMedGoogle Scholar
- Yazawa M, et al. TRIC channels are essential for Ca2+ handling in intracellular stores. Nature. 2007;448:78–82.View ArticlePubMedGoogle Scholar
- Guo T, et al. Sarcoplasmic reticulum K+ (TRIC) channel does not carry essential countercurrent during Ca2+ release. Biophys J. 2013;105:1151–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Venturi E, Sitsapesan R, Yamazaki D, Takeshima H. TRIC channels supporting efficient Ca2+ release from intracellular stores. Pflugers Arch. 2013;465:187–95.View ArticlePubMedGoogle Scholar
- Pitt SJ, et al. Charade of the SR K+-channel: two ion-channels, TRIC-A and TRIC-B, masquerade as a single K+-channel. Biophys J. 2010;99:417–26.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou X, et al. Trimeric intracellular cation channels and sarcoplasmic/endoplasmic reticulum calcium homeostasis. Circ Res. 2014;114:706–16.View ArticlePubMedPubMed CentralGoogle Scholar
- Bleunven C, et al. SRP-27 is a novel component of the supramolecular signalling complex involved in skeletal muscle excitation-contraction coupling. Biochem J. 2008;411:343–9.View ArticlePubMedGoogle Scholar
- Matyjaszkiewicz A, et al. Subconductance gating and voltage sensitivity of sarcoplasmic reticulum K+ channels: a modeling approach. Biophys J. 2015;109:265–76.View ArticlePubMedPubMed CentralGoogle Scholar
- Yamazaki D, et al. Essential role of the TRIC-B channel in Ca2+ handling of alveolar epithelial cells and in perinatal lung maturation. Development. 2009;136:2355–61.View ArticlePubMedPubMed CentralGoogle Scholar
- Shaheen R, et al. Study of autosomal recessive osteogenesis imperfecta in Arabia reveals a novel locus defined by TMEM38B mutation. J Med Genet. 2012;49:630–5.View ArticlePubMedGoogle Scholar
- Volodarsky M, et al. A deletion mutation in TMEM38B associated with autosomal recessive osteogenesis imperfecta. Hum Mutat. 2013;34:582–6.PubMedGoogle Scholar
- Rubinato E, et al. A novel deletion mutation involving TMEM38B in a patient with autosomal recessive osteogenesis imperfecta. Gene. 2014;545:290–2.View ArticlePubMedGoogle Scholar
- Lv F, et al. Two novel mutations in TMEM38B result in rare autosomal recessive osteogenesis imperfecta. J Hum Genet. 2016;61:539–45.View ArticlePubMedGoogle Scholar
- Cabral WA, et al. Absence of the ER cation channel TMEM38B/TRIC-B disrupts intracellular calcium homeostasis and dysregulates collagen synthesis in recessive osteogenesis imperfecta. PLoS Genet. 2016;12:e1006156.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhao C, et al. Mice lacking the intracellular cation channel TRIC-B have compromised collagen production and impaired bone mineralization. Sci Signal. 2016;9:ra49.View ArticlePubMedGoogle Scholar
- Yamazaki D, et al. TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance. Cell Metab. 2011;14:231–41.View ArticlePubMedGoogle Scholar
- Tao S, et al. Facilitated hyperpolarization signaling in vascular smooth muscle-overexpressing TRIC-A channels. J Biol Chem. 2013;288:15581–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Takeshima H, Venturi E, Sitsapesan R. New and notable ion-channels in the sarcoplasmic/endoplasmic reticulum: do they support the process of intracellular Ca2+ release? J Physiol. 2015;593:3241–51.View ArticlePubMedGoogle Scholar
- Yang H, et al. Pore architecture of TRIC channels and insights into their gating mechanism. Nature. 2016;538:537–41.View ArticlePubMedGoogle Scholar
- Silverio AL, Saier Jr MH. Bioinformatic characterization of the trimeric intracellular cation-specific channel protein family. J Membr Biol. 2011;241:77–101.View ArticlePubMedPubMed CentralGoogle Scholar
- Kasuya G, et al. Crystal structures of the TRIC trimeric intracellular cation channel orthologues. Cell Res. 2016;26:1288–301.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhou Y, Morais-Cabral JH, Kaufman A, MacKinnon R. Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution. Nature. 2001;414:43–8.View ArticlePubMedGoogle Scholar
- Shannon RD. Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides. Acta Crystallogr A. 1976;32:751–67.View ArticleGoogle Scholar
- Zhou Y, MacKinnon R. The occupancy of ions in the K+ selectivity filter: charge balance and coupling of ion binding to a protein conformational change underlie high conduction rates. J Mol Biol. 2003;333:965–75.View ArticlePubMedGoogle Scholar
- Ma JC, Dougherty DA. The cation-π interaction. Chem Rev. 1997;97:1303–24.View ArticlePubMedGoogle Scholar
- Iscla I, Levin G, Wray R, Reynolds R, Blount P. Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites. Biophys J. 2004;87:3172–80.View ArticlePubMedPubMed CentralGoogle Scholar
- Li J, et al. Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion. Proc Natl Acad Sci U S A. 2015;112:10726–31.View ArticlePubMedPubMed CentralGoogle Scholar
- Jain S, Caforio A, Driessen AJ. Biosynthesis of archaeal membrane ether lipids. Front Microbiol. 2014;26:641.Google Scholar
- LeMasurier M, Heginbotham L, Miller C. KcsA: it’s a potassium channel. J Gen Physiol. 2001;118:303–14.View ArticlePubMedPubMed CentralGoogle Scholar
- Bot CT, Prodan C. Quantifying the membrane potential during E. coli growth stages. Biophys Chem. 2010;146:133–7.View ArticlePubMedGoogle Scholar
- Lemieux MJ. A perspective on the structural studies of inner membrane electrochemical potential-driven transporters. Biochim Biophys Acta. 2008;1778:1805–13.View ArticlePubMedGoogle Scholar
- Battye TGG, Kontogiannis L, Johnson O, Powell HR, Leslie AGW. iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. Acta Crystallogr D. 2011;67:271–81.View ArticlePubMedPubMed CentralGoogle Scholar
- Otwinowski Z, Minor W. Processing of X-ray diffraction data collected in oscillation mode, methods in enzymology. In: Carter Jr CW, Sweet RM, editors. Macromolecular crystallography, part A, vol. 276. New York: Academic Press; 1997. p. 307–26.View ArticleGoogle Scholar
- Adams PD, et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D. 2010;66:213–21.View ArticlePubMedPubMed CentralGoogle Scholar
- Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. Acta Crystallogr D. 2010;66:486–501.View ArticlePubMedPubMed CentralGoogle Scholar
- Brunger AT, et al. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr D. 1998;54:905–21.View ArticlePubMedGoogle Scholar
- Collaborative Computational Project Number 4. The CCP4 suite: programs for protein crystallography. Acta Crystallogr D. 1994;50:760–3.View ArticleGoogle Scholar
- Hutchinson EG, Thornton JM. PROMOTIF—a program to identify and analyze structural motifs in proteins. Protein Sci. 1996;5:212–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Kleywegt GJ. Use of non-crystallographic symmetry in protein structure refinement. Acta Crystallogr D. 1996;52:842–57.View ArticlePubMedGoogle Scholar
- Smart OS, Neduvelil JG, Wang X, Wallace BA, Sansom MSP. HOLE: a program for the analysis of the pore dimensions of ion channel structural models. J Mol Graph. 1996;14:354–60.View ArticlePubMedGoogle Scholar
- Krissinel E, Henrick K. Inference of macromolecular assemblies from crystalline state. J Mol Biol. 2007;372:774–97.View ArticlePubMedGoogle Scholar
- Baker NA, Sept D, Joseph S, Holst MJ, McCammon JA. Electrostatics of nanosystems: application to microtubules and the ribosome. Proc Natl Acad Sci U S A. 2001;98:10037–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Larkin MA, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23:2947–8.View ArticlePubMedGoogle Scholar
- Gouet P, Courcelle E, Stuart DI, Metoz F. ESPript: analysis of multiple sequence alignments in PostScript. Bioinformatics. 1999;15:305–8.View ArticlePubMedGoogle Scholar
- DeLano, WL. The PyMOL Molecular Graphic System. San Carlos: Delano Scientific. 2002;126.96.36.199.Google Scholar
- Pettersen EF, et al. UCSF Chimera—a visualization system for exploratory research and analysis. J Comput Chem. 2004;25:1605–12.View ArticlePubMedGoogle Scholar
- Battle AR, Petrov E, Pal P, Martinac B. Rapid and improved reconstitution of bacterial mechanosensitive ion channel proteins MscS and MscL into liposomes using a modified sucrose method. FEBS Lett. 2009;583:407–12.View ArticlePubMedGoogle Scholar
- Long SB, Campbell EB, MacKinnon R. Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science. 2005;309:897–903.View ArticlePubMedGoogle Scholar
- Catterall WA. Ion channel voltage sensors: structure, function, and pathophysiology. Neuron. 2010;67:915–28.View ArticlePubMedPubMed CentralGoogle Scholar
- Bezanilla F. How membrane proteins sense voltage. Nat Rev Mol Cell Biol. 2008;9:323–32.View ArticlePubMedGoogle Scholar
- von Heijne G. Membrane protein structure prediction: hydrophobicity analysis and the positive-inside rule. J Mol Biol. 1992;225:487–94.View ArticleGoogle Scholar