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  • Erratum
  • Open Access

Erratum to: Charged residues next to transmembrane regions revisited: “Positive-inside rule” is complemented by the “negative inside depletion/outside enrichment rule”

BMC Biology201715:72

  • Received: 25 July 2017
  • Accepted: 27 July 2017
  • Published:

The original article was published in BMC Biology 2017 15:66


Upon publication of the original article [1], the authors noticed that an error was introduced at proofing stage in the panel labels of Fig. 1. Instead of reading ‘a; b; c; d’, these read ‘a; b; c; c’. This has now been updated in the original article and the correct version is included in this erratum. Inconsistencies in the use of brackets around the panel labels of the figure legends have also been corrected and each panel label is now displayed in brackets. We apologise for any inconvenience caused by these errors.

Please see below the correct Fig. 1:
Fig. 1
Fig. 1

Negatively charged amino acids are amongst the rarest residues in TMHs and ±5 flanking residues. Bar charts of the abundance of each amino acid type in the TMHs with flank lengths of the accompanying ±5 residues from the (a) UniHuman single-pass proteins, (b) ExpAll single-pass proteins, (c) UniHuman multi-pass proteins, and (d) ExpAll multi-pass proteins. Amino acid types on the horizontal axis are listed in descending count. The bars were coloured according to categorisations of hydrophobic, neutral and hydrophilic types according to the free energy of insertion biological scale [36]. Grey represents hydrophilic amino acids that were found to have a positive ΔGapp, and blue represents hydrophobic residues with a negative ΔGapp, purple denotes negative residues and positive residues are coloured in orange. The abundances of key residues are labelled



Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

Bioinformatics Institute, Agency for Science Technology and Research (A*STAR), 30 Biopolis Street #07-01, Matrix, Singapore, 138671, Singapore
School of Chemistry, Manchester Institute of Biotechnology, 131 Princess Street, Manchester, M1 7DN, UK
School of Computer Engineering (SCE), Nanyang Technological University (NTU), 50 Nanyang Drive, Singapore, 637553, Singapore


  1. Baker JA, Wong W;C, Eisenhaber B, Warwicker J and Eisenhaber F: Charged residues next to transmembrane regions revisited: “Positive-inside rule” is complemented by the “negative inside depletion/outside enrichment rule”. BMC Biology 2017, 15:66. DOI: 10.1186/s12915-017-0404-4


© Eisenhaber et al. 2017