The integration of chloroplast protein targeting with plant developmental and stress responses
© Schnell et al. 2017
Published: 7 December 2017
The plastids, including chloroplasts, are a group of interrelated organelles that confer photoautotrophic growth and the unique metabolic capabilities that are characteristic of plant systems. Plastid biogenesis relies on the expression, import, and assembly of thousands of nuclear encoded preproteins. Plastid proteomes undergo rapid remodeling in response to developmental and environmental signals to generate functionally distinct plastid types in specific cells and tissues. In this review, we will highlight the central role of the plastid protein import system in regulating and coordinating the import of functionally related sets of preproteins that are required for plastid-type transitions and maintenance.
Plastid protein import: from cytoplasm to organelle
The plastids are a structurally and functionally diverse group of inter-related organelles that are a hallmark feature of plants and algae. In addition to the reactions of photosynthesis in the chloroplasts of green tissues, the plastids confer a remarkable degree of metabolic diversity in all plant tissues. This includes basic components of amino acid and lipid metabolism, and specialized metabolism, including starch metabolism in roots and tubers by amyloplasts, carotenoid synthesis in ripening fruit and some floral tissues by chromoplasts, and the synthesis of tissue-specific phytohormones and key defense and signaling molecules in a variety of tissues. Plastids are essential organelles that are derived from undifferentiatied proplastids and undergo specific differentiation to fulfill specialized functions, depending on developmental changes or physiological and environmental conditions [1, 2]. Plastid differentiation also is reversible and different plastid types can rapidly interconvert depending on the metabolic demands of the cell or tissue type.
A number of groups, including our own, have shown that the import of the majority of nucleus-encoded proteins into plastids is mediated by two multi-component transport systems at the double membrane envelope, referred to as the TOC (transocon at the outer membrane of chloroplasts) and TIC complexes (transocon at the inner membrane of chloroplasts) (Fig. 1a) [3, 4, 6, 12–14]. The intrinsic transit peptide of the nuclear-encoded plastid preprotein mediates import, and is recognized sequentially by TOC-TIC components and associated chaperones to drive selective, unidirectional transport through membrane channels at both envelope membranes [15–17]. The TOC-TIC system is aided by an additional array of molecular chaperones that facilitate targeting and membrane translocation of preproteins .
Traditionally considered a housekeeping function, it is now clear that protein import is a highly dynamic and regulated system, which adapts to accommodate the dramatic changes in the flux and profiles of imported proteins that occur during developmental and biogenetic events [6, 14]. This review will highlight recent studies that reveal 1) the diversity and regulation of the import apparatus that is required to mediate the import of different coordinately expressed proteins during developmental changes; 2) the concerted interactions of the TOC-TIC system with the chaperone and protein quality control networks in the cytoplasm, intermembrane space, and plastid stroma that maintain organelle proteostasis; and 3) the relationship of the protein import apparatus with sub-organellar targeting pathways that is essential to the organization and remodeling of organelle architecture.
Diverse TOC complexes and the ubiquitin-proteasome system interact to maintain the balance of protein import during plastid biogenesis
The ability of plastids to undergo rapid functional diversification in different cell types requires a tight coordination between nuclear gene expression, translation, protein import, and assembly of organelle proteins . For example, the light-induced transition of non-green plastids to photoautotrophic chloroplasts in green seedlings triggers the expression of hundreds of photosynthetic proteins that must be imported and assembled . As a consequence, the preprotein targeting and import apparatus must dramatically increase its capacity to accommodate new sets of highly expressed proteins while maintaining the delivery and import of constitutively expressed plastid genes that are essential for basic organelle function.
Evidence is accumulating in support of the existence of distinct pathways for the import of specific classes of preproteins, thereby preventing competition for import between proteins from different functional or developmental-specific groups. The distinct pathways also provide a mechanism to selectively regulate the import of different functional groups of plastid preproteins (Fig. 1b). Studies indicate that the import of different preproteins varies between distinct plastid types or developmental stages of plant tissues [19, 20]. One detailed study identified at least three classes of preproteins whose import varies depending on the developmental stage of chloroplasts . These include preproteins that are preferentially imported in newly developing chloroplasts (Group I), in mature chloroplasts (Group III), or those whose import efficiency is independent of the stage of plastid development (Group II). These data suggest that the import apparatus and transit peptides have coordinately evolved to optimize the import of different preproteins at distinct stages of organelle development [16, 17].
The functional diversity of transit peptides is consistent with the existence of functionally distinct TOC complexes and also supports the importance of multiple import pathways in plastid biogenesis [21–26]. Preproteins are recognized at the chloroplast surface by two related GTPase receptor families of the TOC complex, referred to as the Toc159 and Toc34 receptor families (the numbers refer to their predicted molecular sizes) [27–29]. We and others have proposed that Toc159 and Toc34 work coordinately to recognize the transit peptides of preproteins in the cytoplasm and initiate transport through an associated outer membrane channel, Toc75, via a GTP-regulated switch [6, 30–32]. Toc159 and Toc34 are encoded by small, differentially expressed gene families, and it has been demonstrated that the selectivity of TOC complexes for different preproteins in vitro and in vivo is determined by the GTPase receptor component of the TOC complex, primarily the specific Toc159 family member (Fig. 1b) [21, 25, 33]. In the ppi2 mutant of Arabidopsis thaliana TOC159 (atToc159), the gene encoding the dominant Toc159 family member in the green tissues of Arabidopsis, plastids fail to develop into fully functional chloroplasts and the plants exhibit a pronounced albino phenotype . The plants are unable to transition from chemoautotrophic to photoautotrophic growth, indicating that atTOC159 is required for the transition from non-green plastids to chloroplasts. Two other members of the Toc159 family, atToc132 and atToc120, are constitutively expressed in all tissues and appear to have overlapping transit peptide specificities [22, 26] that are distinct from atToc159 . Their absence is lethal at an early stage of plant embryo development, and analysis of the atToc132 receptor suggests that it is required for the import of genes involved in basic plastid metabolism present in all tissues (e.g., amino acid and lipid synthesis) [22, 26]. The expression profiles of atToc159 and atToc120/132 correlate with the Group I and Group II classes of transit peptides, suggesting that they are primary determinants of distinct import pathways that are required for plastid maintenance and differentiation . Detailed analyses of the transcriptome and proteome of ppi2 plants suggest that the atToc159 pathway is involved in the biogenesis of a much broader spectrum of proteins than simply photosynthetic proteins, and that the contribution of distinct TOC complexes to plastid differentiation is complex and involves multiple import pathways . Consistent with this conclusion, the Toc159 family members are expressed in all tissues examined, albeit at different absolute and relative levels [22, 26, 35, 36].
Despite the correlation between functionally differentiated classes of transit peptides and import receptors with distinct specificities, it remains unclear how a small number of import receptors (six identified to date) can accommodate the remarkable diversity of transit peptide sequences . Li and Teng  have proposed a “multi-selection and multi-order” model for transit peptide organization to account for some of this functional diversity. They propose that transit peptides are comprised of sets of motifs that specify interactions with specific translocon components. The assembly of these motifs in a variety of combinations could then determine the plastid-type preferences for import and potentially determine the relative affinities and efficiencies of import by utilizing a fairly small number of import components.
The importance of distinct import pathways is highlighted by the discovery that regulated proteolysis via the cytoplasmic ubiquitin proteasome system (UPS) pathway controls the relative abundance of TOC complexes that are required for plastid-type transitions (Fig. 1b). Mutations in SP1, an integral outer membrane E3 ligase, disrupt the transition from etioplasts to chloroplasts in developing seedlings and chloroplasts to gerontoplasts in senescing plants . Conversely, overexpression of SP1 promotes the etioplast to chloroplast transition and triggers early senescence. SP1 participates in the ubiquitination of both GTPase receptors and Toc75, and proteasome inhibitors inhibit their degradation . Interestingly, SP1 expression and associated TOC turnover were increased under abiotic stress conditions that increased chloroplast ROS production . These observations led to the hypothesis that regulated proteolysis is required for selective turnover of specific TOC complexes, thereby altering the balance of import pathways that are required for plastid-type transitions or in response to cell stress [40–42].
Chaperone and quality control networks monitor preprotein competence during protein import
There is also evidence to suggest that components of the cytosolic chaperone complexes work in conjunction with a cytosolic quality control system to ensure that plastid preproteins do not accumulate in the cytoplasm. An Hsp70 isoform, Hsc70-4, functions in conjunction with the cytosolic E3 ubiquitin ligase, CHIP, to target misfolded or mis-sorted preproteins for degradation by the cytosolic UPS (Fig. 2) [47, 48]. Mutations in TOC components that decrease protein import efficiency result in the accumulation of preproteins in the cytoplasm [26, 36, 49] and induce the expression of Hsc70-4 . Likewise, reduction in Hsc70-4 expression results in the appearance of cytosolic preproteins . Specific recognition of cytosolic preproteins by Hsc70-4 for degradation appears to involve the transit peptide  and is correlated with detectable N-acetylation of cytosolic preproteins , suggesting a possible mechanism for the detection and marking of preproteins for selective degradation. It is not entirely clear how the quality control system distinguishes preproteins that are on a productive import route from those that are accumulating or import incompetent. However, the mechanism likely involves the probability of being detected by the quality control system during the transit time from the completion of protein synthesis to binding of the transit peptide at the TOC complex. Under optimal conditions, preprotein transit time would be short, reducing the probability of recognition by Hsc70-4/CHIP in the cytoplasm.
An additional intermembrane space protein, Tic22, crosslinks with preproteins and directly interacts with chloroplast preproteins in vitro [55, 57]. The Tic22 orthologue from the apicomplexans Plasmodium falciparum and Toxoplasma gondii display chaperone activity in vitro . Therefore, it has been proposed that Tic22 acts as a chaperone in the intermembrane space to facilitate the continuity of precursor translocation between the TOC and TIC channels (Fig. 3a). Plants expressing POTRA-deleted versions of Toc75 showed a dramatic increase in Tic22 levels, and the POTRA domains of Toc75 interact with Tic22 in vitro, suggesting a functional link between these intermembrane space components . The crystal structure of the Toc75 POTRA domains was recently solved and reveals features unique to Toc75 compared to related members of the Omp85 family from bacteria [56, 59, 60]. POTRA2 and 3 (the two β-barrel-adjacent POTRAs) are critical for binding precursors and in vitro chaperone activity , suggesting that together the POTRA domains of Toc75 and Tic22 act as a chaperone system for preproteins as they traverse the intermembrane space and engage the TIC complex.
To gain access to the chloroplast stroma, precursors also must be translocated across the chloroplast inner envelope, requiring the establishment of a link between the TOC and TIC complexes. Strong evidence indicates that Tic20, a multi-spanning membrane protein at the inner membrane, is a key link between TOC and TIC and likely forms the channel in the inner envelope [57, 61–63]. A second protein with similarity to Tic20, Tic21, might also participate at this stage [62, 64]. In Arabidopsis, Tic20 assembles into a “1 MDa complex” containing Tic100 and Tic56, which are both localized to the intermembrane space, and Tic214, a large membrane protein encoded by the chloroplast genome . The 1 MDa complex interacts with preproteins at an intermediate stage in import, suggesting that it might facilitate the handoff of the preprotein from the Toc75-Tic22 complex to the Tic20 channel (Fig. 3a) [61, 65, 66]. Although Tic20 is conserved in all plant genomes examined, Tic214, Tic100, and Tic56 are absent from the grasses, suggesting that TIC architecture has diverged in some plant lineages.
The import-associated chaperone network in the stroma functions as an import motor and quality control system
Although the precise nature of the membrane translocation events at the TIC complex remain to be defined, preproteins have been shown to interact with components of a stromal import-associated chaperone network immediately upon exposure to the stroma (Fig. 3b). In addition to the 1MDa complex containing Tic56, Tic100, and Tic214, numerous groups, including our own, have shown that Tic20 also interacts with a large membrane-bound protein, Tic110 [57, 62, 67–70]. Tic110 has a large C-terminal soluble domain protruding into the stroma that acts as a scaffold for chaperone binding [67–69, 71, 72], and it associates with a co-chaperone protein, Tic40, which is embedded in the inner envelope by a single TMD [73, 74]. Tic110 and Tic40 are responsible for assembling the chaperone network, including chloroplast Hsp70, Hsp90, and Hsp93/ClpC, at the site of import to facilitate membrane translocation, folding or suborganellar targeting of preproteins (Fig. 3b).
A primary function of the import-associated chaperone network is to bind preproteins as they emerge from the TIC channel and provide the motor for translocation across the envelope membranes (Fig. 3b) [18, 75]. The relative contributions of the three chaperones to the import motor or downstream chaperone activities has not been completely resolved, but all three chaperones appear to play essential roles in the later stages of import [76–79]. The transit peptide appears to initially dock at a site near the membrane on Tic110 [67, 80]. This interaction facilitates the association of Tic110 with Hsp93/ClpC, a AAA+-ATPase chaperone family member [81–85], and results in hand-off of the preprotein to the chaperone through the activity of Tic40 . Chloroplast Hsp70 and Hsp90 also associate with this complex via interactions with Tic110 [77, 79]. Hsp70 homologs are well established to function in protein translocation in other organelles (mitochondria and the endoplasmic reticulum), and Arabidopsis knockouts of each of two chloroplast Hsp70 homologs show import defects in vitro . Chloroplast Hsp70 immunoprecipitates with preproteins and other TOC/TIC components during the late stages of import . Interestingly, the expression of chloroplast Hsp70 mutants with altered ATPase activities in the moss Physcomitrella cause corresponding changes in the ATP-dependence of protein translocation across the envelope, providing strong evidence that Hsp70 plays a major role in the translocation motor [79, 86]. Many transit peptides also possess Hsp70-binding motifs that are important for protein import [15, 87], further supporting the central role of both cytosolic and stromal Hsp70s in preprotein targeting and membrane translocation. Hsp90 (Hsp90C) was more recently discovered as an import associated chaperone [25, 88]. We showed that Hsp90C associates with TIC components, and that Hsp90C inhibitors block the later stages of import, consistent with a role as part of the import motor .
The complexity of the plastid import-associated chaperone network raises interesting questions about other potential functions for this complex downstream of import. This includes a possible quality control function to monitor the folding, assembly, or suborganellar targeting of newly imported proteins (Fig. 3b). While the majority of chloroplast Hsp93/ClpC exists in the stroma where it associates with the ClpP/R core as part of the Clp protease complex [89–91], a significant proportion of Hsp93/ClpC is associated with the envelope through an interaction with Tic110 [73, 92, 93]. Evidence suggests that the envelope-associated Hsp93/ClpC is also part of an intact Clp protease complex [18, 93]. These results suggest that envelope-associated Hsp93/ClpC might also function in degradation of preproteins shortly after import, either to regulate their levels within the chloroplast or to serve as an import-associated quality control mechanism (Fig. 3b).
Cooperation between import and suborganellar targeting machineries optimizes suborganellar targeting
The nuclear and plastid genomes also encode proteins destined to the inner envelope membrane [88, 107, 108] and evidence suggests a link between import of a subset of these proteins and their integration into the inner membrane (Fig. 4b). The inner membrane has its own conserved SEC system, designated cpSec2, which appears to mediate the integration of proteins following synthesis in the stroma or after import via TOC-TIC [109, 110]. Our own examination of the post-import/conservative pathway demonstrated that a single-pass inner membrane protein with an N-terminal transmembrane helix could integrate while still in the process of import through TOC-TIC . In addition, Li and colleagues showed that FtsH12, an integral inner membrane protein, is targeted in a coupled import-integration manner, and they proposed that its two transmembrane domains and the intervening loop are inserted into the inner membrane from the stroma side via cpSec2 as the remainder of the polypeptide is imported through TOC-TIC . This model is analogous to the insertion of mitochondrial Mdl1, in which the TIM23 machinery and the Oxa1 translocase cooperate to insert the protein at the inner mitochondrial membrane . It will be interesting to see if this cooperation between the import and insertion pathways is a general feature for the insertion of inner membrane proteins. Regardless, it is clear from studies of targeting to the thylakoid and chloroplast inner envelope that mechanisms have evolved to minimize the probability of aggregation and mis-sorting of hydrophobic membrane proteins as they transit the stroma en route to their final destination.
Protein import as a key control point in development and environmental responses
It is now clear from recent studies that the plastid protein import machinery functions both as an essential organellar protein targeting system and a key control point in remodeling and balancing the plastid proteome during developmental transitions and stress. This opens an exciting new focus on better understanding how the regulation and monitoring of protein import fits into the transcriptional and translational regulatory networks that contribute to organelle biogenesis, developmental transitions, and stress responses in plant systems.
This work was supported by National Institutes of Health Grant 2RO1-GM061893 to DJS.
LGLR, RS, and DJS prepared figures and wrote and revised the article. All authors have read and agreed with the content.
The authors declare that they have no competing interests.
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- Jarvis P, Lopez-Juez E. Biogenesis and homeostasis of chloroplasts and other plastids. Nat Rev Mol Cell Biol. 2013;14(12):787–802.PubMedView ArticleGoogle Scholar
- Kami C, Lorrain S, Hornitschek P, Fankhauser C. Light-regulated plant growth and development. Curr Top Dev Biol. 2010;91:29–66.PubMedView ArticleGoogle Scholar
- Li HM, Chiu CC. Protein transport into chloroplasts. Annu Rev Plant Biol. 2010;61:157–80.PubMedView ArticleGoogle Scholar
- Shi LX, Theg SM. The chloroplast protein import system: from algae to trees. Biochim Biophys Acta. 2013;1833(2):314–31.PubMedView ArticleGoogle Scholar
- Celedon JM, Cline K. Intra-plastid protein trafficking: how plant cells adapted prokaryotic mechanisms to the eukaryotic condition. Biochim Biophys Acta. 2013;1833(2):341–51.PubMedView ArticleGoogle Scholar
- Paila YD, Richardson LG, Schnell DJ. New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development. J Mol Biol. 2015;427(5):1038–60.PubMedView ArticleGoogle Scholar
- Lopez-Juez E. Plastid biogenesis, between light and shadows. J Exp Bot. 2007;58(1):11–26.PubMedView ArticleGoogle Scholar
- Lopez-Juez E, Pyke KA. Plastids unleashed: their development and their integration in plant development. Int J Dev Biol. 2005;49(5-6):557–77.PubMedView ArticleGoogle Scholar
- Kleffmann T, von Zychlinski A, Russenberger D, Hirsch-Hoffmann M, Gehrig P, Gruissem W, et al. Proteome dynamics during plastid differentiation in rice. Plant Physiol. 2007;143(2):912–23.PubMedPubMed CentralView ArticleGoogle Scholar
- Barsan C, Sanchez-Bel P, Rombaldi C, Egea I, Rossignol M, Kuntz M, et al. Characteristics of the tomato chromoplast revealed by proteomic analysis. J Exp Bot. 2010;61(9):2413–31.PubMedView ArticleGoogle Scholar
- Yu HT, Xu SB, Zheng CH, Wang T. Comparative proteomic study reveals the involvement of diurnal cycle in cell division, enlargement, and starch accumulation in developing endosperm of Oryza sativa. J Proteome Res. 2012;11(1):359–71.PubMedView ArticleGoogle Scholar
- Abdallah F, Salamini F, Leister D. A prediction of the size and evolutionary origin of the proteome of chloroplasts of Arabidopsis. Trends Plant Sci. 2000;5(4):141–2.PubMedView ArticleGoogle Scholar
- Leister D. Chloroplast research in the genomic age. Trends Genet. 2003;19(1):47–56.PubMedView ArticleGoogle Scholar
- Sjuts I, Soll J, Bolter B. Import of soluble proteins into chloroplasts and potential regulatory mechanisms. Front Plant Sci. 2017;8:168.PubMedPubMed CentralView ArticleGoogle Scholar
- Chotewutmontri P, Reddick LE, McWilliams DR, Campbell IM, Bruce BD. Differential transit peptide recognition during preprotein binding and translocation into flowering plant plastids. Plant Cell. 2012;24(7):3040–59.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee DW, Woo S, Geem KR, Hwang I. Sequence motifs in transit peptides act as independent functional units and can be transferred to new sequence contexts. Plant Physiol. 2015;169(1):471–84.PubMedPubMed CentralView ArticleGoogle Scholar
- Li HM, Teng YS. Transit peptide design and plastid import regulation. Trends Plant Sci. 2013;18(7):360–6.PubMedView ArticleGoogle Scholar
- Flores-Perez U, Jarvis P. Molecular chaperone involvement in chloroplast protein import. Biochim Biophys Acta. 2013;1833(2):332–40.PubMedView ArticleGoogle Scholar
- Dahlin C, Cline K. Developmental regulation of the plastid protein import apparatus. Plant Cell. 1991;3:1131–40.PubMedPubMed CentralView ArticleGoogle Scholar
- Teng YS, Chan PT, Li HM. Differential age-dependent import regulation by signal peptides. PLoS Biol. 2012;10(10), e1001416.PubMedPubMed CentralView ArticleGoogle Scholar
- Dutta S, Teresinski HJ, Smith MD. A split-ubiquitin yeast two-hybrid screen to examine the substrate specificity of atToc159 and atToc132, two Arabidopsis chloroplast preprotein import receptors. PLoS One. 2014;9(4), e95026.PubMedPubMed CentralView ArticleGoogle Scholar
- Kubis S, Patel R, Combe J, Bedard J, Kovacheva S, Lilley K, et al. Functional specialization amongst the Arabidopsis Toc159 family of chloroplast protein import receptors. Plant Cell. 2004;16(8):2059–77.PubMedPubMed CentralView ArticleGoogle Scholar
- Bauer J, Chen K, Hiltbunner A, Wehrli E, Eugster M, Schnell D, et al. The major protein import receptor of plastids is essential for chloroplast biogenesis. Nature. 2000;403(6766):203–7.PubMedView ArticleGoogle Scholar
- Infanger S, Bischof S, Hiltbrunner A, Agne B, Baginsky S, Kessler F. The chloroplast import receptor Toc90 partially restores the accumulation of Toc159 client proteins in the Arabidopsis thaliana pp i2 mutant. Mol Plant. 2011;4(2):252–63.PubMedView ArticleGoogle Scholar
- Inoue H, Rounds C, Schnell DJ. The molecular basis for distinct pathways for protein import into Arabidopsis chloroplasts. Plant Cell. 2010;22(6):1947–60.PubMedPubMed CentralView ArticleGoogle Scholar
- Ivanova Y, Smith MD, Chen K, Schnell DJ. Members of the Toc159 import receptor family represent distinct pathways for protein targeting to plastids. Mol Biol Cell. 2004;15(7):3379–92.PubMedPubMed CentralView ArticleGoogle Scholar
- Chang WL, Soll J, Bolter B. The gateway to chloroplast: re-defining the function of chloroplast receptor proteins. Biol Chem. 2012;393(11):1263–77.PubMedView ArticleGoogle Scholar
- Kessler F, Schnell D. Chloroplast biogenesis: diversity and regulation of the protein import apparatus. Curr Opin Cell Biol. 2009;21(4):494–500.PubMedView ArticleGoogle Scholar
- Schleiff E, Becker T. Common ground for protein translocation: access control for mitochondria and chloroplasts. Nat Rev Mol Cell Biol. 2011;12(1):48–59.PubMedView ArticleGoogle Scholar
- Agne B, Kessler F. Protein transport in organelles: the Toc complex way of preprotein import. Febs J. 2009;276(5):1156–65.PubMedView ArticleGoogle Scholar
- Kessler F, Schnell DJ. Chloroplast protein import: solve the GTPase riddle for entry. Trends Cell Biol. 2004;14(7):334–8.PubMedView ArticleGoogle Scholar
- Li HM, Kesavulu MM, Su PH, Yeh YH, Hsiao CD. Toc GTPases. J Biomed Sci. 2007;14(4):505–8.PubMedView ArticleGoogle Scholar
- Smith MD, Rounds CM, Wang F, Chen K, Afitlhile M, Schnell DJ. atToc159 is a selective transit peptide receptor for the import of nucleus-encoded chloroplast proteins. J Cell Biol. 2004;165:323–34.PubMedPubMed CentralView ArticleGoogle Scholar
- Bischof S, Baerenfaller K, Wildhaber T, Troesch R, Vidi PA, Roschitzki B, et al. Plastid proteome assembly without Toc159: photosynthetic protein import and accumulation of N-acetylated plastid precursor proteins. Plant Cell. 2011;23(11):3911–28.PubMedPubMed CentralView ArticleGoogle Scholar
- Gutensohn M, Schulz B, Nicolay P, Flügge UL. Functional analysis of two Toc34 homologues in Arabidopsis indicates specialized functions in vivo. Plant J. 2000;23:771–83.PubMedView ArticleGoogle Scholar
- Jarvis P, Chen L-J, Li H, Peto CA, Fankhauser C, Chory J. An Arabidopsis mutant defective in the plastid general protein import apparatus. Science. 1998;282:100–3.PubMedView ArticleGoogle Scholar
- von Heijne G, Nishikawa K. Chloroplast transit peptides: the perfect random coil? FEBS Lett. 1991;278:1–3.View ArticleGoogle Scholar
- Ling Q, Huang W, Baldwin A, Jarvis P. Chloroplast biogenesis is regulated by direct action of the ubiquitin-proteasome system. Science. 2012;338(6107):655–9.PubMedView ArticleGoogle Scholar
- Ling Q, Jarvis P. Regulation of chloroplast protein import by the ubiquitin E3 ligase SP1 is important for stress tolerance in plants. Curr Biol. 2015;25(19):2527–34.PubMedPubMed CentralView ArticleGoogle Scholar
- Broad W, Ling Q, Jarvis P. New insights into roles of ubiquitin modification in regulating plastids and other endosymbiotic organelles. Int Rev Cell Mol Biol. 2016;325:1–33.PubMedView ArticleGoogle Scholar
- Ling Q, Jarvis P. Dynamic regulation of endosymbiotic organelles by ubiquitination. Trends Cell Biol. 2013;23(8):399–408.PubMedView ArticleGoogle Scholar
- Ling Q, Jarvis P. Functions of plastid protein import and the ubiquitin-proteasome system in plastid development. Biochim Biophys Acta. 2015;1847(9):939–48.PubMedView ArticleGoogle Scholar
- Fellerer C, Schweiger R, Schongruber K, Soll J, Schwenkert S. Cytosolic HSP90 cochaperones HOP and FKBP interact with freshly synthesized chloroplast preproteins of Arabidopsis. Mol Plant. 2011;4(6):1133–45.PubMedView ArticleGoogle Scholar
- Qbadou S, Becker T, Mirus O, Tews I, Soll J, Schleiff E. The molecular chaperone Hsp90 delivers precursor proteins to the chloroplast import receptor Toc64. EMBO J. 2006;25(9):1836–47.PubMedPubMed CentralView ArticleGoogle Scholar
- Martin T, Sharma R, Sippel C, Waegemann K, Soll J, Vothknecht UC. A protein kinase family in Arabidopsis phosphorylates chloroplast precursor proteins. J Biol Chem. 2006;281(52):40216–23.PubMedView ArticleGoogle Scholar
- May T, Soll J. 14-3-3 proteins form a guidance complex with chloroplast precursor proteins in plants. Plant Cell. 2000;12(1):53–64.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee S, Lee DW, Lee Y, Mayer U, Stierhof YD, Lee S, et al. Heat shock protein cognate 70-4 and an E3 ubiquitin ligase, CHIP, mediate plastid-destined precursor degradation through the ubiquitin-26S proteasome system in Arabidopsis. Plant Cell. 2009;21(12):3984–4001.PubMedPubMed CentralView ArticleGoogle Scholar
- Shen G, Adam Z, Zhang H. The E3 ligase AtCHIP ubiquitylates FtsH1, a component of the chloroplast FtsH protease, and affects protein degradation in chloroplasts. Plant J. 2007;52(2):309–21.PubMedView ArticleGoogle Scholar
- Agne B, Infanger S, Wang F, Hofstetter V, Rahim G, Martin M, et al. A toc159 import receptor mutant, defective in hydrolysis of GTP, supports preprotein import into chloroplasts. J Biol Chem. 2009;284(13):8670–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Inoue H, Akita M. Three sets of translocation intermediates are formed during the early stage of protein import into chloroplasts. J Biol Chem. 2008;283(12):7491–502.PubMedView ArticleGoogle Scholar
- Olsen LJ, Keegstra K. The binding of precursor proteins to chloroplasts requires nucleoside triphosphates in the intermembrane space. J Biol Chem. 1992;267(1):433–9.PubMedGoogle Scholar
- Olsen LJ, Theg SM, Selman BR, Keegstra K. ATP is required for the binding of precursor proteins to chloroplasts. J Biol Chem. 1989;264(12):6724–9.PubMedGoogle Scholar
- Young ME, Keegstra K, Froehlich JE. GTP promotes the formation of early-import intermediates but is not required during the translocation step of protein import into chloroplasts. Plant Physiol. 1999;121(1):237–44.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen YL, Chen LJ, Li HM. Polypeptide transport-associated domains of the Toc75 channel protein are located in the intermembrane space of chloroplasts. Plant Physiol. 2016;172(1):235–43.PubMedPubMed CentralView ArticleGoogle Scholar
- Paila YD, Richardson LG, Inoue H, Parks ES, McMahon J, Inoue K, et al. Multi-functional roles for the polypeptide transport associated domains of Toc75 in chloroplast protein import. Elife. 2016;5, e12631.PubMedPubMed CentralView ArticleGoogle Scholar
- O'Neil PK, Richardson LGL, Paila YD, Piszczek G, Chakravarthy S, Noinaj N, et al. The POTRA domains of Toc75 exhibit chaperone-like function to facilitate import into chloroplasts. Proc Natl Acad Sci U S A. 2017;114(24):E4868–76.PubMedView ArticleGoogle Scholar
- Kouranov A, Chen X, Fuks B, Schnell DJ. Tic20 and Tic22 are new components of the protein import apparatus at the chloroplast inner envelope membrane. J Cell Biol. 1998;143(4):991–1002.PubMedPubMed CentralView ArticleGoogle Scholar
- Glaser S, van Dooren GG, Agrawal S, Brooks CF, McFadden GI, Striepen B, et al. Tic22 is an essential chaperone required for protein import into the apicoplast. J Biol Chem. 2012;287(47):39505–12.PubMedPubMed CentralView ArticleGoogle Scholar
- Day PM, Potter D, Inoue K. Evolution and targeting of Omp85 homologs in the chloroplast outer envelope membrane. Front Plant Sci. 2014;5:535.PubMedPubMed CentralView ArticleGoogle Scholar
- Simmerman RF, Dave AM, Bruce BD. Structure and function of POTRA domains of Omp85/TPS superfamily. Int Rev Cell Mol Biol. 2014;308:1–34.PubMedView ArticleGoogle Scholar
- Kikuchi S, Bedard J, Hirano M, Hirabayashi Y, Oishi M, Imai M, et al. Uncovering the protein translocon at the chloroplast inner envelope membrane. Science. 2013;339(6119):571–4.PubMedView ArticleGoogle Scholar
- Kikuchi S, Oishi M, Hirabayashi Y, Lee DW, Hwang I, Nakai M. A 1-megadalton translocation complex containing Tic20 and Tic21 mediates chloroplast protein import at the inner envelope membrane. Plant Cell. 2009;21(6):1781–97.PubMedPubMed CentralView ArticleGoogle Scholar
- Kovacs-Bogdan E, Benz JP, Soll J, Bolter B. Tic20 forms a channel independent of Tic110 in chloroplasts. BMC Plant Biol. 2011;11:133.PubMedPubMed CentralView ArticleGoogle Scholar
- Teng YS, Su YS, Chen LJ, Lee YJ, Hwang I, Li HM. Tic21 is an essential translocon component for protein translocation across the chloroplast inner envelope membrane. Plant Cell. 2006;18(9):2247–57.PubMedPubMed CentralView ArticleGoogle Scholar
- Agne B, Kohler D, Baginsky S. Protein import-independent functions of Tic56, a component of the 1-MDa translocase at the inner chloroplast envelope membrane. Plant Signaling Behav. 2017;12(3), e1284726.View ArticleGoogle Scholar
- Kohler D, Helm S, Agne B, Baginsky S. Importance of translocon subunit Tic56 for rRNA processing and chloroplast ribosome assembly. Plant Physiol. 2016;172(4):2429–44.PubMedPubMed CentralView ArticleGoogle Scholar
- Inaba T, Li M, Alvarez-Huerta M, Kessler F, Schnell DJ. atTic110 functions as a scaffold for coordinating the stromal events of protein import into chloroplasts. J Biol Chem. 2003;278(40):38617–27.PubMedView ArticleGoogle Scholar
- Jackson DT, Froehlich JE, Keegstra K. The hydrophilic domain of Tic110, an inner envelope membrane component of the chloroplastic protein translocation apparatus, faces the stromal compartment. J Biol Chem. 1998;273(26):16583–8.PubMedView ArticleGoogle Scholar
- Kessler F, Blobel G. Interaction of the protein import and folding machineries of the chloroplast. Proc Natl Acad Sci U S A. 1996;93(15):7684–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Lubeck J, Soll J, Akita M, Nielsen E, Keegstra K. Topology of IEP110, a component of the chloroplastic protein import machinery present in the inner envelope membrane. EMBO J. 1996;15(16):4230–8.PubMedPubMed CentralGoogle Scholar
- Inaba T, Alvarez-Huerta M, Li M, Bauer J, Ewers C, Kessler F, et al. Arabidopsis tic110 is essential for the assembly and function of the protein import machinery of plastids. Plant Cell. 2005;17(5):1482–96.PubMedPubMed CentralView ArticleGoogle Scholar
- Kovacheva S, Bedard J, Patel R, Dudley P, Twell D, Rios G, et al. In vivo studies on the roles of Tic110, Tic40 and Hsp93 during chloroplast protein import. Plant J. 2005;41(3):412–28.PubMedView ArticleGoogle Scholar
- Chou ML, Chu CC, Chen LJ, Akita M, Li HM. Stimulation of transit-peptide release and ATP hydrolysis by a cochaperone during protein import into chloroplasts. J Cell Biol. 2006;175(6):893–900.PubMedPubMed CentralView ArticleGoogle Scholar
- Chou ML, Fitzpatrick LM, Tu SL, Budziszewski G, Potter-Lewis S, Akita M, et al. Tic40, a membrane-anchored co-chaperone homolog in the chloroplast protein translocon. EMBO J. 2003;22(12):2970–80.PubMedPubMed CentralView ArticleGoogle Scholar
- Theg SM, Bauerle C, Olsen LJ, Selman BR, Keegstra K. Internal ATP is the only energy requirement for the translocation of precursor proteins across chloroplastic membranes. J Biol Chem. 1989;264(12):6730–6.PubMedGoogle Scholar
- Constan D, Froehlich JE, Rangarajan S, Keegstra K. A stromal Hsp100 protein is required for normal chloroplast development and function in Arabidopsis. Plant Physiol. 2004;136(3):3605–15.PubMedPubMed CentralView ArticleGoogle Scholar
- Inoue H, Li M, Schnell DJ. An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts. Proc Natl Acad Sci U S A. 2013;110(8):3173–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Kovacheva S, Bedard J, Wardle A, Patel R, Jarvis P. Further in vivo studies on the role of the molecular chaperone, Hsp93, in plastid protein import. Plant J. 2007;50(2):364–79.PubMedView ArticleGoogle Scholar
- Su PH, Li HM. Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts. Plant Cell. 2010;22(5):1516–31.PubMedPubMed CentralView ArticleGoogle Scholar
- Tsai JY, Chu CC, Yeh YH, Chen LJ, Li HM, Hsiao CD. Structural characterizations of the chloroplast translocon protein Tic110. Plant J. 2013;75(5):847–57.PubMedPubMed CentralView ArticleGoogle Scholar
- Akita M, Nielsen E, Keegstra K. Identification of protein transport complexes in the chloroplastic envelope membranes via chemical cross-linking. J Cell Biol. 1997;136(5):983–94.PubMedPubMed CentralView ArticleGoogle Scholar
- Clarke AK. The chloroplast ATP-dependent Clp protease in vascular plants - new dimensions and future challenges. Physiol Plant. 2012;145(1):235–44.PubMedView ArticleGoogle Scholar
- Huang PK, Chan PT, Su PH, Chen LJ, Li HM. Chloroplast Hsp93 directly binds to transit peptides at an early stage of the preprotein import process. Plant Physiol. 2016;170(2):857–66.PubMedView ArticleGoogle Scholar
- Nielsen E, Akita M, Davila-Aponte J, Keegstra K. Stable association of chloroplastic precursors with protein translocation complexes that contain proteins from both envelope membranes and a stromal Hsp100 molecular chaperone. EMBO J. 1997;16(5):935–46.PubMedPubMed CentralView ArticleGoogle Scholar
- Rosano GL, Bruch EM, Ceccarelli EA. Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana. J Biol Chem. 2011;286(34):29671–80.PubMedPubMed CentralView ArticleGoogle Scholar
- Liu L, McNeilage RT, Shi LX, Theg SM. ATP requirement for chloroplast protein import is set by the Km for ATP hydrolysis of stromal Hsp70 in Physcomitrella patens. Plant Cell. 2014;26(3):1246–55.PubMedPubMed CentralView ArticleGoogle Scholar
- Chotewutmontri P, Bruce BD. Non-native, N-terminal Hsp70 molecular motor recognition elements in transit peptides support plastid protein translocation. J Biol Chem. 2015;290(12):7602–21.PubMedPubMed CentralView ArticleGoogle Scholar
- Li M, Schnell DJ. Reconstitution of protein targeting to the inner envelope membrane of chloroplasts. J Cell Biol. 2006;175(2):249–59.PubMedPubMed CentralView ArticleGoogle Scholar
- Nishimura K, Kato Y, Sakamoto W. Chloroplast proteases: updates on proteolysis within and across suborganellar compartments. Plant Physiol. 2016;171(4):2280–93.PubMedPubMed CentralGoogle Scholar
- Baker TA, Sauer RT. ATP-dependent proteases of bacteria: recognition logic and operating principles. Trends Biochem Sci. 2006;31(12):647–53.PubMedPubMed CentralView ArticleGoogle Scholar
- Nishimura K, Kato Y, Sakamoto W. Essentials of proteolytic machineries in chloroplasts. Mol Plant. 2017;10(1):4–19.PubMedView ArticleGoogle Scholar
- Flores-Perez U, Bedard J, Tanabe N, Lymperopoulos P, Clarke AK, Jarvis P. Functional analysis of the Hsp93/ClpC chaperone at the chloroplast envelope. Plant Physiol. 2016;170(1):147–62.PubMedView ArticleGoogle Scholar
- Sjogren LL, Tanabe N, Lymperopoulos P, Khan NZ, Rodermel SR, Aronsson H, et al. Quantitative analysis of the chloroplast molecular chaperone ClpC/Hsp93 in Arabidopsis reveals new insights into its localization, interaction with the Clp proteolytic core, and functional importance. J Biol Chem. 2014;289(16):11318–30.PubMedPubMed CentralView ArticleGoogle Scholar
- Tu CJ, Schuenemann D, Hoffman NE. Chloroplast FtsY, chloroplast signal recognition particle, and GTP are required to reconstitute the soluble phase of light-harvesting chlorophyll protein transport into thylakoid membranes. J Biol Chem. 1999;274(38):27219–24.PubMedView ArticleGoogle Scholar
- Li X, Henry R, Yuan J, Cline K, Hoffman NE. A chloroplast homologue of the signal recognition particle subunit SRP54 is involved in the posttranslational integration of a protein into thylakoid membranes. Proc Natl Acad Sci U S A. 1995;92(9):3789–93.PubMedPubMed CentralView ArticleGoogle Scholar
- Falk S, Sinning I. cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a, b-binding proteins. J Biol Chem. 2010;285(28):21655–61.PubMedPubMed CentralView ArticleGoogle Scholar
- Ziehe D, Dunschede B, Schunemann D. From bacteria to chloroplasts: evolution of the chloroplast SRP system. Biol Chem. 2017;398(5-6):653–61.PubMedView ArticleGoogle Scholar
- Falk S, Ravaud S, Koch J, Sinning I. The C terminus of the Alb3 membrane insertase recruits cpSRP43 to the thylakoid membrane. J Biol Chem. 2010;285(8):5954–62.PubMedView ArticleGoogle Scholar
- Moore M, Harrison MS, Peterson EC, Henry R. Chloroplast Oxa1p homolog albino3 is required for post-translational integration of the light harvesting chlorophyll-binding protein into thylakoid membranes. J Biol Chem. 2000;275(3):1529–32.PubMedView ArticleGoogle Scholar
- Schuenemann D, Gupta S, Persello-Cartieaux F, Klimyuk VI, Jones JD, Nussaume L, et al. A novel signal recognition particle targets light-harvesting proteins to the thylakoid membranes. Proc Natl Acad Sci U S A. 1998;95(17):10312–6.PubMedPubMed CentralView ArticleGoogle Scholar
- Franklin AE, Hoffman NE. Characterization of a chloroplast homologue of the 54-kDa subunit of the signal recognition particle. J Biol Chem. 1993;268(29):22175–80.PubMedGoogle Scholar
- Ouyang M, Li X, Ma J, Chi W, Xiao J, Zou M, et al. LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway. Nature Commun. 2011;2:277.View ArticleGoogle Scholar
- Bedard J, Trosch R, Wu F, Ling Q, Flores-Perez U, Topel M, et al. New suppressors of the chloroplast protein import mutant tic40 reveal a genetic link between protein import and thylakoid biogenesis. Plant Cell. 2017;29(7):1726–47.PubMedView ArticleGoogle Scholar
- Gerdes L, Bals T, Klostermann E, Karl M, Philippar K, Hunken M, et al. A second thylakoid membrane-localized Alb3/OxaI/YidC homologue is involved in proper chloroplast biogenesis in Arabidopsis thaliana. J Biol Chem. 2006;281(24):16632–42.PubMedView ArticleGoogle Scholar
- Skretas G, Georgiou G. Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli. Appl Environ Microbiol. 2010;76(17):5852–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Trosch R, Topel M, Flores-Perez U, Jarvis P. Genetic and physical interaction studies reveal functional similarities between ALBINO3 and ALBINO4 in Arabidopsis. Plant Physiol. 2015;169(2):1292–306.PubMedPubMed CentralView ArticleGoogle Scholar
- Zoschke R, Barkan A. Genome-wide analysis of thylakoid-bound ribosomes in maize reveals principles of cotranslational targeting to the thylakoid membrane. Proc Natl Acad Sci U S A. 2015;112(13):E1678–87.PubMedPubMed CentralView ArticleGoogle Scholar
- Drescher A, Ruf S, Calsa Jr T, Carrer H, Bock R. The two largest chloroplast genome-encoded open reading frames of higher plants are essential genes. Plant J. 2000;22(2):97–104.PubMedView ArticleGoogle Scholar
- Skalitzky CA, Martin JR, Harwood JH, Beirne JJ, Adamczyk BJ, Heck GR, et al. Plastids contain a second sec translocase system with essential functions. Plant Physiol. 2011;155(1):354–69.PubMedView ArticleGoogle Scholar
- Li Y, Singhal R, Taylor IW, McMinn PH, Chua XY, Cline K, et al. The Sec2 translocase of the chloroplast inner envelope contains a unique and dedicated SECE2 component. Plant J. 2015;84(4):647–58.PubMedView ArticleGoogle Scholar
- Li Y, Martin JR, Aldama GA, Fernandez DE, Cline K. Identification of putative substrates of SEC2, a chloroplast inner envelope translocase. Plant Physiol. 2017;173(4):2121–37.PubMedPubMed CentralView ArticleGoogle Scholar
- Bohnert M, Rehling P, Guiard B, Herrmann JM, Pfanner N, van der Laan M. Cooperation of stop-transfer and conservative sorting mechanisms in mitochondrial protein transport. Curr Biol. 2010;20(13):1227–32.PubMedView ArticleGoogle Scholar