- Open Access
Hsp70 at the membrane: driving protein translocation
BMC Biologyvolume 16, Article number: 11 (2018)
Efficient movement of proteins across membranes is required for cell health. The translocation process is particularly challenging when the channel in the membrane through which proteins must pass is narrow—such as those in the membranes of the endoplasmic reticulum and mitochondria. Hsp70 molecular chaperones play roles on both sides of these membranes, ensuring efficient translocation of proteins synthesized on cytosolic ribosomes into the interior of these organelles. The “import motor” in the mitochondrial matrix, which is essential for driving the movement of proteins across the mitochondrial inner membrane, is arguably the most complex Hsp70-based system in the cell.
Challenges in protein translocation across membranes
Proteins synthesized on cytosolic ribosomes and translocated across membranes into organelles play critical roles in cell and organismal physiology. Translocation of proteins into the endoplasmic reticulum (ER) and mitochondria is especially demanding. The protein complexes embedded in the membrane, referred to as translocases or translocons, through which the proteins must pass, have narrow channels [1, 2]. They are able to accommodate only a completely unfolded chain or, at most, an α-helix. Thus, postponing folding, yet preventing aggregation, of a protein is necessary for its efficient translocation. In addition, protein movement must not only be vectorial, that is, unidirectional from the cytosol into the organelle, it must also be efficient to keep up with the heavy cellular demand for organelle function.
For many ER proteins, the co-translational nature of the translocation process overcomes such hurdles. Coupling of protein translation and protein translocation minimizes the issue of tertiary structure hindering passage through the translocation channel, while using the “force” of protein synthesis to drive directional movement across the membrane. Via action of signal recognition particle (SRP) binding to targeting sequences at the N-terminus of an ER-destined protein, the translating ribosome docks directly onto the translocon of the ER membrane [3, 4]. This precise docking provides a direct conduit for the nascent polypeptide chain from the ribosome exit tunnel through the channel in the membrane-imbedded translocon . However, in organisms as diverse as budding yeast (Saccharomyces cerevisiae) and humans, a substantial number of proteins are translocated post-translationally into the ER . Moreover, mitochondria have no exact analog of the SRP system that results in a direct physical connection between the ribosome and the translocon of the outer mitochondrial membrane.
Hsp70 molecular chaperones function both in the cytosol and internally on the luminal/matrix face of ER/mitochondrial membranes, helping cells overcome these inherent challenges of protein translocation across membranes. In this review, particular attention is given to the Hsp70 system of the mitochondrial matrix, which is required for the translocation of all nuclear-encoded proteins into this subcompartment . Many fundamental aspects of both ER and mitochondrial translocation systems have been highly conserved in evolution. Throughout, S. cerevisiae nomenclature is used as much of the work to understand the mechanism of protein import and molecular chaperone function was performed using this model organism.
Properties of Hsp70s critical for cellular functions
Hsp70 molecular chaperones are present in all major cellular compartments (i.e., cytosol, nucleus, ER, and mitochondria), functioning in diverse cellular processes from protein folding to disassembly of protein complexes to protein translocation across membranes. While the protein translocation is the focus of this review, Hsp70s, when involved in any of these processes, bind to seven-residue segments of polypeptide that are overall hydrophobic in nature . Virtually every protein that is not folded into its native state has multiple accessible Hsp70 binding sites, because residues found in the hydrophobic core in the native conformation are exposed. It has been estimated that most proteins have an Hsp70 binding site every 30–40 residues .
Cycles of interaction with substrate polypeptides is an important aspect of Hsp70 function, not only in protein translocation, but in other functions such as protein folding and disassembly of protein complexes. Hsp70–substrate interactions are controlled by ATP binding and hydrolysis (Fig. 1) . When ATP is bound to Hsp70, the substrate on-rate is very rapid, but so is the off-rate. ATP-hydrolysis results in trapping the substrate polypeptide, and nucleotide exchange results in rapid dissociation. Two types of co-chaperones regulate the Hsp70–substrate interaction cycle . One, J-proteins, via the action of their highly conserved J-domain, stimulates ATP hydrolysis and thus stabilization of substrate interaction. The other, nucleotide exchange factors (NEFs), drives exchange of ADP for ATP, facilitating substrate release.
Although these fundamental principles apply to all Hsp70 systems, specialization is common. An Hsp70 typically has multiple different J-protein partners, which may either target Hsp70 to a particular site within a compartment or bind a substrate itself, targeting it to the Hsp70 . For example, the single Hsp70 of the ER partners with six J-proteins. Also, Hsp70s themselves may have specialized interactions, independent of their substrate binding, that render them more effective in specific cellular roles, including protein translocation. For example, the major cytosolic Hsp70s of all eukaryotes (called Ssa in S. cerevisiae and Hsc70/Hsp70 in metazoans) have a conserved EEVD tetrapeptide at their C-terminus, serving to target them to particular binding partners , including receptors at the membrane, as described below. In addition, although most metazoans have only the Hsc70/Hsp70 type of Hsp70 in the cytosol, fungi have a second type, called Ssb [13, 14]. Both Ssa and Ssb Hsp70s are involved in protein translocation across membranes (Fig. 2).
Routes to the mitochondria and ER involving Hsp70 action
To reach their destination, ER and mitochondrial proteins utilize a variety of translocation pathways. For example, all nuclear-encoded proteins destined for the mitochondrial matrix pass through two translocons, the TOM complex of the outer membrane and the TIM23 complex of the inner membrane  (Fig. 3). Integral mitochondrial inner membrane proteins, which also utilize the TOM translocon of the outer membrane, are laterally transferred into the inner membrane via one of two inner membrane translocases: the TIM22 translocon, which is dedicated to integral membrane proteins, or the TIM23 translocon. The TIM23 route is often called the “presequence pathway”, because the proteins utilizing this pathway, whether they end up wholly within the matrix or in the inner membrane, are typically synthesized with N-terminal targeting sequences (presequences) that are cleaved in the matrix. As discussed below, the TIM22 pathway is particularly dependent on cytosolic Hsp70s, while the translocation of proteins into the matrix via the presequence (TIM23) pathway requires matrix Hsp70 activity. To reach the ER lumen, many proteins utilize the SRP pathway through the SEC61 translocon of the ER membrane. This route does not require Hsp70 action (Fig. 3). However, some, particularly short lumenal polypeptides or those with less effective targeting sequences often do not bind SRP, but rather are translocated post-translationally through SEC61, relying heavily on cytosolic Hsp70 (and also lumenal Hsp70; see below).
Hsp70 on the cytosolic side of the membrane
Data obtained in the 1990s pointed to a role for cytosolic Hsp70 chaperones in the translocation of proteins into both the ER and mitochondria [15,16,17,18]. The idea put forth at the time, and consistent with emerging in vitro evidence that chaperones help prevent aggregation of unfolded proteins , was that binding prevents formation of tertiary structure that hinders threading of the protein through narrow translocation channels. Overall, work over the ensuing years has supported this general idea. Recent data have brought both clarification and evidence of unanticipated complexity. On one hand, the issue of aggregation of proteins destined for the mitochondria is likely not as extreme as originally envisioned. Translation of many proteins destined for mitochondria is now known to occur at the mitochondrial outer membrane, in close proximity to the TOM complex [20, 21], rather than in the bulk cytosol, as previously thought. On the other hand, post-translational translocation into the ER via mechanisms not dependent on SRP are more common than previously appreciated . As described below, besides helping to maintain proteins in a partially folded, yet soluble state, Hsp70 binding targets substrates to the mitochondrial outer membrane and ER translocon channels. In both cases the C-terminal EEVD tetrapeptide of Hsp70 is involved in the targeting.
The outer mitochondrial membrane TOM complex is composed of channel-forming Tom40 and associated proteins, including the two receptor proteins Tom20 and Tom70 (Fig. 4). Tom70 is the primary receptor for proteins that have internal hydrophobic targeting sequences, such as the abundant, integral inner membrane carrier proteins that utilize the TIM22 inner membrane translocon (e.g., ATP/ADP carrier) [23, 24]. In addition, Tom70 binds Hsp70’s C-terminal EEVD via its tetratricopeptide repeat (TPR) domain . This dual interaction is likely regulatory, with conformational changes upon EEVD binding linking receptor activation to chaperone binding. Tom20, the primary receptor for the presequence pathway, does not have an EEVD binding site. Perhaps the challenge of preventing aggregation of abundant integral membrane proteins was behind evolution of direct chaperone–receptor interactions. In mammalian cells, but not yeast cells, the molecular chaperone Hsp90 acts similarly, interacting with the TPR domain of the Tom70 receptor through its conserved C-terminal EEVD [26, 27].
During post-translational translocation into the ER, the EEVD of Ssa Hsp70 interacts with the TPR domain of Sec72, a SEC61 translocon-associated protein  (Fig. 4). Though lacking a C-terminal EEVD tetrapeptide, Ssb Hsp70s, which associate with ribosomes near the exit of the tunnel, also interact with Sec72 . This interaction occurs via Ssb’s N-terminal nucleotide binding domain. Metazoans do not have a Sec72 homolog, but mammals have a second J-protein, ERj1 (DnaJC1) in the ER membrane. The lumenal J-domain functions in protein translocation across the membrane. A cytosolic domain binds ribosomes near the tunnel exit site , and may help recruit them to the ER membrane.
Analysis of individual proteins gives substantial support to the idea that the Ssa Hsp70 class plays a significant role in post-translational translocation [30,31,32,33]. For Ssb Hsp70s, recent in vivo selective ribosome profiling data provide genome-wide insights into the breadth of its nascent chain interactions . On the order of 80% of the different nascent chains known to be destined for mitochondria were found to bind to Ssb, consistent with observed aggregation of mitochondrial proteins in cells lacking Ssb  and the ability of increased expression of Ssb to overcome the growth defect caused by inefficient mitochondrial protein translocation . Ssb also interacts with almost half of all the different ER proteins. While most of these interactors do not require SRP for ER targeting (e.g., tail anchored proteins ), Ssb also binds a significant number of proteins known to transit into the ER via the SRP-dependent mechanism. In these, the first Ssb binding site to emerge from the ribosome is typically more N-terminal than the SRP binding site . Many questions remain. Does an individual nascent chain bind both Ssb and SRP? Is there a mechanistic cooperation between Ssb and SRP, perhaps a handing-over from one system to the other? Or is this binding indicative of alternative pathways, SRP-dependent and SRP-independent?
Hsp70 on the matrix/lumenal side of the membrane
Hsp70s in the mitochondrial matrix and the ER lumen play a critical and more active role in protein translocation than do those on the cytosolic side. They form the core of the machinery, often called “import motors”, that binds the translocating polypeptide and drives it across the membrane. Hsp70s of both these import motors utilize the same biochemical properties to drive translocation as used by Hsp70s when functioning in other biological processes—initial interaction of Hsp70-ATP with substrate, stabilized by J-protein driven ATP hydrolysis, then destabilized by NEF-driven nucleotide exchange. ER lumenal Hsp70 (officially Kar2 in yeast; but often called BiP in both yeast and metazoans) drives post-translational import of proteins through the Sec61 channel [1, 5]. Sec63 is this motor’s dedicated J-protein; it associates with the SEC61 complex, as a component of the Sec62/63 complex, which in yeast also includes Sec71/72. PAM, the mitochondrial presequence associated motor of the matrix, provides the driving force for movement of all nuclear-encoded matrix proteins [2, 6, 38]. Below I concentrate on mitochondrial PAM, as it has been studied much more extensively than the lumenal ER Hsp70 system.
Steps of the presequence import pathway into the mitochondrial matrix
Before PAM can act, the N-terminus of the preprotein must enter the matrix. The N-terminal targeting presequence, an antipathic α-helix, interacts with a series of receptors as it moves from the cytosolic surface of the outer membrane to Tim23 complex in the intermembrane space . It first interacts with the Tom20 receptor, then other components of the TOM complex, then components of the TIM23 complex. The membrane potential, negative on the matrix side, drives the positively charged presequence across the membrane. Translocation of the remainder of the polypeptide requires the action of the import motor, PAM (Fig. 5a).
The TIM23 complex contains three essential proteins. Two, Tim23 and Tim17, are related integral membrane proteins (Fig. 5b). Tim23 forms the channel; Tim17 likely plays a role in maintaining the translocon’s structure and channel gating [39,40,41]. Both have four transmembrane helices, with the N- and C-termini extending into the intermembrane space. Two loops (1 and 3) between the membrane spanning segments, on the order of 23 and 10 residues, respectively, extend into the matrix and serve as interaction sites for PAM. More than one molecule of both Tim23 and Tim17 are present in each TIM23 complex; the exact number is not known, a complicating factor in understanding the mechanism of action of the import motor. The third essential subunit is Tim50. Both Tim50 and Tim23 have domains that extend into the intermembrane space and interact with the targeting sequence, promoting the first step of translocation across the inner membrane, that is, opening of the gated Tim23 channel [42, 43].
The TIM23 complex is also responsible for the transport of some inner membrane proteins (Fig. 2). For many of these, PAM is not involved. Rather, the membrane potential drives the presequence targeting sequence through the channel; an adjacent “stop-transfer” sequence arrests movement and facilitates lateral transfer into the membrane [6, 44]. But in other cases, proteins are first imported into the matrix, then insertion into the inner membrane is facilitated by the action of the protein insertion machinery, called the oxidase assembly (OXA) system . This process is often referred to as conservative sorting because of its resemblance to transport systems of bacteria, the progenitor of mitochondria . In a few cases, the PAM/OXA system is used for some of a protein’s transmembrane domains, but the stop-transfer, lateral gating system for others [47, 48].
Architecture of the presequence associated motor PAM
PAM is composed of six subunits (Fig. 5), five of which are essential. Three are core Hsp70 system essential components—Hsp70 Ssc1, J-protein Pam18 (also called Tim14), and NEF Mge1 [6, 38]. The Hsp70 and NEF are the same molecules that carry out other processes in the mitochondrial matrix, including general protein folding and remodeling of protein complexes . However, J-protein Pam18, like its analog Sec63 in the ER lumen, is specific for protein translocation. When Ssc1 and Mge1 engage in other biological processes, they work with a different J-protein, such as Mdj1 [50, 51]. The two other essential components are Pam16 (also called Tim16) [52,53,54] and Tim44. Tim44 is considered the “hub” of the motor. It serves as the connector between the motor and the translocon, interacting with the TIM23 complex and with other motor components [55, 56]. The nonessential motor component Pam17, a membrane protein having a matrix domain, appears to play a yet-to-be clearly defined early role [57,58,59].
The architecture of PAM is complex (Fig. 5). Pam18 and Pam16, in addition to Tim44, have multiple interactions that provide functional redundancy and robustness. J-protein Pam18 has a single transmembrane segment. On the intermembrane space side of the membrane it interacts with Tim17 , and on the matrix side with Pam16 [61,62,63,64]. In turn, Pam16, via its N-terminus, interacts with Tim44 [63, 65]. Pam16 has a degenerate J-like domain, incapable of stimulating Hsp70’s ATPase activity. Rather, along with adjacent residues, the J-like domain interacts with Pam18’s J-domain [61, 62]. These interactions on both sides of the membrane are important for Pam18’s association with the TIM23 translocon .
The hub protein Tim44, a peripheral membrane protein, has two domains of approximately equal size (Figs. 5 and 6). The N-terminal domain (NTD) is intrinsically disordered ; the C-terminal domain (CTD) forms an α + β barrel with two N-terminal α-helices protruding from the core , which are thought to be involved in membrane association . The NTD serves as the site of binding for both Hsp70 and Pam16 [65, 69]. Pam16 interacts with a small segment near the N-terminus . Interaction with Hsp70 is likely more dispersed over the NTD, as both Hsp70 domains are involved in the Hsp70–Tim44 interaction [70,71,72,73]. The primary role of Tim44’s CTD is to interact with the TIM23 complex. Site-specific crosslinking indicates that adjacent patches on a face of the barrel interact with the TIM23 complex—one with Tim17 and one with Tim23 (Fig. 6). The large matrix-exposed loop of Tim17 (loop 1) interacts with one CTD patch and the small loop (loop 3) of Tim17 with the other [66, 74]. Loop 1 of Tim17 crosslinks to Pam17, the nonessential PAM component .
But the Tim44 picture is not as “simple” as the NTD interacting with the motor and the CTD with the translocon. Loop 1 of Tim23 also crosslinks to Tim44’s NTD . This complex crosslinking pattern is consistent with the ability of the two domains to support viability when expressed separately (i.e., in trans) ; but such yeast cells grow very slowly, underscoring the complexity of Tim44 function. However, as Tim23’s loop 1 is only 24 residues, it is unlikely that both domains interact with the same Tim23 molecule simultaneously . Thus, whether one Tim44 molecule interacts with two different Tim23 molecules simultaneously or interactions of the CTD and NTD occur sequentially to one Tim23 molecule remains an open question, leaving unresolved important mechanistic and regulatory questions raised below. That the stoichiometry of Tim23 in the TIM23 complex is also unresolved and that Tim44 has been reported to be a dimer further confound a mechanistic understanding of PAM function .
How does an import motor mechanistically drive efficient protein translocation?
For many years two challenging questions have vexed workers studying PAM: what mechanistic principle(s) are behind motor function, and what motor characteristics drive motor efficiency? Two models of import motor action were put forward soon after it became clear that Hsp70 was required for post-translational translocation across membranes—“brownian (molecular) ratchet” and “power stroke” [77,78,79]. The mechanism by which Hsp70 binding to the translocating polypeptide drives directional movement is the fundamental difference between the two models. In the power stroke model, the polypeptide chain is pulled into the matrix by Hsp70 acting as a lever arm to generate force through conformational change, with Tim44 serving as a fulcrum. In the simplest form of the Brownian motion model, binding of Hsp70 to the translocating polypeptide prevents its backsliding because of its large size compared to the narrow import channel. Each model was appealing, yet problematic, in its own way. The power stroke model, as envisioned, helped rationalize data showing that Hsp70 binding not only drove translocation of an unfolded polypeptide into the matrix, but generated sufficient power to unfold a protein domain “stuck” at the outer membrane [80,81,82]. But whether Hsp70’s conformational changes are of sufficient magnitude to move the chain through the channel has not been critically addressed. On the other hand, the simplicity of the ratchet model was appealing. Indeed, early studies using an in vitro ER system showed Hsp70 BiP and J-protein Sec63 to be sufficient to move preproalpha factor, a small protein that is efficiently translocated post-translationally in vivo, through the SEC61 translocon . However, it was difficult to envision how simply preventing backsliding would suffice energetically for more challenging substrates.
An extension of the Brownian motion model, grounded in the more thorough consideration of the effects of binding of a large molecule such as Hsp70 to a translocating polypeptide close to the channel, has been developed . According to this “entropic pulling” model, binding of Hsp70 at the exit pore generates a force, because “simple” restriction of its movement—“bumping into” the membrane or translocon—generates energy (i.e., a pulling force) (Fig. 7). The appeal of this model is that “simply” binding Hsp70 could generate a force without the need for either a fulcrum or a conformational change of a magnitude required to drive translocation at a biologically reasonable rate. Rather, “just” cycles of binding of Hsp70s to the incoming polypeptide could be sufficient. Similar force generation considerations arise when considering Hsp70 functioning in remodeling of protein complexes and dissolution of protein aggregates. Recent observation of uncoating of clathrin cages by Hsp70 and the J-protein auxilin are consistent with an entropic pulling model . The juxtaposition of the auxilin and Hsp70 binding sites were critical; when moved further apart, the efficiency of the uncoating reaction decreased significantly. In addition, when an immunoglobulin binding site was placed at an appropriate position, addition of immunoglobulin alone facilitates cage disassembly.
For polypeptide translocation, the issues are more complex than uncoating clathrin cages. Not only must Hsp70 binding occur very close to the channel, but also a series of Hsp70 molecules must interact in rapid succession, each as close to the channel as possible. Interactions of Tim44 with motor components and with the translocon could serve both functions. Tim44 serves to bridge the interactions between Tim23/17 and both Hsp70 and J-protein Pam18 (via Pam16). In addition, binding of substrate by Hsp70 destabilizes its interaction with Tim44 [73, 86], thereby allowing binding of another Hsp70, and continuation of translocation (Fig. 6). For the motor to function efficiently, Hsp70 at the channel must be in the ATP-, not ADP-, bound state, to initiate interaction with the incoming polypeptide rapidly. Premature stimulation of ATP hydrolysis by the J-protein Pam18 in the absence of substrate (i.e., the translocating polypeptide) could occur, decreasing motor efficiency. But on the other hand, efficient motor function also requires rapid J-domain action as soon as the translocating polypeptide enters the matrix.
Discussion on the issue of keeping Hsp70 primed, in the ATP-state, has centered around Tim44’s interactions with multiple binding partners and the Pam18–Pam16 heterodimer. The idea that Tim44 may play an important role was boosted by the findings that the intrinsically disordered NTD binds preprotein targeting sequences [66, 87] in addition to Pam16/18 and Hsp70. Many scaffolding proteins involved in signal transduction and regulation  are intrinsically disordered, having different conformations, depending upon which of their binding partners they are interacting with. The idea that such conformational changes play a role in regulating motor function became more intriguing with the finding that the site to which the targeting sequence binds overlaps with residues important for binding of Hsp70 and Tim23  (Fig. 6a). This made it tempting to speculate that binding of the targeting sequence at this site, upon entrance into the matrix, induces a conformational change in Tim44 NTD that “activates” the motor. Perhaps conformational changes in Tim44 bring the Pam18 J-domain in close proximity to its binding site on Hsp70 [66, 89]. On the other hand, the Pam16–Pam18 interaction interface may be altered in some way. The idea that interaction of Pam18 with Pam16 may regulate Pam18’s ability to stimulate Hsp70’s ATPase activity stems from the observation that the Pam16–Pam18 heterodimer has on the order of 50% of the stimulatory ability of Pam18 alone [61, 90]. However, Pam18 variants having substitutions that reduce activity more than this support efficient mitochondrial import and robust cell growth . Also, a Pam18–Pam16 heterodimer of J- and J-like domain-containing fragments was found to be inactive on stimulation of Hsp70’s ATPase activity . While this inactivity could be indicative of a regulatory function, it could also be due to the absence of adjacent sequences shown to be important for forming an active complex . Thus, although the Pam18–Pam16 interaction is central to motor function and Tim44 has characteristics consistent with regulatory roles, it remains unresolved how either facilitates maintenance of the motor in a state primed for action.
As described above, considerable progress has been made towards understanding the action of Hsp70s in protein translocation on both sides of membranes. Many questions remain, however. On the cytosolic side of the membranes, as results of more genome-wide ribosome profiling studies become available, a better picture of Hsp70 interactions with nascent chains will develop, allowing more directed studies to understand the importance of these interactions. Also, as new information about organelle targeting systems emerge, it will be interesting to see how generally Hsp70 functions on the cytosolic side. For example, do Hsp70s play a role in the recently identified SND targeting system to the ER that uses the SEC61 translocon ? Regarding import motor function, clearly more detailed knowledge is needed to gain a mechanistic understanding not only of how this molecular machine acts but also how such efficiency is obtained. Unfortunately, the mitochondrial inner membrane translocases have been difficult to purify and resistant to structural analysis. Hopefully, the rapid advancements occurring in structural biology and single molecule approaches that have recently provided insight into the Tom40 translocon and Hsp70s [93, 94] will soon be productive for the TIM23 translocon and PAM as well.
Rapoport TA, Li L, Park E. Structural and mechanistic insights into protein translocation. Annu Rev Cell Dev Biol. 2017;33:369–90.
Wiedemann N, Pfanner N. Mitochondrial machineries for protein import and assembly. Annu Rev Biochem. 2017;86:685–714.
Walter P, Lingappa VR. Mechanism of protein translocation across the endoplasmic reticulum membrane. Annu Rev Cell Biol. 1986;2:499–516.
Voorhees RM, Hegde RS. Toward a structural understanding of co-translational protein translocation. Curr Opin Cell Biol. 2016;41:91–9.
Aviram N, Schuldiner M. Embracing the void--how much do we really know about targeting and translocation to the endoplasmic reticulum? Curr Opin Cell Biol. 2014;29:8–17.
Schulz C, Schendzielorz A, Rehling P. Unlocking the presequence import pathway. Trends Cell Biol. 2015;25:265–75.
Clerico EM, Tilitsky JM, Meng W, Gierasch LM. How hsp70 molecular machines interact with their substrates to mediate diverse physiological functions. J Mol Biol. 2015;427:1575–88.
Rudiger S, Buchberger A, Bukau B. Interaction of Hsp70 chaperones with substrates. Nat Struct Biol. 1997;4:342–9.
Kityk R, Vogel M, Schlecht R, Bukau B, Mayer MP. Pathways of allosteric regulation in Hsp70 chaperones. Nat Commun. 2015;6:8308.
Kampinga HH, Craig EA. The HSP70 chaperone machinery: J proteins as drivers of functional specificity. Nat Rev Mol Cell Biol. 2010;11:579–92.
Craig EA, Marszalek J. How do J-proteins get Hsp70 to do so many different things? Trends Biochem Sci. 2017;42:355–68.
Assimon VA, Southworth DR, Gestwicki JE. Specific binding of tetratricopeptide repeat proteins to heat shock protein 70 (Hsp70) and heat shock protein 90 (Hsp90) is regulated by affinity and phosphorylation. Biochemistry. 2015;54:7120–31.
Zhang Y, Sinning I, Rospert S. Two chaperones locked in an embrace: structure and function of the ribosome-associated complex RAC. Nat Struct Mol Biol. 2017;24:611–9.
Kominek J, Marszalek J, Neuveglise C, Craig EA, Williams BL. The complex evolutionary dynamics of Hsp70s: a genomic and functional perspective. Genome Biol Evol. 2013;5:2460–77.
Becker J, Walter W, Yan W, Craig EA. Functional interaction of cytosolic hsp70 and a DnaJ-related protein, Ydj1p, in protein translocation in vivo. Mol Cell Biol. 1996;16:4378–86.
Terada K, Kanazawa M, Bukau B, Mori M. The human DnaJ homologue dj2 facilitates mitochondrial protein import and luciferase refolding. J Cell Biol. 1997;139:1089–95.
Chirico WJ, Waters MG, Blobel G. 70 K heat shock related proteins stimulate protein translocation into microsomes. Nature. 1988;332:805–10.
Deshaies RJ, Koch BD, Werner-Washburne M, Craig EA, Schekman R. A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature. 1988;332:800–5.
Schroder H, Langer T, Hartl FU, Bukau B. DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage. EMBO J. 1993;12:4137–44.
Williams CC, Jan CH, Weissman JS. Targeting and plasticity of mitochondrial proteins revealed by proximity-specific ribosome profiling. Science. 2014;346:748–51.
Gold VA, Chroscicki P, Bragoszewski P, Chacinska A. Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. EMBO Rep. 2017;18(10):1786–800.
Ast T, Schuldiner M. All roads lead to Rome (but some may be harder to travel): SRP-independent translocation into the endoplasmic reticulum. Crit Rev Biochem Mol Biol. 2013;48:273–88.
Wu Y, Sha B. Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p. Nat Struct Mol Biol. 2006;13:589–93.
Wiedemann N, Pfanner N, Ryan MT. The three modules of ADP/ATP carrier cooperate in receptor recruitment and translocation into mitochondria. EMBO J. 2001;20:951–60.
Li J, Qian X, Hu J, Sha B. Molecular chaperone Hsp70/Hsp90 prepares the mitochondrial outer membrane translocon receptor Tom71 for preprotein loading. J Biol Chem. 2009;284:23852–9.
Young JC, Hoogenraad NJ, Hartl FU. Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70. Cell. 2003;112:41–50.
Zanphorlin LM, Lima TB, Wong MJ, Balbuena TS, Minetti CA, Remeta DP, et al. Heat shock protein 90 kDa (Hsp90) has a second functional interaction site with the mitochondrial import receptor Tom70. J Biol Chem. 2016;291:18620–31.
Tripathi A, Mandon EC, Gilmore R, Rapoport TA. Two alternative binding mechanisms connect the protein translocation Sec71-Sec72 complex with heat shock proteins. J Biol Chem. 2017;292:8007–18.
Blau M, Mullapudi S, Becker T, Dudek J, Zimmermann R, Penczek PA, et al. ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane. Nat Struct Mol Biol. 2005;12:1015–6.
Chartron JW, Gonzalez GM, Clemons Jr WM. A structural model of the Sgt2 protein and its interactions with chaperones and the Get4/Get5 complex. J Biol Chem. 2011;286:34325–34.
Rabu C, Wipf P, Brodsky JL, High S. A precursor-specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum. J Biol Chem. 2008;283:27504–13.
Ast T, Cohen G, Schuldiner M. A network of cytosolic factors targets SRP-independent proteins to the endoplasmic reticulum. Cell. 2013;152:1134–45.
Ngosuwan J, Wang NM, Fung KL, Chirico WJ. Roles of cytosolic Hsp70 and Hsp40 molecular chaperones in post-translational translocation of presecretory proteins into the endoplasmic reticulum. J Biol Chem. 2003;278:7034–42.
Doring K, Ahmed N, Riemer T, Suresh HG, Vainshtein Y, Habich M, et al. Profiling Ssb-nascent chain interactions reveals principles of Hsp70-assisted folding. Cell. 2017;170:298–311. e20.
Willmund F, del Alamo M, Pechmann S, Chen T, Albanese V, Dammer EB, et al. The cotranslational function of ribosome-associated Hsp70 in eukaryotic protein homeostasis. Cell. 2013;152:196–209.
Wang X, Chen XJ. A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death. Nature. 2015;524:481–4.
Hegde RS, Keenan RJ. Tail-anchored membrane protein insertion into the endoplasmic reticulum. Nat Rev Mol Cell Biol. 2011;12:787–98.
Marom M, Azem A, Mokranjac D. Understanding the molecular mechanism of protein translocation across the mitochondrial inner membrane: still a long way to go. Biochim Biophys Acta. 2011;1808:990–1001.
Martinez-Caballero S, Grigoriev SM, Herrmann JM, Campo ML, Kinnally KW. Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23. J Biol Chem. 2007;282:3584–93.
Ramesh A, Peleh V, Martinez-Caballero S, Wollweber F, Sommer F, van der Laan M, et al. A disulfide bond in the TIM23 complex is crucial for voltage gating and mitochondrial protein import. J Cell Biol. 2016;214:417–31.
Wrobel L, Sokol AM, Chojnacka M, Chacinska A. The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly. Sci Rep. 2016;6:27484.
Meinecke M, Wagner R, Kovermann P, Guiard B, Mick DU, Hutu DP, et al. Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science. 2006;312:1523–6.
Lytovchenko O, Melin J, Schulz C, Kilisch M, Hutu DP, Rehling P. Signal recognition initiates reorganization of the presequence translocase during protein import. EMBO J. 2013;32:886–98.
Mokranjac D, Neupert W. The many faces of the mitochondrial TIM23 complex. Biochim Biophys Acta. 2010;1797:1045–54.
Wang P, Dalbey RE. Inserting membrane proteins: the YidC/Oxa1/Alb3 machinery in bacteria, mitochondria, and chloroplasts. Biochim Biophys Acta. 2011;1808:866–75.
Hartl FU, Schmidt B, Wachter E, Weiss H, Neupert W. Transport into mitochondria and intramitochondrial sorting of the Fe/S protein of ubiquinol-cytochrome c reductase. Cell. 1986;47:939–51.
Stiller SB, Hopker J, Oeljeklaus S, Schutze C, Schrempp SG, Vent-Schmidt J, et al. Mitochondrial OXA translocase plays a major role in biogenesis of inner-membrane proteins. Cell Metab. 2016;23:901–8.
Park K, Botelho SC, Hong J, Osterberg M, Kim H. Dissecting stop transfer versus conservative sorting pathways for mitochondrial inner membrane proteins in vivo. J Biol Chem. 2013;288:1521–32.
Horst M, Oppliger W, Rospert S, Schonfeld HJ, Schatz G, Azem A. Sequential action of two hsp70 complexes during protein import into mitochondria. EMBO J. 1997;16:1842–9.
Rowley N, Prip-Buus C, Westermann B, Brown C, Schwarz E, Barrell B, et al. Mdj1p, a novel chaperone of the DnaJ family, is involved in mitochondrial biogenesis and protein folding. Cell. 1994;77:249–59.
Ciesielski GL, Plotka M, Manicki M, Schilke BA, Dutkiewicz R, Sahi C, et al. Nucleoid localization of Hsp40 Mdj1 is important for its function in maintenance of mitochondrial DNA. Biochim Biophys Acta. 2013;1833:2233–43.
Frazier AE, Dudek J, Guiard B, Voos W, Li Y, Lind M, et al. Pam16 has an essential role in the mitochondrial protein import motor. Nat Struct Mol Biol. 2004;11:226–33.
Kozany C, Mokranjac D, Sichting M, Neupert W, Hell K. The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase. Nat Struct Mol Biol. 2004;11:234–41.
Sinha D, Joshi N, Chittoor B, Samji P, D'Silva P. Role of Magmas in protein transport and human mitochondria biogenesis. Hum Mol Genet. 2010;19:1248–62.
Bomer U, Meijer M, Maarse AC, Honlinger A, Dekker PJ, Pfanner N, et al. Multiple interactions of components mediating preprotein translocation across the inner mitochondrial membrane. EMBO J. 1997;16:2205–16.
Moro F, Sirrenberg C, Schneider HC, Neupert W, Brunner M. The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix. EMBO J. 1999;18:3667–75.
van der Laan M, Chacinska A, Lind M, Perschil I, Sickmann A, Meyer HE, et al. Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol Cell Biol. 2005;25:7449–58.
Schiller D. Pam17 and Tim44 act sequentially in protein import into the mitochondrial matrix. Int J Biochem Cell Biol. 2009;41:2343–9.
Schendzielorz AB, Schulz C, Lytovchenko O, Clancy A, Guiard B, Ieva R, et al. Two distinct membrane potential-dependent steps drive mitochondrial matrix protein translocation. J Cell Biol. 2017;216:83–92.
Chacinska A, Lind M, Frazier AE, Dudek J, Meisinger C, Geissler A, et al. Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell. 2005;120:817–29.
D'Silva PR, Schilke B, Walter W, Craig EA. Role of Pam16's degenerate J domain in protein import across the mitochondrial inner membrane. Proc Natl Acad Sci U S A. 2005;102:12419–24.
Mokranjac D, Bourenkov G, Hell K, Neupert W, Groll M. Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor. EMBO J. 2006;25:4675–85.
Mokranjac D, Berg A, Adam A, Neupert W, Hell K. Association of the Tim14.Tim16 subcomplex with the TIM23 translocase is crucial for function of the mitochondrial protein import motor. J Biol Chem. 2007;282:18037–45.
D'Silva PR, Schilke B, Hayashi M, Craig EA. Interaction of the J-protein heterodimer Pam18/Pam16 of the mitochondrial import motor with the translocon of the inner membrane. Mol Biol Cell. 2008;19:424–32.
Schilke BA, Hayashi M, Craig EA. Genetic analysis of complex interactions among components of the mitochondrial import motor and translocon in Saccharomyces cerevisiae. Genetics. 2012;190:1341–53.
Ting SY, Yan NL, Schilke BA, Craig EA. Dual interaction of scaffold protein Tim44 of mitochondrial import motor with channel-forming translocase subunit Tim23. Elife. 2017;6, e23609.
Josyula R, Jin Z, Fu Z, Sha B. Crystal structure of yeast mitochondrial peripheral membrane protein Tim44p C-terminal domain. J Mol Biol. 2006;359:798–804.
Marom M, Safonov R, Amram S, Avneon Y, Nachliel E, Gutman M, et al. Interaction of the Tim44 C-terminal domain with negatively charged phospholipids. Biochemistry. 2009;48:11185–95.
Schiller D, Cheng YC, Liu Q, Walter W, Craig EA. Residues of Tim44 involved in both association with the translocon of the inner mitochondrial membrane and regulation of mitochondrial Hsp70 tethering. Mol Cell Biol. 2008;28:4424–33.
Krimmer T, Rassow J, Kunau WH, Voos W, Pfanner N. Mitochondrial protein import motor: the ATPase domain of matrix Hsp70 is crucial for binding to Tim44, while the peptide binding domain and the carboxy-terminal segment play a stimulatory role. Mol Cell Biol. 2000;20:5879–87.
Moro F, Okamoto K, Donzeau M, Neupert W, Brunner M. Mitochondrial protein import: molecular basis of the ATP-dependent interaction of MtHsp70 with Tim44. J Biol Chem. 2002;277:6874–80.
Strub A, Rottgers K, Voos W. The Hsp70 peptide-binding domain determines the interaction of the ATPase domain with Tim44 in mitochondria. EMBO J. 2002;21:2626–35.
D'Silva P, Liu Q, Walter W, Craig EA. Regulated interactions of mtHsp70 with Tim44 at the translocon in the mitochondrial inner membrane. Nat Struct Mol Biol. 2004;11:1084–91.
Demishtein-Zohary K, Gunsel U, Marom M, Banerjee R, Neupert W, Azem A, et al. Role of Tim17 in coupling the import motor to the translocation channel of the mitochondrial presequence translocase. Elife. 2017;6, e22696.
Ting SY, Schilke BA, Hayashi M, Craig EA. Architecture of the TIM23 inner mitochondrial translocon and interactions with the matrix import motor. J Biol Chem. 2014;289:28689–96.
Banerjee R, Gladkova C, Mapa K, Witte G, Mokranjac D. Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein. Elife. 2015;4, e11897.
Schneider HC, Berthold J, Bauer MF, Dietmeier K, Guiard B, Brunner M, et al. Mitochondrial Hsp70/MIM44 complex facilitates protein import. Nature. 1994;371:768–74.
Neupert W, Hartl FU, Craig EA, Pfanner N. How do polypeptides cross the mitochondrial membranes? Cell. 1990;63:447–50.
Glick BS. Can Hsp70 proteins act as force-generating motors? Cell. 1995;80:11–4.
Glick BS, Wachter C, Reid GA, Schatz G. Import of cytochrome b2 to the mitochondrial intermembrane space: the tightly folded heme-binding domain makes import dependent upon matrix ATP. Protein Sci. 1993;2:1901–17.
Voos W, Gambill BD, Guiard B, Pfanner N, Craig EA. Presequence and mature part of preproteins strongly influence the dependence of mitochondrial protein import on heat shock protein 70 in the matrix. J Cell Biol. 1993;123:119–26.
Matouschek A, Azem A, Ratliff K, Glick BS, Schmid K, Schatz G. Active unfolding of precursor proteins during mitochondrial protein import. EMBO J. 1997;16:6727–36.
Matlack KE, Misselwitz B, Plath K, Rapoport TA. BiP acts as a molecular ratchet during posttranslational transport of prepro-alpha factor across the ER membrane. Cell. 1999;97:553–64.
De Los Rios P, Ben-Zvi A, Slutsky O, Azem A, Goloubinoff P. Hsp70 chaperones accelerate protein translocation and the unfolding of stable protein aggregates by entropic pulling. Proc Natl Acad Sci U S A. 2006;103:6166–71.
Sousa R, Liao HS, Cuellar J, Jin S, Valpuesta JM, Jin AJ, et al. Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation. Nat Struct Mol Biol. 2016;23:821–9.
Liu Q, D'Silva P, Walter W, Marszalek J, Craig EA. Regulated cycling of mitochondrial Hsp70 at the protein import channel. Science. 2003;300:139–41.
Marom M, Dayan D, Demishtein-Zohary K, Mokranjac D, Neupert W, Azem A. Direct interaction of mitochondrial targeting presequences with purified components of the TIM23 protein complex. J Biol Chem. 2011;286:43809–15.
Wright PE, Dyson HJ. Intrinsically disordered proteins in cellular signalling and regulation. Nat Rev Mol Cell Biol. 2015;16:18–29.
Schulz C, Rehling P. Remodelling of the active presequence translocase drives motor-dependent mitochondrial protein translocation. Nat Commun. 2014;5:4349.
Li Y, Dudek J, Guiard B, Pfanner N, Rehling P, Voos W. The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18. J Biol Chem. 2004;279:38047–54.
Pais JE, Schilke B, Craig EA. Reevaluation of the role of the Pam18:Pam16 interaction in translocation of proteins by the mitochondrial Hsp70-based import motor. Mol Biol Cell. 2011;22:4740–9.
Aviram N, Ast T, Costa EA, Arakel EC, Chuartzman SG, Jan CH, et al. The SND proteins constitute an alternative targeting route to the endoplasmic reticulum. Nature. 2016;540:134–8.
Avellaneda MJ, Koers EJ, Naqvi MM, Tans SJ. The chaperone toolbox at the single-molecule level: from clamping to confining. Protein Sci. 2017;26:1291–302.
Bausewein T, Mills DJ, Langer JD, Nitschke B, Nussberger S, Kuhlbrandt W. Cryo-EM structure of the TOM core complex from Neurospora crassa. Cell. 2017;170:693–700. e7.
Helpful comments by Szymon Ciesielski are appreciated. This work was supported by National Institutes of Health Grant GM27870.
The author declares she has no competing interests.