Fig. 5

rk-expressing neurons required for the control of pigmentation are located in the VNS. a Abdominal pigmentation in 3-h- (upper panel) and 48-h-old (lower panel) female flies expressing tBur under the control of pan-neuronal driver, elav-GAL4, whose expression was restricted to the brain using tsh-GAL80. Genotypes are coded as described in Fig. 1b. Boxes indicate the first and third quartiles, thick central lines mark the medians, and whiskers represent data range. Red dashed line indicates the median pigmentation level when tBur is expressed ubiquitously (rk>tBur). Results were compared using a one-way ANOVA for each age group and found to not be statistically different (NS). n = 10 for each group. b Representatives pictures of 48-h-old females. Left: elav>tBur fly displaying wing expansion failure and a darker abdomen. Right: elav>tBur; tsh-GAL80 fly displaying normal pigmentation and wings. Scale bar = 1 mm